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VOLUME 13 , ISSUE 4 ( July-August, 2012 ) > List of Articles


Comparison of Chemical Aging and Water Immersion Time on Durability of Resin-Dentin Interface produced by an Etch-and-Rinse Adhesive

Sergio Brossi Botta, Adriana Bona Matos, Bruna Uglik Garbui, Andre Figueiredo Reis

Citation Information : Botta SB, Matos AB, Garbui BU, Reis AF. Comparison of Chemical Aging and Water Immersion Time on Durability of Resin-Dentin Interface produced by an Etch-and-Rinse Adhesive. J Contemp Dent Pract 2012; 13 (4):464-471.

DOI: 10.5005/jp-journals-10024-1170

Published Online: 01-12-2012

Copyright Statement:  Copyright © 2012; The Author(s).



The aim of this study was to analyze and compare the influence of short-term NaOCl-storage and long-term water storage on the microtensile bond strength (μTBS) of etch-andrinse adhesive system to human dentin.

Materials and methods

Thirty-six third human molars were randomly divided into 6 groups (n = 6) according to the aging protocol: G1 (water, 24 hours); G2 (water, 6 months); G3 (water, 12 months); G4 (10% sodium hypochlorite – NaOCl, 1 hour); G5 (10% NaOCl, 3 hours) and G6 (10% NaOCl, 5 hours). A two-step etch-and-rinse adhesive (Adper Single Bond 2) was applied according to the manufacturers¡¦ instructions. A composite (Filtek Z250) was applied in four horizontal increments and was individually cured. Specimens were cut following the microtensile test technique, submitted to the different aging protocols, and tested in tension. The fracture pattern was observed in a stereomicroscope (40× magnification) and in a scanning electron microscope. The μTBS data were analyzed by ANOVA and Tukey's test (α = 0.05).


The effect of storage in 10% NaOCl for 1 or 3 hours was not significantly different from that of aging in distilled water (DW) for 6 or 12 months (p > 0.05). Beams immersed in DW for 24 hours and in 10% NaOCl for 5 hours showed the highest and lowest ìTBS values respectively.


The aging protocols negatively influenced dentin bond strength. Aging specimens in 10% NaOCl for 1 or 3 hours can be an alternative method for long-term water storage (6 or 12 months) bond strength studies.

Clinical significance

This aging protocol allows a quick achievement of longitudinal bond strength data, so that results are available to the professionals in this area while the materials are yet present at the dental market.

How to cite this article

Garbui BU, Botta SB, Reis AF, Matos AB. Comparison of Chemical Aging and Water Immersion Time on Durability of Resin-Dentin Interface produced by an Etchand- Rinse Adhesive. J Contemp Dent Pract 2012;13(4):464-471.

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