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Total 2 Articles of 'Keyword: (Garbui) '
Original Research
Obtaining Artificially Caries-affected Dentin for in vitro Studies
Cynthia Soares de Azevedo, Bruna Uglik Garbui, Caroline Martins e Silva, Maria Regina Lorenzetti Simionato, Anderson Zanardi de Freitas, Adriana Bona Matos
[Year:2014] [Month:January-February] [Volumn:15 ] [Number:1] [Pages:136] [Pages No:12-19]
[The Journal of Contemporary Dental Practice]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10024-1480 | FREE


Aim: This study evaluated and improved a protocol for obtaining standard caries-affected dentin (CAD) by Streptococcus mutans biofilm demineralization process.

Materials and methods: Forty-eight human molars were divided in six experimental groups, according to: period of cariogenic challenge (7, 14 or 21 days) and type of dentin (erupted or unerupted teeth). After complete cariogenic challenge sound and CAD dentin were evaluated by: visual inspection (VI), digital radiography (DR), optical coherence tomography (OCT) and laser fluorescence (LF).

Results: Visual inspection confirmed the formation of CAD based on tissue yellowing and loss of surface gloss. Digital radiography detected the presence of radiolucent images, suggesting caries. Three calibrated examiners viewed all images obtained by VI and DR and were able to distinguish healthy from CAD. Fisher’s exact statistical test (p <. 0.05) confirmed no difference between groups by VI (G1/G4: p = 0.6; G2/G5: p = 1; G3/G6: p = 1) or DR (G1/G4: p = 1; G2/G5: p = 1; G3/G6: p = 1). Both LF values and demineralization depth, as determined by OCT, were subjected to ANOVA (p < 0.05). For LF, a statistically significant difference was observed for the type of substrate (p = 0.001). For OCT, no statistically significant differences in the type of substrate (p = 0.163), length of cariogenic challenge (p = 0.512) or interaction between factors (p = 0.148) were observed. Scanning electron micrographs confirmed the presence of CAD; a more uniform demineralization surface was observed in the dentin of unerupted teeth.

Conclusion: This protocol suggests that standard CAD can be obtained in 7 days of cariogenic challenge using unerupted teeth.

Clinical significance: With the new perspective on the clinical treatment of caries lesions, bonding is increasingly performed to demineralize CAD, which is susceptible to remineralization. A useful protocol to standardize the production of CAD, by microbiological cariogenic challenge, would be an important contribution to laboratorial test in the field of operative dentistry.

Keywords: Dental caries, Diagnosis, Caries-affected dentin, Laboratory research.

How to cite this article: Azevedo CS, Garbui BU, Silva CM, Simionato MRL, Freitas AZ, Matos AB. Obtaining Artificially Caries-affected Dentin for in vitro Studies. J Contemp Dent Pract 2014;15(1):12-19.

Source of support: Grants Fapesp/2010/10126-3 and 2010/ 12910-3.

Conflict of interest: None

Original Research - Biomaterials
Comparison of Chemical Aging and Water Immersion Time on Durability of Resin-Dentin Interface Produced by an Etch-and-Rinse Adhesive
Bruna Uglik Garbui, Sergio Brossi Botta, Andre Figueiredo Reis, Adriana Bona Matos
[Year:2012] [Month:July-August] [Volumn:13 ] [Number:4] [Pages:152] [Pages No:464-471]
[The Journal of Contemporary Dental Practice]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10024-1170 | FREE

Aim: The aim of this study was to analyze and compare the influence of short-term NaOCl-storage and long-term water storage on the microtensile bond strength (µTBS) of etch-andrinse adhesive system to human dentin.

Materials and methods: Thirty-six third human molars were randomly divided into 6 groups (n = 6) according to the aging protocol: G1 (water, 24 hours); G2 (water, 6 months); G3 (water, 12 months); G4 (10% sodium hypochlorite – NaOCl, 1 hour); G5 (10% NaOCl, 3 hours) and G6 (10% NaOCl, 5 hours). A two-step etch-and-rinse adhesive (Adper Single Bond 2) was applied according to the manufacturers’ instructions. A composite (Filtek Z250) was applied in four horizontal increments and was individually cured. Specimens were cut following the microtensile test technique, submitted to the different aging protocols, and tested in tension. The fracture pattern was observed in a stereomicroscope (40× magnification) and in a scanning electron microscope. The µTBS data were analyzed by ANOVA and Tukey’s test (? = 0.05).

Results: The effect of storage in 10% NaOCl for 1 or 3 hours was not significantly different from that of aging in distilled water (DW) for 6 or 12 months (p > 0.05). Beams immersed in DW for 24 hours and in 10% NaOCl for 5 hours showed the highest and lowest µTBS values respectively.

Conclusion: The aging protocols negatively influenced dentin bond strength. Aging specimens in 10% NaOCl for 1 or 3 hours can be an alternative method for long-term water storage (6 or 12 months) bond strength studies.

Clinical significance: This aging protocol allows a quick achievement of longitudinal bond strength data, so that results are available to the professionals in this area while the materials are yet present at the dental market.

Keywords: Laboratory research, Dental adhesive, Adhesion, Collagen, Dentin, Degradation, Durability, Microtensile bond test, Sodium hypochlorite.

How to cite this article: Garbui BU, Botta SB, Reis AF, Matos AB. Comparison of Chemical Aging and Water Immersion Time on Durability of Resin-Dentin Interface produced by an Etchand- Rinse Adhesive. J Contemp Dent Pract 2012;13(4):464-471.

Source of support: Nil

Conflict of interest: None declared

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