The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 14 , ISSUE 5 ( September-October, 2013 ) > List of Articles

RESEARCH ARTICLE

Evaluating the Antimicrobial Activity of Commercially Available Herbal Toothpastes on Microorganisms Associated with Diabetes Mellitus

Reena Kulshrestha, J Kranthi, P Krishna Rao, Feroz Jenner, V Abdul Jaleel, G Maheswar

Citation Information : Kulshrestha R, Kranthi J, Rao PK, Jenner F, Jaleel VA, Maheswar G. Evaluating the Antimicrobial Activity of Commercially Available Herbal Toothpastes on Microorganisms Associated with Diabetes Mellitus. J Contemp Dent Pract 2013; 14 (5):924-929.

DOI: 10.5005/jp-journals-10024-1427

Published Online: 00-10-2013

Copyright Statement:  Copyright © 2013; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim

The present study was conducted to evaluate the efficacy of commercially available herbal toothpastes against the different periodontopathogens.

Materials and methods

Six herbal toothpastes that were commonly commercially available were included in the study. Colgate herbal, Babool, Meswak, Neem active, Dabur red toothpastes were tested for the study whereas sterile normal saline was used as control. Antimicrobial efficacies of dentifrices were evaluated against Streptococcus mutans and Actinobacillus actinomycetemcomitans. The antimicrobial properties of dentifrices were tested by measuring the maximum zone of inhibition at 24 hours on the Mueller Hinton Agar media inoculated with microbial strain using disk diffusion method. Each dentifrice was tested at 100% concentration (full strength).

Results

The study showed that all dentifrices selected for the study were effective against the entire test organism but to varying degree. Neem active tooth paste gave a reading of 25.4 mm as the zone of inhibition which was highest amongst all of the test dentifrices. Colgate Herbal and Meswak dentifrices recorded a larger maximum zone of inhibition, measuring 23 and 22.6 mm respectively, compared to other toothpastes. All other dentifrices showed the zone of inhibition to be between 17 and 19 mm respectively.

Conclusion

The antibacterial properties of six dentifrices were studied in vitro and concluded that almost all of the dentifrices available commercially had antibacterial properties to some extent to benefit dental health or antiplaque action.

How to cite this article

Jenner F, Jaleel VA, Kulshrestha R, Maheswar G, Rao PK, Kranthi J. Evaluating the Antimicrobial Activity of Commercially Available Herbal Toothpastes on Microorganisms Associated with Diabetes Mellitus. J Contemp Dent Pract 2013;14(5):924-929.


PDF Share
  1. Spices oils for the control of co-occurring mycotoxin producing fungi. J Food Protection 2002;65:638-687.
  2. Alliin lyase localization in bundle sheaths of garlic clove (Allium sativum). American J Botany 1994;81:89-94.
  3. Periodontal microbial ecology. Periodontol 2000 2005;38:135-187.
  4. The microbiology of primary dental caries in humans. J Dent Educ 2001;65:1028-1037.
  5. Effect of chlorhexidine-thymol varnish on root caries in a geriatric population: a randomized double-blind clinical trial. J Dent 2009;37:679-685.
  6. The effect of sodium hypochlorite and chlorhexidine on cultured human periodontal ligament cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001;92:446-450.
  7. Immediate hypersensitivity to chlorhexidine: literature review. Eur Ann Allergy Clin Immunol 2004;36:123-126.
  8. Unconventional dentistry: Part IV. Unconventional dental practices and products. J Can Dent Assoc 2000;66(10):564-568.
  9. The antibacterial effect of toothpastes on the salivary flora. J Clin Periodontol 1988;15(3):193-199.
  10. Understanding “alternative” dental products. Gen Dent 2001;49(6):616-620; quiz 621-622.
  11. Book on constituents of bacteriological culture media. Cambridge University Press; 1956.
  12. Methods for the isolation and identification of obligate anaerobes. European Journal of Clinical Microbiology and Infectious Diseases 1992;11:1039-1043.
  13. Periodontopathogens: bacteriology of periodontal disease: mini review: Journal of Pure and Applied Microbiology, 2013 March;7(1):583-586.
  14. Bergey's manual of determinative bacteriology, 8th ed. The Williams and Wilkins Co. Baltimore, 1974.
  15. Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria: Approved Standard M11-A7. CLSI, Wayne, PA, USA, 2007.
  16. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45:493-496.
  17. Comparison of efficacy of three antibiotic discs. Indian Journal of Medical Microbiology 2008;26(2):160-162.
  18. Evaluating the antiplaque efficacy of herbal dentifrices—an in vitro study. Int JA PS. BMS 2012;1(2):150-159.
  19. Bacteriology of dental caries: formal discussion. J Dent 1964;43:1045-1047.
  20. Transmission of periodontal bacteria and models of infections. J Clin Periodontal 2005;32(suppl)6:16-27.
  21. Selective bacterial adherence to oral epithelial surfaces and its role as an ecological determinant. Infect Immun 1990;3(4):567-573.
  22. Oral microflora. In: Busscher (editor). Oral biofilms and plaque control. 1st ed. AITBS Publication, 1998;222-231.
  23. The antimicrobial potential of 14 natural herbal dentifrices: results of an in vitro diffusion method study. J Am Dent Assoc 2004;135(8):1133-1141.
  24. The antibacterial properties of some commercially available toothpastes in vitro. Journal of Dental Research 1984;156:175.
  25. Efficacy of herbal toothpaste on patients with established gingivitis—a randomized controlled trial. Braz Oral Res 2006;20(2):172-177.
  26. The technology behind Colgate Total toothpaste. Oral Diseases 2005;11(Suppl 1):98-121.
  27. In vitro antibacterial comparison of dentifrices claiming antigingivitis and gumhealing properties. Abstract # 0389. Tom‘s of Maine, Kennebunk, Maine, USA. Available from URL: http://www.mattmetzgar.com/matt_metzgar/files/AntigingivitisAntibacterialComparison.
  28. Paraclinical effects of Miswak extract on dental plaque. J Dent Res 2007;4(2):106-110.
  29. Adv in Med Dent Sci 2008;2(3):61-65.
  30. Toothpaste formulation efficacy in reducing oral flora. Tropical Journal of Pharmaceutical Research, February 2009;8(1):71-77.
  31. Comparative evaluation of the antimicrobial efficacy of aloe vera tooth gel and two popular commercial toothpastes: an in vitro study. Gen Dent 2009;57:238-241.
  32. Antimicrobial efficacy of different toothpastes and mouthrinses: an in vitro study. Dent Res J (Isfahan). 2011 Spring;8(2):85-94.
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.