The scanning electron microscope was used to evaluate the dentin surface of diseased teeth subjected to two methods of conditioning with tetracycline hydrochloride (TTC) for 1 and 4 minutes respectively.  Five groups of twelve specimens each received root planing (control); were immersed in TTC for 1 and 4 minutes; and burnished in TTC for 1 and 4 minutes.  Control specimens exhibited an amorphous irregular surface smear layer.  TTC, irrespective of the method used, was effective in removing the smear layer.  Immersion in TTC for 1 minute revealed obstructed dentinal tubules, while burnishing for 1 minute revealed the presence of collagen fibrils.  The 4-minute application of TTC, irrespective of the conditioning method used, showed wide exposed dentinal tubules.  No significant difference between Groups III, IV, and V was noted (p>0.05).

Keywords:  Tetracycline hydrochloride, TTC, smear layer, collagen fibrils, dentinal tubules

Citation:  Babay N.  SEM Study on The Effect of Two Different Demineralization Methods with Saturated Tetracycline Hydrochloride on Diseased Root Surface.  J Contemp Dent Pract 2001 May;(2)2: 025-035.