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VOLUME 15 , ISSUE 3 ( May-June, 2014 ) > List of Articles


Genetic Association for Caries Susceptibility among Cleft Lip and/or Palate Individuals

Leonardo Santos Antunes, Patricia Nivoloni Tannure, Lívia Azeredo Alves Antunes, Maria Fernanda Reis, Marcelo Castro Costa, Cresus Vinicius Depes Gouvêa, Beni Olej, Jose Mauro Granjeiro

Citation Information : Antunes LS, Tannure PN, Antunes LA, Reis MF, Costa MC, Gouvêa CV, Olej B, Granjeiro JM. Genetic Association for Caries Susceptibility among Cleft Lip and/or Palate Individuals. J Contemp Dent Pract 2014; 15 (3):288-293.

DOI: 10.5005/jp-journals-10024-1530

Published Online: 01-12-2014

Copyright Statement:  Copyright © 2014; The Author(s).



To evaluate the association of the polymorphisms in the TGFB3 gene (rs2268626), and the BMP4 gene (rs17563) with dental caries in two different groups (noncleft group and oral cleft group) from a cleft center located at Rio de Janeiro, Brazil.

Materials and methods

A total of 486 unrelated children and adolescents with or without caries were evaluated using a cohort design. Data on oral health habits was obtained through a questionnaire and caries data was collected by clinical examination. Genotyping of the selected polymorphisms for TGFB3 and BMP4 were carried out by real-time PCR using the TaqMan assay method from a genomic DNA isolated from buccal epithelial cells of all children and adolescents.


No association was found between BMP4 polymorphism and caries among individuals from both groups. For TGFB3 polymorphism, significant differences were observed for allele and genotype frequencies between caries free and caries affected individuals in oral cleft group (p = 0.013 and 0.006 for allele and genotype frequencies respectively).


Our findings provide evidence suggesting that TGFB3 may be involved in caries susceptibility in oral cleft group.

Clinical significance

In the future, the possibility of identifying genes related to caries susceptibility can lead to counseling of the individual that carries gene alterations, with the aim of working on preventive measures for caries as well as bioengineering treatments.

How to cite this article

Antunes LS, Tannure PN, Antunes LAA, Reis MF, Costa MC, Gouvêa CVD, Olej B, Granjeiro JM, Küchler EC. Genetic Association for Caries Susceptibility among Cleft Lip and/or Palate Individuals. J Contemp Dent Pract 2014;15(3):288-293.

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