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VOLUME 14 , ISSUE 4 ( July-August, 2013 ) > List of Articles

RESEARCH ARTICLE

Comparative Analysis of Colony Counts of Different Species of Oral Streptococci in Saliva of Dentulous, Edentulous and in those Wearing Partial and Complete Dentures

Praveen Kumar Madineni, Suresh Babu Ghanta, Naveen Kumar Motupalli, Mahanthesh Bembalgi, P Krishnam Raju

Citation Information : Madineni PK, Ghanta SB, Motupalli NK, Bembalgi M, Raju PK. Comparative Analysis of Colony Counts of Different Species of Oral Streptococci in Saliva of Dentulous, Edentulous and in those Wearing Partial and Complete Dentures. J Contemp Dent Pract 2013; 14 (4):601-604.

DOI: 10.5005/jp-journals-10024-1371

Published Online: 01-08-2013

Copyright Statement:  Copyright © 2013; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Objectives

To study and compare the number of colony forming units of Streptococcus mutans, Streptococcus sanguis, Streptococcus salivarius, Streptococcus mitis and Streptococcus milleri in dentulous, edentulous and in those wearing partial and complete dentures by using semi-quantitative culture method of saliva samples with calibrated standard loop

Materials

Sterile specimen collection bottles, Mitis salivarius agar plates, Standard loop, Candle jar, Incubator, Colony counter

Methodology

Study population consisted of 100 subjects with 25 in each group, with an age range of 40 to 80 years, who were attending the Department of Community Dentistry and Prosthodontics at MNR Dental College, Sangareddy, Hyderabad. Unstimulated saliva samples were collected from patients and inoculated on to Mitis salivarius agar plates using calibrated standard loop. The plates were then incubated anaerobically at 37°C for 24 hours and left at room temperature for further 24 hours. Using a colony counter, the number of colonies of each species was counted.

Results

Streptococcus mutans and Streptococcus mitis predominates in the dentulous group, Streptococcus sanguis in complete denture group, Streptococcus salivarius in edentulous group and Streptococcus milleri in removable partial denture group.

Conclusion

The results of our study are in accordance with the previous studies, which have sought to differentiate different groups of mutans streptococci using a simple calibrated standard loop.

How to cite this article

Ealla KKR, Ghanta SB, Motupalli NK, Bembalgi M, Madineni PK, Raju PK. Comparative Analysis of Colony Counts of Different Species of Oral Streptococci in Saliva of Dentulous, Edentulous and in those Wearing Partial and Complete Dentures. J Contemp Dent Pract 2013;14(4):601-604.


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