Citation Information :
Tirali RE, Gulsahi K, Cehreli SB, Karahan ZC, Uzunoǧlu E, Elhan A. Antimicrobial Efficacy of Octenidine Hydrochloride, MTAD and Chlorhexidine Gluconate Mixed with Calcium Hydroxide. J Contemp Dent Pract 2013; 14 (3):456-460.
The aim of this in vitro study was to investigate whether mixing with calcium hydroxide [Ca(OH)2] affects the antimicrobial action of Octenidine hydrochloride (Octenisept), MTAD and chlorhexidine against Enterococcus faecalis and Candida albicans.
Materials and methods
Freshly grown cultures of Enterococcus faecalis, Candida albicans and a mixture of both strains were incubated in agar plates containing brain-heart infusion broth (BHIB). Zones of inhibition were measured at 24 and 48 hours. Statistical analysis was performed using Mann-Whitney U test and Kruskal-Wallis one-way analysis of variance (ANOVA, both p = 0.05).
Mixing with Ca(OH)2 significantly increased the antibacterial effect of Octenisept (p < 0.05), but did not alter its antifungal activity. Only chlorhexidine showed more antibacterial and antifungal efficiency compared to its Ca(OH)2-mixed version (both p < 0.05). Mixing with Ca(OH)2 decreased the antibacterial efficacy of MTAD, but increased its antifungal effect (both p < 0.05).
These results demonstrate the differential effects of Ca(OH)2 addition on the antimicrobial action of the tested endodontic medicaments in vitro. Ca(OH)2 was as effective as its combination with all of the tested medicaments.
How to cite this article
Tirali RE, Gulsahi K, Cehreli SB, Karahan ZC, Uzunoğlu E, Elhan A. Antimicrobial Efficacy of Octenidine Hydrochloride, MTAD and Chlorhexidine Gluconate Mixed with Calcium Hydroxide. J Contemp Dent Pract 2013;14(3):456-460.
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