The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 15 , ISSUE 1 ( January-February, 2014 ) > List of Articles

RESEARCH ARTICLE

Obtaining Artificially Caries-affected Dentin for in vitro Studies

Adriana Bona Matos, Bruna Uglik Garbui, Cynthia Soares de Azevedo, Caroline Martins e Silva, Maria Regina Lorenzetti Simionato, Anderson Zanardi de Freitas

Citation Information : Matos AB, Garbui BU, de Azevedo CS, Silva CM, Simionato MR, de Freitas AZ. Obtaining Artificially Caries-affected Dentin for in vitro Studies. J Contemp Dent Pract 2014; 15 (1):12-19.

DOI: 10.5005/jp-journals-10024-1480

Published Online: 01-08-2014

Copyright Statement:  Copyright © 2014; The Author(s).


Abstract

Aim

This study evaluated and improved a protocol for obtaining standard caries-affected dentin (CAD) by Streptococcus mutans biofilm demineralization process.

Materials and methods

Forty-eight human molars were divided in six experimental groups, according to: period of cariogenic challenge (7, 14 or 21 days) and type of dentin (erupted or unerupted teeth). After complete cariogenic challenge sound and CAD dentin were evaluated by: visual inspection (VI), digital radiography (DR), optical coherence tomography (OCT) and laser fluorescence (LF).

Results

Visual inspection confirmed the formation of CAD based on tissue yellowing and loss of surface gloss. Digital radiography detected the presence of radiolucent images, suggesting caries. Three calibrated examiners viewed all images obtained by VI and DR and were able to distinguish healthy from CAD. Fisher's exact statistical test (p < 0.05) confirmed no difference between groups by VI (G1/G4: p = 0.6; G2/G5: p = 1; G3/G6: p = 1) or DR (G1/G4: p = 1; G2/G5: p = 1; G3/G6: p = 1). Both LF values and demineralization depth, as determined by OCT, were subjected to ANOVA (p < 0.05). For LF, a statistically significant difference was observed for the type of substrate (p = 0.001). For OCT, no statistically significant differences in the type of substrate (p = 0.163), length of cariogenic challenge (p = 0.512) or interaction between factors (p = 0.148) were observed. Scanning electron micrographs confirmed the presence of CAD; a more uniform demineralization surface was observed in the dentin of unerupted teeth.

Conclusion

This protocol suggests that standard CAD can be obtained in 7 days of cariogenic challenge using unerupted teeth.

Clinical significance

With the new perspective on the clinical treatment of caries lesions, bonding is increasingly performed to demineralize CAD, which is susceptible to remineralization. A useful protocol to standardize the production of CAD, by microbiological cariogenic challenge, would be an important contribution to laboratorial test in the field of operative dentistry.

How to cite this article

Azevedo CS, Garbui BU, Silva CM, Simionato MRL, Freitas AZ, Matos AB. Obtaining Artificially Caries-affected Dentin for in vitro Studies. J Contemp Dent Pract 2014;15(1):12-19.


PDF Share
  1. A doença e seu tratamento clínico. São Paulo: Editora Santos, 2011.
  2. Effect of Er, Cr:YSGG laser on the microtensile bond strength of two different adhesives to the sound and caries-affected dentin. Oper Dent 2009 Jul-Aug;34(4):460-466.
  3. Effect of 2% chlorhexidine digluconate on the bond strength to normal versus caries-affected dentin. Oper Dent 2009 Mar-Apr;34(2):157-165.
  4. Effect of chlorhexidine pretreatment on bond strength durability of caries-affected dentin over 2-year aging in artificial saliva and under simulated intrapulpal pressure. Oper Dent 2011 Nov-Dec;36(6):649-660.
  5. Effect of pretest storage conditions of extracted teeth on their dentin bond strengths. J Pros Dent 2010 Aug;104(2):92-97.
  6. Effect of chlorhexidine concentrations on micro-shear bond strength of self-etch adhesive to normal and caries-affected dentin. Am J Dent 2010 Aug;23(4):217-222.
  7. Bond strengths of a one-step self-etching system to caries-affected and normal dentin. Oper Dent 2006 Nov-Dec;31(6):677-681.
  8. Microtensile bond strength of three simplified adhesive systems to caries-affected dentin. J Adhes Dent 2010 Aug;12(4):273-278.
  9. Effect of adhesive systems associated with resin-modified glass ionomer cements. J Oral Rehabil 2006 Feb;33(2):110-116.
  10. Dentin Caries Zones: Mineral, Structure and Properties. J Dent Resear 2009 Jan;88(1):71-76.
  11. Microtensile bond strength of two-step etchand- rinse adhesive systems on sound and artificial caries-affected dentin. Am J Dent 2010 Jun;23(3):152-156.
  12. Mineral density, morphology and bond strength of natural versus artificial caries-affected dentin. Dent Mater J 2013;32(1):138-143.
  13. Artificial methods of dentin caries induction: a hardness and morphological comparative study. Archives of Oral Biology 2009 Dec;54(12):1111-1117.
  14. An in vitro microbial model for studying secondary caries formation. Caries Res 1996;30(2):112-118.
  15. Evaluation of three different adhesive systems using a bacterial method to develop secondary caries in vitro. Am J Dent 2010 Apr;23(2):93-97.
  16. Resistance to degradation of bonded restorations to simulated caries-affected primary dentin. Am J Dent 2010 Feb;23(1):47-52.
  17. Exposed collagen in aged resin-dentin bonds produced on sound and caries-affected dentin in the presence of chlorhexidine. J Adhes Dent 2011 Apr;13(2):117-124.
  18. Evaluation of caries-affected dentin with optical coherence tomography. Braz Oral Res 2011 Sep-Oct;25(5):407-413.
  19. Structure and microstructure of coronary dentin in non-erupted human deciduous incisor teeth. Braz Dent J 2002;13(3):170-174.
  20. Streptococcus mutans induced secondary caries adjacent to glass ionomer cement, composite resin and amalgam restorations in vitro. Braz Oral Res 2007 Oct-Dec;21(4):368-374.
  21. The effect of distance and tooth structure on laser fluorescence caries detection. Oper Dent 2012 Mar-Apr;37(2):150-160.
  22. Laboratorial method proposal to obtain caries-affected dentin. Saarbrucken/Germany: LAP Lambert Academic Publisher; 2013.
  23. Influence of sterilization method on the bond strength of caries-affected dentin. Braz Oral Res 2009 Jan-Mar;23(1):11-16.
  24. Dentin bonding-variables related to the clinical situation and the substrate treatment. Dental Materials 2010 Feb;26(2):E24-E37.
  25. The density and branching of dentinal tubules in human teeth. Arch Oral Biol 1996 May;41(5):401-412.
  26. Performance of a new fluorescence camera for detection of occlusal caries in vitro. Lasers Med Sci 2012 Mar 21.
  27. Performance of laser fluorescence devices, visual and radiographic examination for the detection of occlusal caries in primary molars. Clin Oral Investig 2011 Oct;15(5):635-641.
  28. Performance of fluorescence-based and conventional methods of occlusal caries detection in primary molars—an in vitro study. Int J Paediatr Dent 2012 Nov;22(6):459-466.
  29. A clinical study of a laser fluorescence device for the detection of approximal caries in primary molars. Int J Paediatr Dent 2012 Mar;22(2):132-138.
  30. Performance of a pen-type laser fluorescence device and conventional methods in detecting approximal caries lesions in primary teeth—in vivo study. Caries Research 2009;43(1):36-42.
  31. Diagnosis of dentin involvement in occlusal caries based on visual and radiographic examination of the teeth. Scandinavian J Dent Resear 1992 Jun;100(3):144-148.
  32. A study of a laser fluorescence device for assessing caries removal in primary teeth in vitro. Hua Xi Kou Qiang Yi Xue Za Zhi 2011 Oct;29(5):457-460.
  33. In situ and in vitro comparison of laser fluorescence with visual inspection in detecting occlusal caries lesions. Lasers Med Sci 2011 Jan;26(1):1-5.
  34. In vitro comparison of laser fluorescence performance with visual examination for detection of occlusal caries in permanent and primary molars. Lasers Med Sci 2009 Jul;24(4):501-506.
  35. Performance of fluorescence methods, radiographic examination and ICDAS II on occlusal surfaces in vitro. Caries Res 2008;42(4):297-304.
  36. Nondestructive assessment of inhibition of demineralization in dental enamel irradiated by a lambda = 9.3 microm CO2 laser at ablative irradiation intensities with PS-OCT. Lasers in Surgery and Medicine 2008 Jul;40(5):342-349.
  37. Polarization-sensitive optical coherence tomographic imaging of artificial demineralization on exposed surfaces of tooth roots. Dental Materials 2009 Jun;25(6):721-728.
  38. Optical coherence tomography diagnostic imaging. General Dentistry 2008 Nov;56(7):750-757.
  39. Determination of dental decay rates with optical coherence tomography. Laser Physics Letters 2009 Dec;6(12):896-900.
  40. Communication among oral bacteria. Microbiology and Molecular Biology Reviews 2002 Sep;66(3):486.
  41. Bacterial diversity in human subgingival plaque. J Bacteriol 2001 Jun;183(12):3770-3783.
  42. Glucan-binding proteins of the oral streptococci. Crit Rev Oral Biol Med 2003;14(2):89-99.0007
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.