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VOLUME 14 , ISSUE 1 ( January-February, 2013 ) > List of Articles


Effect of Three Different Contamination Removal Methods on Bond Strength of a Self-etching Adhesive to Dentin Contaminated with an Aluminum Chloride Hemostatic Agent

Siavash Savadi Oskoee, Mahmoud Bahari, Amir Ahmad Ajami, Mehdi Abed Kahnamoii, Soodabeh Kimyai, Fatemeh Pournaghi-Azar

Citation Information : Oskoee SS, Bahari M, Ajami AA, Kahnamoii MA, Kimyai S, Pournaghi-Azar F. Effect of Three Different Contamination Removal Methods on Bond Strength of a Self-etching Adhesive to Dentin Contaminated with an Aluminum Chloride Hemostatic Agent. J Contemp Dent Pract 2013; 14 (1):26-33.

DOI: 10.5005/jp-journals-10024-1264

Published Online: 01-06-2013

Copyright Statement:  Copyright © 2013; The Author(s).



This study evaluated the effect of three different contamination removal methods on bond strength of one-step self-etching adhesive to dentin contaminated with an aluminum chloride hemostatic agent.

Materials and methods

One hundred noncarious adult molars were used in this study; 2-mm-thick dentin disks were prepared and randomly assigned to 5 groups (n = 20). Group I: control; group II: hemostatic agent-contaminated; group III: hemostatic agent-contaminated and rinsing with water; group IV: hemostatic agent-contaminated and ethylene diamine tetraacetic acid (EDTA) application; and group V: hemostatic agent-contaminated and phosphoric acid application. Clearfil S3 Bond was used to bond composite to dentin surfaces. Subsequent to adding composite cylinders the shear bond strength test was performed. Data were analyzed by one-way ANOVA and Tukey test. Two additional specimens from each group were prepared and evaluated under scanning electron microscope (SEM).


There were statistically significant differences in bond strength among the groups (p < 0.001). In two-by-two comparisons statistically significant differences were observed in bond strength values between all the groups (p < 0.001) except for groups I and IV (p = 0.933).


Aluminum chloride hemostatic agent adversely affected the bond strength of self-etch adhesive to dentin. However, application of EDTA increased the bond strength to the level of normal dentin.

Clinical significance

EDTA and phosphoric acid removed aluminum chloride hemostatic agent contamination of dentin surfaces. However, unlike EDTA phosphoric acid failed to increase the bond strength of self-etch adhesive to dentin to the level of the control group.

How to cite this article

Ajami AA, Kahnamoii MA, Kimyai S, Oskoee SS, Pournaghi-Azar F, Bahari M, Firouzmandi M. Effect of Three Different Contamination Removal Methods on Bond Strength of a Self-etching Adhesive to Dentin Contaminated with an Aluminum Chloride Hemostatic Agent. J Contemp Dent Pract 2013;14(1):26-33.

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