Antimicrobial Activity of Root Canal Irrigants associated with Cetrimide against Biofilm and Planktonic Enterococcus faecalis
Mario Tanomaru-Filho, Camila Almeida Nascimento, Norberto Batista Faria-Junior, Gisele Faria, Juliane Maria Guerreiro-Tanomaru
Citation Information :
Tanomaru-Filho M, Nascimento CA, Faria-Junior NB, Faria G, Guerreiro-Tanomaru JM. Antimicrobial Activity of Root Canal Irrigants associated with Cetrimide against Biofilm and Planktonic Enterococcus faecalis. J Contemp Dent Pract 2014; 15 (5):603-607.
To evaluate the antibacterial activity of sodium hypochlorite (NaOCl) and chlorhexidine (CHX) alone or associated with cetrimide (CTR), and QMiX against biofilm and planktonic Enterococcus faecalis (E. faecalis) [American type culture collection (ATCC) 29212].
Materials and methods
The solutions 2.5% NaOCl, 2.5% NaOCl + 0.2% CTR, 2% CHX, 2% CHX + 0.2% CTR, 0.2% CTR, and QMiX were evaluated. E. faecalis biofilms were induced for 14 days on bovine dentin blocks. The irrigants were evaluated after contact with E. faecalis suspension and biofilm for 1 and 3 minutes. After that, serial decimal dilutions were made and plated on tryptic soy agar (TSA) medium. Plates were incubated for 24 hours at 37°C and the colony-forming unit (CFU) 1 ml was determined. Data were subjected to ANOVA and Tukey's tests at 5% significance.
Results
All microorganisms were eliminated by direct contact of the irrigants with planktonic cells. Only NaOCl and NaOCl + CTR were able to completely eliminate the microorganisms by direct contact with E. faecalis biofilm. CHX presented effectiveness similar to CHX + CTR CTR, and QMiX after 1 minute of contact and similar to NaOCl and NaOCl + CTR after 3 minutes (p > 0.05), but was unable to completely eliminate the microorganisms. CTR and QMiX did not differ from each other.
Conclusion
CTR addition to CHX and NaOCl solutions did not improve the antimicrobial activity against biofilm. All evaluated irrigants and associations presented activity against planktonic E. faecalis. Only NaOCl and NaOCl + CTR eliminated biofilm after 1 and 3 minutes of direct contact.
Clinical relevance
Addition of CTR does not modify the antibiofilm effectiveness of CHX and NaOCl.
How to cite this article
Nascimento CA, Tanomaru-Filho M, Faria-Junior NB, Faria G, Guerreiro-Tanomaru JM. Antimicrobial Activity of Root Canal Irrigants associated with Cetrimide against Biofilm and Planktonic Enterococcus Faecalis. J Contemp Dent Pract 2014;15(5):603-607.
Clinical implications and microbiology of bacterial persistence after treatment procedures. J Endod 2008 Nov;34(11):1291-1301.
Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J 1997 Sep;30(5):297-306.
Antibacterial effectiveness of several irrigating solutions and the Endox Plus system: an ex vivo study. Int Endod J 2012 Dec;45(12):1091-1096.
Antibacterial efficacy of endodontic irrigating solutions and their combinations in root canals contaminated with Enterococcus faecalis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011 Sep;112(3):396-400.
Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 2006 Feb;32(2):93-98.
Antimicrobial effect of endodontic solutions used as final irrigants on a dentin biofilm model. Int Endod J 2012 Feb;45(2):162-168.
Tissue dissolution by sodium hypochlorite: effect of concentration, temperature, agitation and surfactant. J Endod 2010 Sep;36(9):1558-1562.
Effect of mechanical stress on biofilms challenged by different chemicals. Water Res 2005 Dec;39(20):5142-5152.
The effect of detergents on the antibacterial activity of disinfecting solutions in dentin. J Endod 2012 Jul;38(7):948-953.
Residual and antimicrobial activity of final irrigation protocols on Enterococcus faecalis biofilm in dentin. J Endod 2011 Mar;37(3):363-366.
Eradication of Enterococcus faecalis biofilms by cetrimide and chlorhexidine. J Endod 2010 Jan;36(1):87-90.
Antimicrobial activity of maleic acid and combinations of cetrimide with chelating agents against Enterococcus faecalis biofilm. J Endod 2010 Oct;36(10):1673-1675.
A new noninvasive model to study the effectiveness of dentin disinfection by using confocal laser scanning microscopy. J Endod 2011 Oct;37(10):1380-1385.
The antimicrobial effect of new and conventional endodontic irrigants on intraorally infected dentin. Acta Odontol Sc and 2013 May-Jul;71(3-4):424-431.
Antibacterial and smear layer removal ability of a novel irrigant, QMiX. Int Endod J 2012 Apr;45(4):363-371.
In vitro antibacterial action of Tetraclean, MTAD and five experimental irrigation solutions. Int Endod J 2010 Jun;43(6):528-535.
Biofilm dissolution and cleaning ability of different irrigant solutions on intraorally infected dentin. J Endod 2011 Aug;37(8):1134-1138.
Comparative analysis of Enterococcus faecalis biofilm formation on different substrates. J Endod 2013 Mar;39(3):346-350.
Evaluation of pH, available chlorine content, and antibacterial activity of endodontic irrigants and their combinations against Enterococcus faecalis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011 Jul;112(1):132-135.
Antimicrobial effect of conventional root canal medicaments vs propolis against Enterococcus faecalis, Staphylococcus aureus and Candida albicans. J Contemp Dent Pract 2012;13:305-309.
Biofilm formation capability of Enterococcus faecalis cells in starvation phase and its susceptibility to sodium hypochlorite. J Endod 2010 Apr;36(4):630-635.
Observations of biofilm growth on human dentin and potential destruction after exposure to antibiotics. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008 Apr;105(4):526-529.
Antimicrobial efficacy of chlorhexidine against bacteria in biofilms at different stages of development. J Endod 2011 May;37(5):657-661.
Effectiveness of endodontic disinfecting solutions against young and old Enterococcus faecalis biofilms in dentin canals. J Endod 2012 Oct;38(10):1376-1379.
Eradication of enterococci biofilms by lactic acid alone and combined with chlorhexidine and cetrimide. Med Oral Patol Oral Cir Bucal 2012 Sep 1;17(5):e902-e906.
Susceptibilties of two Enterococcus faecalis phenotypes to root canal medications. J Endod 2005 Jan;31(1):30-36.
Comparative evaluation of antimicrobial efficacy of sodium hypochlorite, MTAD, and Tetraclean against Enterococcus faecalis biofilm. J Endod 2007 Jul;33(7):852-855.
In vitro antimicrobial activity of several concentrations of sodium hypochlorite and chlorhexidine gluconate in the elimination of Enterococcus faecalis. Int Endod J 2001 Sep;34(6):424-428.
Comparative study of the antimicrobial effect of three irrigant solutions (chlorhexidine, sodium hypochlorite and chlorhexidinated MUMS). J Contemp Dent Pract 2012 Jul 1;13(4):436-439.
Killing of Enterococcus faecalis by MTAD and chlorhexidine digluconate with or without cetrimide in the presence or absence of dentin powder or BSA. J Endod 2006 Feb;32(2):138-141.
Comparative evaluation of endodontic irrigants against Enterococcus faecalis biofilms. J Endod 2006;32:527-531.
The effect of exposure to irrigant solutions on apical dentin biofilms in vitro. J Endod 2006 May;32(5):434-437.
Inactivation of local root canal medicaments by dentin: an in vitro study. Int Endod J 2000 Mar;33(2):126-131.
Dentin inhibits the antibacterial effect of new and conventional endodontic Irrigants. J Endod 2013 Mar;39(3):406-410.
Inactivation of the antibacterial activity of iodine potassium iodide and chlorhexidine digluconate against Enterococcus faecalis by dentin, dentin matrix, type-I collagen, and heat-killed microbial whole cells. J Endod 2002 Sep;28(9):634-637.
