The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 13 , ISSUE 3 ( May-June, 2012 ) > List of Articles

RESEARCH ARTICLE

Blood Cells Attachment after Root Conditioning and PRP Application: An in vitro Study

Fabio Renato Manzolli Leite, José Eduardo Cezar Sampaio, Lucas Amaral Fontanari, Eduardo de Paula Ishi, Daniela Leal Zandim, Andrea Abi Rached Dantas, Ricardo Samih Georges Abi Rached

Citation Information : Leite FR, Sampaio JE, Fontanari LA, de Paula Ishi E, Zandim DL, Dantas AA, Rached RS. Blood Cells Attachment after Root Conditioning and PRP Application: An in vitro Study. J Contemp Dent Pract 2012; 13 (3):332-338.

DOI: 10.5005/jp-journals-10024-1147

License: CC BY-NC 3.0

Published Online: 01-06-2012

Copyright Statement:  Copyright © 2012; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim

Root conditioning is aimed at smear layer removal and at dental matrix collagen exposure, which may promote periodontal regeneration. This in vitro study assessed smear layer removal, collagen fiber exposure and the influence of PRP (platelet-rich plasma) application on adhesion of blood cells to the root surface using scanning electron microscopy (SEM).

Materials and methods

Scaled root samples (n = 160) were set in five groups and conditioned with: group I – control group (saline solution); group II (EDTA 24%); group III (citric acid 25%); group IV (tetracycline hydrochloride 50 mg/ml); group V (sodium citrate 30%). Eighty samples were assessed using the root surface modification index (RSMI). The other eighty samples were set in two groups. The first group (n = 40) received PRP gel application with a soft brush and the second group (n = 40) received PRP application and then a blood drop. The fibrin clot formation was assessed in the first group and the blood cells adhesion was assessed in the second group using the BEAI (blood elements adhesion index). A previously trained, calibrated, and blind examiner evaluated photomicrographs. Statistical analysis was performed using the Kruskal-Wallis's and Dunn's tests.

Results

Group III attained the best results for RSMI and BEAI. Moreover, it was the only group showing fibrin clot formation.

Conclusion

Citric acid was the most efficient conditioner for smear layer removal, collagen fiber exposure and blood cell adhesion. Moreover, it was the only group showing fibrin clot formation after PRP application.

Clinical significance

This study demonstrated that root conditioning followed by PRP application may favor blood cell adhesion on root surface which may optimize periodontal healing.

How to cite this article

Dantas AAR, Fontanari LA, Ishi EP, Leite FRM, Zandim DL, Abi Rached RSG, Sampaio JEC. Blood Cells Attachment after Root Conditioning and PRP Application: An in vitro Study. J Contemp Dent Pract 2012;13(3):332-338.


PDF Share
  1. The production of a root surface smear layer by instrumentation and its removal by citric acid. J Periodontol 1984 Aug;55(8):443-46.
  2. The effect of EDTA on the attachment and growth of cultured human gingival fibroblasts in periodontitis-affected root surface. J Contemp Dent Pract 2001 Feb 15;2(1):13-23.
  3. Clinical evaluation of tetracycline HCl conditioning in the treatment of gingival recessions. A comparative study. J Periodontol 1997 Mar;68(3):262-69.
  4. Repair of periodontal furcation defects in beagle dogs following reconstructive surgery including root surface demineralization with tetracycline hydrochloride and topical fibronectin application. J Clin Periodontol 1988 Jan;15(1):73-80.
  5. Scanning electron microscopy of the root surface texture of extracted periodontally diseased teeth following various etching and chelating regimens. Int J Periodontics Restorative Dent 1998 Apr;18(2):171-79.
  6. Blood cell attachment to root surfaces treated with EDTA gel. Braz Oral Res 2005 Apr-Jun;19(2):88-92.
  7. Comparative SEM study on the effect of acid etching with tetracycline HCl or citric acid on instrumented periodontally-involved human root surfaces. J Periodontol 1993 Aug;64(8):689-93.
  8. Effect of tetracycline HCl on periodontally-affected human root surfaces. J Periodontol 1995 Aug;66(8):685-91.
  9. Smear layer removal and collagen fiber exposure using tetracycline hydrochloride conditioning. J Contemp Dent Pract 2008;9(5):25-33.
  10. An in vitro screening model to evaluate root conditioning protocols for periodontal regenerative procedures. J Periodontol 2000 Jul;71(7):1139-43.
  11. Factors related to periodontal regeneration. Periodontol 2000 1993 Feb;1(1):9-15.
  12. Periodontal repair in dogs. Healing patterns in large circumferential periodontal defects. J Clin Periodontol 1991 Jan;18(1):49-59.
  13. Mechanisms initiating platelet thrombus formation. Thromb Haemost 1997 Jul;78(1):611-16.
  14. Platelet gel: An autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillofac Surg 1997 Nov;55(11):1294-99.
  15. The future role of growth factors in bone grafting. Dent Implantol Update 1999 Jan;10(1):5-7.
  16. Tobacco smoking and subgingival dental calculus. J Clin Periodontol 2005 Jan;32(1):81-88.
  17. J Periodontol 2001 May;72(5):666-71.
  18. Effect of different concentrations of EDTA on smear removal and collagen exposure in periodontitis-affected root surfaces. J Clin Periodontol 1997 Aug;24(8):534-37.
  19. Citric acid demineralization of cementum and dentin: The effect of application pressure. J Clin Periodontol 1995 Jun;22(6):434-41.
  20. A comparative scanning electron microscopic study on the characteristics of demineralized dentin root surface using different tetracycline HCl concentrations and application times. J Periodontol 2000 Feb;71(2):219-25.
  21. Effect of Er:YAG and Diode lasers on the adhesion of blood components and on the morphology of irradiated root surfaces. J Periodontal Res 2006 Oct;41(5):381-90.
  22. The effects of different tetracyclines on the dentin root surface of instrumented, periodontally involved human teeth: A comparative scanning electron microscope study. J Periodontol 1997 Aug;68(8):739-45.
  23. The attachment of V79 and human periodontal ligament fibroblasts on periodontally involved root surfaces following treatment with EDTA, citric acid, or tetracycline HCL: An SEM in vitro study. J Contemp Dent Pract 2006 Feb 15;7(1):44-59.
  24. A clinical study of root surface conditioning with an EDTA gel. I. Nonsurgical periodontal treatment. Int J Periodontics Restorative Dent 2000 Dec;20(6):560-65.
  25. SEM study on the effect of two different demineralization methods with saturated tetracycline hydrochloride on diseased root surfaces. J Contemp Dent Pract. 2001 May 15;2(2):25-35.
  26. Platelet volume parameters as a diagnostic tool: The influence of anticoagulation and storage conditions on platelet impedance volume. Klin Wochenschr. 1989 Oct 2;67(19):980-84.
  27. Effects of various root surface treatments on initial clot formation: A scanning electron microscope study. J Nihon Univ Sch Dent 1990 Dec;32(4):281-93.
  28. Collagen linkage in periodontal connective tissue reattachment. An ultrastructural study in beagle dogs. J Periodontol 1988 Nov;59(11):758-68.
  29. Healing of periodontal connective tissues following surgical wounding and application of citric acid in dogs. J Periodontal Res 1980 May;15(3):314-27.
  30. Tetracycline and its derivatives strongly bind to and are released from the tooth surface in active form. J Periodontol 1983 Oct;54(10):580-85.
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.