Materials and Methods

Dentin Block Preparation

Twenty human teeth extracted due to severe periodontitis were used in the present study.

Following extraction, the border between healthy and diseased root surfaces was marked with a small dental bur.  Following this, each diseased root surface was thoroughly planed with periodontal curettes.  Root dentin exposure was achieved by hand and rotary instrumentation as described by Trombelli et al.¹⁵ Each root was then sectioned longitudinally as three equal parts.  Using a #2 round bur, a horizontal shallow groove was made in a apical-coronal direction on the pulpal surface of every root surface for identification purpose.  The dentin specimens were about 3 x 5 x 2 mm in size.  This yielded a total of sixty dentin specimens; forty-eight experimental and twelve controls.  All specimens were randomly divided into five treatment groups.  The control specimens were obtained from the non-demineralized treated part of the root.  The treatment groups were as follows:

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Citation Number: Vol. 2, No. 2, Page 027