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VOLUME 14 , ISSUE 3 ( May-June, 2013 ) > List of Articles

RESEARCH ARTICLE

Antimicrobial Efficacy of Octenidine Hydrochloride, MTAD and Chlorhexidine Gluconate Mixed with Calcium Hydroxide

Resmiye Ebru Tirali, Kamran Gulsahi, Sevi Burcak Cehreli, Zeynep Ceren Karahan, Emel Uzunoǧlu, Atilla Elhan

Citation Information : Tirali RE, Gulsahi K, Cehreli SB, Karahan ZC, Uzunoǧlu E, Elhan A. Antimicrobial Efficacy of Octenidine Hydrochloride, MTAD and Chlorhexidine Gluconate Mixed with Calcium Hydroxide. J Contemp Dent Pract 2013; 14 (3):456-460.

DOI: 10.5005/jp-journals-10024-1344

Published Online: 01-06-2013

Copyright Statement:  Copyright © 2013; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Objective

The aim of this in vitro study was to investigate whether mixing with calcium hydroxide [Ca(OH)2] affects the antimicrobial action of Octenidine hydrochloride (Octenisept), MTAD and chlorhexidine against Enterococcus faecalis and Candida albicans.

Materials and methods

Freshly grown cultures of Enterococcus faecalis, Candida albicans and a mixture of both strains were incubated in agar plates containing brain-heart infusion broth (BHIB). Zones of inhibition were measured at 24 and 48 hours. Statistical analysis was performed using Mann-Whitney U test and Kruskal-Wallis one-way analysis of variance (ANOVA, both p = 0.05).

Results

Mixing with Ca(OH)2 significantly increased the antibacterial effect of Octenisept (p < 0.05), but did not alter its antifungal activity. Only chlorhexidine showed more antibacterial and antifungal efficiency compared to its Ca(OH)2-mixed version (both p < 0.05). Mixing with Ca(OH)2 decreased the antibacterial efficacy of MTAD, but increased its antifungal effect (both p < 0.05).

Conclusion

These results demonstrate the differential effects of Ca(OH)2 addition on the antimicrobial action of the tested endodontic medicaments in vitro. Ca(OH)2 was as effective as its combination with all of the tested medicaments.

How to cite this article

Tirali RE, Gulsahi K, Cehreli SB, Karahan ZC, Uzunoğlu E, Elhan A. Antimicrobial Efficacy of Octenidine Hydrochloride, MTAD and Chlorhexidine Gluconate Mixed with Calcium Hydroxide. J Contemp Dent Pract 2013;14(3):456-460.


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