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VOLUME 15 , ISSUE 6 ( November-December, 2014 ) > List of Articles

RESEARCH ARTICLE

Influence of Metalloproteinases on Dentin Hybridization of One-bottle or Self-etch Dental Bonding Systems

Sérgio Luiz Pinheiro, Diana Roberta Pereira, Flávia De Milito, Karina Teixeira Villalpando

Citation Information : Pinheiro SL, Pereira DR, Milito FD, Villalpando KT. Influence of Metalloproteinases on Dentin Hybridization of One-bottle or Self-etch Dental Bonding Systems. J Contemp Dent Pract 2014; 15 (6):705-711.

DOI: 10.5005/jp-journals-10024-1603

Published Online: 01-12-2014

Copyright Statement:  Copyright © 2014; The Author(s).


Abstract

Aim

To assess the influence of dentin substrate and chlorhexidine on the marginal seal of composite resin restorations.

Materials and methods

The sample comprised 20 third molars. Four cavities were drilled in the dentin surface, followed by sealing and restoration of sound dentin (n = 10) or carious dentin (n = 10). In the control group, cavities were immediately restored as follows: G1: one-bottle bonding agent (OB) + composite resin (CR); G2: chlorhexidine 2.0% (CLX) + OB + CR; G3: self-etch bonding agent (SE) + CR; G4: CLX + SE + CR. In the experimental group (carious dentin), carious lesions were induced with S. mutans and cavities were restored as in the control group. Five specimens from each group (sound and carious) were stored in brain–heart infusion (BHI) medium for 6 months. All specimens were submerged in methylene blue 0.5% to test for microleakage. The Kruskal-Wallis and Student- Newman-Keuls tests were used to assess results.

Results

On immediate assessment, there were no significant between-group differences in the sound dentin group, whereas in carious dentin, there was less leakage when OB and CLX were used. At 6 months, there was less leakage in the sound dentin group when OB and CLX were used; there were no between-group differences in carious dentin. Comparison of immediate and 6-month assessments showed a significant increase in leakage at 6 months when sound dentin was treated with SE and CLX and when carious dentin was treated with OB and CLX.

Conclusion

To ensure better dentin hybridization and preservation of the organic constituents of the dentin matrix, the properties of chlorhexidine digluconate and the components of the resin matrix must be taken into account.

Clinical significance

Metalloproteinases influence degradation of the hybrid layer in composite resin restorations, regardless of whether the bonding system used is one-bottle or self-etching.

How to cite this article

Pinheiro SL, Pereira DR, Milito FD, Villalpando KT. Influence of Metalloproteinases on Dentin Hybridization of One-bottle or Self-etch Dental Bonding Systems. J Contemp Dent Pract 2014;15(6):705-711.


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  1. M atrix m etalloproteinases a nd t issue inhibitors of metalloproteinases: structure, function, and biochemistry. Circ Res 2003;92(8):827-839.
  2. Matrix Metalloproteinases: a review. Crit Rev Oral Biol Med 1993;4(2):197-250.
  3. The role of matrix metalloproteinases in dental erosion. Adv Dent Res 2012;24(2):72-76.
  4. Genetic variation in MMP20 contributes to higher caries experience. J Dent 2012;40(5):381-386.
  5. The matrix metalloproteinase gelatinase a in human dentine. Arch Oral Biol 2000;45(9):757-765.
  6. The localization of matrix metalloproteinase-20 (MMP-20, enamelysin) in mature human teeth. J Dent Res 2002;81(9):603-607.
  7. Matrix metalloproteinase-8 (MMP-8) is the major collagenase in human dentin. Arch Oral Biol 2007;52(2):121-127.
  8. Zymographic analysis and characterization of MMP-2 and -9 forms in human sound dentin. J Dent Res 2007;86(5):436-440.
  9. The incorporation of chlorhexidine in a two-step self-etching adhesive preserves dentin bond in vitro. J Dent 2009;37(10):807-812.
  10. Inhibition of the activities of matrix metalloproteinases 2, 8 and 9 by chlorhexidine. Clin Diagn Lab Immunol 1999;6(3):437-439.
  11. Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo. J Dent Res 2005;84(8):741-746.
  12. In vivo preservation of the hybrid layer by chlorhexidine. J Dent Res 2007;86(6):529-533.
  13. Effect of 2% chlorhexidine digluconate on the bond strength to normal versus cariesaffected dentin. Oper Dent 2009;34(2):157-165.
  14. Effect of chlorhexidine on the bond strength of a self-etch adhesive system to sound and demineralized dentin. Braz Oral Res 2013;27(3):218-224.
  15. Does inhibition of proteolytic activity improve adhesive luting? Eur J Oral Sci 2013;121(2):121-131.
  16. Confocal laser scanning microscopic analysis of the depth of dentin caries-like lesions in primary and permanent teeth. Braz Dent J 2008;19(2):139-144.
  17. In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model. Eur J Clin Nutr 2005;59(7):884-886.
  18. Performance of Brazilian and imported glass ionomer cements used in atraumatic restorative treatment (ART) regarding microleakage in primary molars. J Appl Oral Sci 2006;14(5):312-318.
  19. Flowable glass ionomer cement as a liner: improving marginal adaptation of atraumatic restorative treatment restorations. J Dent Child 2010;77(1):12-16.
  20. The effect of polishing systems on microleakage of tooth coloured restoratives: Part 1. Conventional and resin-modified glass-ionomer cements. J Oral Rehabil 2000;27(2):117-123.
  21. Dentin treatment with MMPs inhibitors does not alter bond strengths to cariesaffected dentin. J Dent 2008;36(12):1068-1073.
  22. Microtensile bond strength of total-etch and self-etching adhesives to caries-affected dentine. J Dent 2003;31(7):469-477.
  23. In vivo degradation of resin-dentin bonds in humans over 1 to 3 years. J Dent Res 2000;79(6):1385-1391.
  24. The activation and function of host matrix metalloproteinases in dentin matrix breakdown in caries lesions. J Dent Res 1998;77(8):1622-1629.
  25. Collagen degradation by host-derived enzymes during aging. J Dent Res 2004;83(3):216-221.
  26. Effect of chlorhexidine application in a self-etching adhesive on the immediate resin-dentin bond strength. J Adhe Dent 2010;12(1):27-31.
  27. Effect of pretreatment with mildly acidic hypochlorous acid on adhesion to caries-affected dentin using a self-etch adhesive. Eur J Oral Sci 2011;119(1):86-92.
  28. Improving the effect of NaOCl pretreatment on bonding to caries-affected dentin using self-etch adhesives. J Dent 2009;37(10):769-775.
  29. Nanomechanical properties of hydrated carious human dentin. J Dent Res 2001;80(8):1768-1771.
  30. The ultrastructure and hardness of the transparent layer of human carious dentin. J Dent Res 1983;62(1):7-10.
  31. Tensile bond strength and SEM evaluation of caries-affected dentin using dentin adhesives. J Dent Res 1995;74(10):1679-1688.
  32. Effects of storage media on mechanical properties of adhesive systems. Am J Dent 2004;17(2):104-108.
  33. Effects of additional and extended acid etching on bonding to caries-affected dentine. Eur J Oral Sci 2004;112(5):458-464.
  34. Microtensile bond strength of two-step etch-and-rinse adhesive systems on sound and artificial caries-affected dentin. Am J Dent 2010;23(3):152-156.
  35. Histomorphologic characterization and bond strength evaluation of caries-affected dentin/resin interfaces: effects of long-term water exposure. Dent Mater 2008;24(6):786-798.
  36. A preliminary study of activation of collagenase in carious human dentine matrix. Arch Oral Biol 1983;28(2):185-187.
  37. Differential expression of matrix metalloproteinase-2 in human coronal and radicular sound and carious dentine. J Dent 2010;38(8):635-640.
  38. Chemical profile of adhesive/caries-affected dentin interfaces using Raman microspectroscopy. J Biomed Mater Res A 2007;81(2):279-286.
  39. Effects of chlorhexidine on dentin surfaces and shear bond strengths. Am J Dent 1994;7(2):81-84.
  40. H+ induced tension development in demineralized dentin matrix. J Dent Res 2000;79(8):1579-1783.
  41. Immunocytochemical identification of type I collagen in acid-etched dentin. J Biomed Mater Res A 2003;66(4):764-769.
  42. The effect of chlorhexidine on dentin hybrid layers in vivo. Oper Dent 2007;32(2):107-111.
  43. Inhibition of enzymatic degradation of adhesive-dentin interfaces. J Dent Res 2009;88(12):1101-1106.
  44. A 2-year in vitro evaluation of a chlorhexidine-containing acid on the durability of resin-dentin interfaces. J Dent 2011;39(1):40-47.
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