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VOLUME 13 , ISSUE 5 ( September-October, 2012 ) > List of Articles


Efficacy of Microwaves and Chlorhexidine for Disinfection of Pacifiers and Toothbrushes: An in vitro Study

Chetan Bhat, Jay Chamele, Tanvi Saraf, Abhijeet Jadhav, Aumir Beg, Ritu Ubeja, Padmeshwari Patil

Citation Information : Bhat C, Chamele J, Saraf T, Jadhav A, Beg A, Ubeja R, Patil P. Efficacy of Microwaves and Chlorhexidine for Disinfection of Pacifiers and Toothbrushes: An in vitro Study. J Contemp Dent Pract 2012; 13 (5):690-694.

DOI: 10.5005/jp-journals-10024-1210

Published Online: 01-02-2013

Copyright Statement:  Copyright © 2012; The Author(s).



The purpose of this study was to evaluate, in vitro, the contamination of toothbrushes and pacifiers by Streptococcus mutans, and the efficacy of microwave and chlorhexidine for their disinfection.

Materials and methods

Sixty pacifiers and 60 toothbrushes were contaminated with S. mutans and then divided into groups according to the disinfection protocol: Group 1—chlorhexidine solution; group 2—microwave sterilization; and group 3—sterile tap water. The devices were evaluated microbiologically after disinfection for the survival of S. mutans colonies and were examined. The results were analyzed statistically by ANOVA and Turkey test.


The results of both types of evaluation showed a large number of S. mutans colonies after spraying with sterile tap water, and chlorhexidine spraying and microwaving were effective in eliminating colonies. Groups 1 and 2 were statistically similar to each other (p > 0.05) and differed significantly from group 3 (p < 0.05).


The 0.12% chlorhexidine solution spray and 7 minutes of microwave irradiation were almost equally effective for disinfection of pacifiers and toothbrushes.

How to cite this article

Chamele J, Bhat C, Saraf T, Jadhav A, Beg A, Jagtap C, Ubeja R, Patil P. Efficacy of Microwaves and Chlorhexidine for Disinfection of Pacifiers and Toothbrushes: An in vitro Study. J Contemp Dent Pract 2012;13(5):690-694.

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  1. Presencia de microrganismos em cepillos dentales utilizados em higiene oral habitual. Rev Dent Chile 1995;85:25-28.
  2. Viability of Streptococcus mutans toothbrush bristles. J Dent Child 2008;75:29-32.
  3. The effects of toothpastes on the residual microbial contamination of toothbrushes. J Am Dent Assoc 2001;132:1241-45.
  4. Effect of triclosan dentifrice on toothbrush contamination. Pediatr Dent 2004;26:11-16.
  5. Bacterial contamination and decontamination of toothbrushes after use. N Y State Dent J 2007;73:20-22.
  6. What is the status of hepatitis C virus infection in France? Gastroenterol Clin Biol 1999;23:349-51.
  7. Hepatitis C contamination of toothbrushes: Myth or reality. J Viral Hepat 2006;13:371-73.
  8. In vitro evaluation of the retention of three species of pathogenic microorganisms by three different types of toothbrush. Oral Microbiol Immunol 2000;15:313-16.
  9. Inhibition of biofilms associated with dentures and toothbrushes by tetrasodium EDTA. J Appl Microbiol 2007;103:2516-24.
  10. Contaminated toothbrushes and their disinfection. J Can Dent Assoc 1995;61:511-16.
  11. Can toothpaste or a toothbrush with antibacterial tufts prevent toothbrush contamination? J Periodontol 2003;74:312-22.
  12. Evaluation of the contamination and disinfection methods of toothbrushes used by 24-to-48-month-old children. J Dent Child 2006;73:152-58.
  13. The efficacy of two UV toothbrush sanitization devices: A pilot study. N Y State Dent J 2008;74:50-52.
  14. Association of maternal-child characteristics as a factor in early childhood caries and salivary bacterial counts. J Dent Child 2006;73:105-11.
  15. Pacifiers: A microbial reservoir. Nurs Health Sci 2006;8:216-23.
  16. Is pacifier use a risk factor for acute otitis media? A dynamic cohort study. Fam Pract 2008;25:233-36.
  17. Allergic rhinitis, feeding and oral habits, tooth-brushing and socioeconomic status: Effects on development of dental caries in primary dentition. Caries Res 2008;42:l4l-47.
  18. Antibacterial effect of electrolyzed water on oral bacteria. J Microbiol 2006;44:417-22.
  19. The efficacy of chlorhexidine spray vs mouthwash in the microbial contamination of child toothbrushes. J Dent Child 2007;74:177-81.
  20. Effect of different exposure times on microwave irradiation on the disinfection of a hard chairside reline resin. J Prosthodont 2008;17:312-17.
  21. Influence of microwave polymerization method and thickness on porosity of acrylic resin. J Prosthodont 2008;17:125-29.
  22. Effect of microwave disinfection on denture base adaptation and resin surface roughness. Braz Dent J 2006;17:195-200.
  23. In vivo evaluation of the bacterial contamination and disinfection of acrylic base plates of removable orthodontic appliances. Am J Orthod Dentofacial Orthop 2007;131:e11-17.
  24. Tipo y grado de contaminación por bacterias bucales y levaduras decepillos dentales con uso habitual. Rev Fac Odontol Univ Chile 1999;17:9-14.
  25. Microbial contamination of toothbrushes and their decontamination. Pediatr Dent 2000;22:381-84.
  26. Chlorhexidine. J West Soc Periodontol Periodontal Abstr 2002;50:5-9.
  27. Bacterial colonization of pacifiers of infants with acute otitis media. J Laryngol Otol 1997;111:614-15.
  28. Relation of oral yeast infection in Brazilian infants and use of a pacifier. J Dent Child 2001;68:33-36.
  29. A study on protozoan cysts, helminth eggs and larvae in pacifiers. J Pediatr 1997;73:21-25.
  30. Prolonged pacifiersucking and use of a nursing bottle at night: Possible risk factors for dental caries in children. Acta Odontol Scand 1998;56:233-37.
  31. Disinfection of pacifiers and toothbrushes. J Pediatric Dent 2011;33:10-13.
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