The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 10 , ISSUE 2 ( March, 2009 ) > List of Articles

RESEARCH ARTICLE

The in vitro Antibacterial Effect of Iodine-potassium Iodide and Calcium Hydroxide in Infected Dentinal Tubules in Different Time Intervals

Anda Kfir, Shaul Lin, Amir Laviv, Galit Sela, Zvi Fuss

Citation Information : Kfir A, Lin S, Laviv A, Sela G, Fuss Z. The in vitro Antibacterial Effect of Iodine-potassium Iodide and Calcium Hydroxide in Infected Dentinal Tubules in Different Time Intervals. J Contemp Dent Pract 2009; 10 (2):59-66.

DOI: 10.5005/jcdp-10-2-59

License: CC BY-NC 3.0

Published Online: 01-03-2009

Copyright Statement:  Copyright © 2009; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim

The aim of this study was to evaluate the antibacterial effect of iodine-potassium iodide (IKI) and calcium hydroxide (CH) on dentinal tubules infected with Enterococcus faecalis (E. faecalis) at different time intervals.

Methods and Materials

Hollow cylinders of bovine root dentin (n=45) were infected and divided into three equal groups filled with either IKI or CH and a positive control. After placing each medicament in the infected cylinders for time periods of 10 minutes, 48 hours and 7 days, microbiological samples were analyzed. At the end of each period, four 100 μm thick inner dentin layers (400 μm thick from each specimen) were removed using dental burs of increasing diameters. Dentin powder was cultured on agar plates to quantitatively assess their infection, expressed in colony forming units (cfu).

Results

In all layers of the positive control group, heavy bacterial infection was observed. After 10 minutes, IKI reduced the amount of viable bacteria more efficiently than CH, whereas at later time intervals CH showed the best results.

Conclusion

For short periods of exposure, IKI has a more efficient antibacterial effect in the dentinal tubules than CH but CH performs better after longer durations of exposure.

Clinical Significance

This research indicates the use of IKI is a better choice for disinfecting the root canal than CH if only a short duration of exposure is used because of its more efficient antibacterial effect. However, if a longer exposure time is used, then CH is a better choice because of its better disinfecting effect over time.

Citation

Lin S, Kfir A, Laviv A, Sela G, Fuss Z. The in vitro Antibacterial Effect of Iodine-potassium Iodide and Calcium Hydroxide in Infected Dentinal Tubules in Different Time Intervals. J Contemp Dent Pract 2009 March; (10)2:059-066.


PDF Share
  1. The effect of surgical exposures of dental pulps in germfree and conventional laboratory rats. Oral Surg 1965; 20:340-9.
  2. Ecology of the root canal flora. J Endod 1992; 18:427-30.
  3. The correlation between positive cultures and the prognosis of root canal therapy after pulpectomy. Odontol Revy 1965; 16:193-203.
  4. Bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy. Scand J Dent Res 1981; 89:321-8.
  5. Bacterial invasion of pulpal dentin wall in vitro. J Dent Res 1982; 61:435-8.
  6. The antibacterial action of sodium hypochlorite and EDTA in 60 cases of endodontic therapy. Int Endod J 1985; 18:35-40.
  7. The antibacterial effect of camphorated paramonochlorophenol, camphorated phenol and calcium hydroxide in the treatment of infected root canals. Endod Dent Traumatol 1985; 1:170-5.
  8. Instruments, materials and devices. In: Cohen S, Burns RC (eds). Pathways of the Pulp, 8th edn. St. Louis: Mosby, 2002: 521-73.
  9. Dentinal tubules disinfection using three calcium hydroxide formulations. J Endod 2005; 1:50-2.
  10. Microbiological analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg, Oral Med, Oral Pathol, Oral Radiol, Endod 1998; 85:86-93.
  11. Microbiological status of root-filled teeth with apical periodontitis. Int Endod J 1999; 31:1-7.
  12. Bacteria isolated after unsuccessful endodontic treatment in a North American population. Oral Surg, Oral Med, Oral Pathol, Oral Radiol, Endod 2001; 91:579-86.
  13. Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide. Int Endod J 2002; 35:221-8.
  14. , Rye A, Waltimo T, Ørstavik D, Haapasalo M. Inactivation of root canal medicaments by dentine, hydroxyapatite and bovine serum albumin. Int Endod J 2001; 34:184-8.
  15. In vitro infection and disinfections of dentinal tubules. J Dent Res 1987; 66:1375-9.
  16. Tubular permeability to calcium hydroxide and to bleaching agents. J Endod 1989; 15:362-4.
  17. A laboratory study of the effect of calcium hydroxide mixed with iodine or electrophoretically activated copper on bacterial viability in dentinal tubules. Int Endod J 2002; 35:522-6.
  18. Endodontic medicaments. In: Smith DC, Williams DF (eds). Biocompatibility of Dental Materials. Boca Raton, FL: CRC Press, 1982:223-57.
  19. The antimicrobial effect of calcium hydroxide in root canals pretreated with 5% iodine potassium iodide. Endod Dent Traumatol 1999; 15:205-9.
  20. Root canal dentinal tubule disinfection. J Endod 1990; 16:207-10.
  21. Bacterial penetration and proliferation in root canal dentinal tubules after applying dentin adhesives in vitro. J Endod 2001; 27:398-400.
  22. Assessment of antibacterial activity of endodontic sealers by a direct contact test. Endod Dent Traumatol 1996; 12:179-84.
  23. Disinfection by endodontic irrigants and dressing of experimentally infected dentinal tubules. Endod Dent Traumatol 1990; 6:142-9.
  24. Efficacy of ledermix paste in eliminating Staphylococcus aureus from infected dentinal tubules in vitro. Endod Dent Traumatol 1991; 7:251-4.
  25. Intracanal pH changes of calcium hydroxide pastes exposed to carbon dioxide in vitro. J Endod 1996; 22:362-4.
  26. Inactivation of the antibacterial activity of iodine potassium iodide and chlorhexidine digluconate against E. faecalis by dentin, dentin matrix, type 1 collagen, and heat killed microbial whole cells. J Endod 2002; 28:634-7.
  27. On duration of the antibacterial efficiency of four antiseptics used in root canal treatment in situ. Sven Tandlak Tidskr 1958; 51:1-6.
  28. Biologic effects of endodontic antimicrobial agents. J Endod 1979; 5:166-75.
  29. Chemical and antimicrobial properties of calcium hydroxide mixed with irrigating solutions. Int Endod J 2003; 36:100-5.
  30. Antibacterial efficacy of a new chlorhexidine slow release device to disinfect dentinal tubules. J Endod 2003; 29:416-8.
  31. Endodontic treatment of traumatized teeth. In: Andreasen JO (ed). Traumatic Injuries of the Teeth, 2nd edn. Copenhagen: Munksgaard, 1981: 321-84.
  32. Root resorption – etiology, terminology and clinical manifestations. Endod Dent Traumatol 1988; 4:241-52.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.