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VOLUME 13 , ISSUE 6 ( November-December, 2012 ) > List of Articles

RESEARCH ARTICLE

The Efficacy of Neem Extract on Four Microorganisms Responsible for causing Dental Caries viz Streptococcus mutans, Streptococcus salivarius, Streptococcus mitis and Streptococcus sanguis: An in vitro Study

Venkateswara Rao Chava, SM Manjunath, AV Rajanikanth, N Sridevi

Citation Information : Chava VR, Manjunath S, Rajanikanth A, Sridevi N. The Efficacy of Neem Extract on Four Microorganisms Responsible for causing Dental Caries viz Streptococcus mutans, Streptococcus salivarius, Streptococcus mitis and Streptococcus sanguis: An in vitro Study. J Contemp Dent Pract 2012; 13 (6):769-772.

DOI: 10.5005/jp-journals-10024-1227

Published Online: 01-12-2012

Copyright Statement:  Copyright © 2012; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

History and objectives

From the ancient time, neem used to be the traditional medicine for many diseases and was mainly used for cleaning the oral cavity. The incidence of dental caries was less a few decades ago but now the incidence of caries is very aggressive. This might be due to change in dietary habits, life style and more tendency toward processed food. The objective of this study is to find out the truth that if the neem is really efficacious against caries-inducing microorganisms, mainly Streptococcus mutans, Streptococcus salivarius, Streptococcus mitis and Streptococcus sanguis.

Materials and methods

The dried neem sticks ground into a coarse powder and weighed into 5, 10 and 50 gm were added to 100 ml of deionized double distilled water. After soaking for 2 days, the water was filtered at 4°C and the fine filtrate was inoculated onto blood agar plates contains individual species of microorganisms and incubated at 37°C for 2 days.

Results

At maximum concentrations, neem extract has shown the maximum zone of inhibition on Streptococcus mutans. At less concentration, the efficacy of neem has shown some inhibition of growth for all the four species of microorganisms.

Conclusion

Neem chewing provides the maximum benefits. Hence, the use of chewing sticks of neem can be recommended.

How to cite this article

Chava VR, Manjunath SM, Rajanikanth AV, Sridevi N. The Efficacy of Neem Extract on Four Microorganisms Responsible for causing Dental Caries viz Streptococcus mutans, Streptococcus salivarius, Streptococcus mitis, and Streptococcus sanguis: An in vitro Study. J Contemp Dent Pract 2012;13(6):769-772.


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  1. The effect of the extract of the miswak chewing sticks used in Jordan and middle east on oral bacteria. Int Dent J 1995;45(2):18-22.
  2. Essential procedure for clinical microbiology. ASM Press: Washington 1998.
  3. Antibactrial activity of extracts from some African chewing sticks. J Oral Surg 1977;44:717-22.
  4. Preliminary studies on the antibacterial properties of chewing sticks. J Dent 1977;5:123-27.
  5. Primer of biostatistics (4th ed). The McGraw-Hill companies Inc, California 1997.
  6. Evaluation of antiplaque activity of Azadirachta indica leaf extract gel—a 6-week clinical study. J Ethnno Pharmacol 2004;90:99-103.
  7. Indian J Dent Res 1999 Jan-Mar;10(1):23-26.
  8. Chemopreventive potential of neem (Azadirachta indica) on 7,12-dimethylbenz[a] anthracene (DMBA) induced hamster buccal pouch carcinogenesis. J Ethno Pharmacol 1999;67:189-95.
  9. Significance of chewing sticks (Miswaks) in oral hygiene from a pharmacological view-point. JPMA 1981;89-95.
  10. Effect of aqueous extract from neem (Azadirachta indica) on hydrophobicity, biofilm formation and adhesion in composite resin by Candida albicans. Archives Oral Biol 2006;51:482-90.
  11. The effect of mango and neem extract on four organisms causing dental caries: Streptococcus mutans, Streptococcus salivius, Streptococcus mitis, Streptococcus sanguis: An in vitro study. Indian J Dent Res 2007 Oct-Dec;18(4):148-51.
  12. Antimicrobial effects of extracts of Azadirachta indica (Neem) and salvadora persica (arak) chewing sticks Indian J Dent Res 1999;10:23-26.
  13. Antibacterial activity of karanj (Pongamia pinnata) and neem (Azadirachta indica) seed oil: A preliminary report. Microbias 2001;105(412):183-89.
  14. Mania S, Nachani S, Ling S. The inhibiting effect of aqueous Azadirachta indica (neem) extract upon bacterial properties influencing in vitro plaque formation. J Dent Res 1996;75:816-22.
  15. Socioeconomic factors in dental caries prevalence and frequency in Nigerians: An epidemiological study. Caries research 1974;8:155-71.
  16. Native cures in Nigeria, II. The antimicrobial properties of buffered extracts of chewing sticks. Lloydia 1971;34:172-74.
  17. Antimicrobial activity of extracts of some chewing sticks. Oral Surg Oral Med Oral Pathol 1977;44:717-28.
  18. The inhibiting effect of Azadirachta indica against dental pathogens. Asian J Palnt Sci Res 2012:2(1):6-10.
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