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VOLUME 14 , ISSUE 5 ( September-October, 2013 ) > List of Articles

RESEARCH ARTICLE

An in vitro comparison of Antimicrobial Efficacy of Three Root Canal Irrigants—BioPure MTAD, 2% Chlorhexidine Gluconate and 5.25% Sodium Hypochlorite as a Final Rinse against E. faecalis

MS Rama Rao, Vinod Agrawal, Kanupriya Dhingra, V Rajesh Gopal, Abhijita Mohapatra, Abhilash Mohapatra

Citation Information : Rao MR, Agrawal V, Dhingra K, Gopal VR, Mohapatra A, Mohapatra A. An in vitro comparison of Antimicrobial Efficacy of Three Root Canal Irrigants—BioPure MTAD, 2% Chlorhexidine Gluconate and 5.25% Sodium Hypochlorite as a Final Rinse against E. faecalis. J Contemp Dent Pract 2013; 14 (5):842-847.

DOI: 10.5005/jp-journals-10024-1413

Published Online: 00-10-2013

Copyright Statement:  Copyright © 2013; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim

This study was conducted to evaluate the antimicrobial activity of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) and BioPure MTAD when used as a final rinse against Enterococcus faecalis.

Materials and methods

Sixty single-rooted premolars were biomechanically prepared, inoculated with E. faecalis and divided into various groups. These were then irrigated with the test irrigants and tested microbiologically for growth of E. faecalis immediately after irrigation and after 48 hours.

Results

Statistical analysis showed that there was a significant difference between the antibacterial activities of BioPure MTAD, 2% CHX and 5.25% NaOCl at 5 minutes; however, the antibacterial activities of the three irrigants were comparable after 2 days of irrigation

Conclusion

The present study concludes that BioPure MTAD is as effective against E. faecalis as 5.25% NaOCl and more effective than 2% CHX.

Clinical significance

E. faecalis is one of the most resistant intracanal species and a possible cause of root canal failure. Many authors have stressed the importance of using antimicrobial irrigants during chemomechanical preparation to ensure complete disinfection. Therefore, various irrigating solutions have been used during and immediately after root canal preparation to remove debris and necrotic pulp tissue and to eliminate microorganisms that cannot be reached by mechanical instrumentation.

How to cite this article

Agrawal V, Rao MSR, Dhingra K, Gopal VR, Mohapatra A, Mohapatra A. An in vitro comparison of Antimicrobial Efficacy of Three Root Canal Irrigants—BioPure MTAD, 2% Chlorhexidine Gluconate and 5.25% Sodium Hypochlorite as a Final Rinse against E. faecalis. J Contemp Dent Pract 2013;14(5):842-847.


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