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VOLUME 15 , ISSUE 6 ( November-December, 2014 ) > List of Articles


Analysis of the Proliferative Potential of Odontogenic Epithelial Cells of Pericoronal Follicles

Natalia Cimadon, Isabel Silva Lauxen, Vinicius Coelho Carrard, Manoel Sant'Ana Filho, Pantelis Varvaki Rados, Márcia Gaiger Oliveira

Citation Information : Cimadon N, Lauxen IS, Carrard VC, Filho MS, Rados PV, Oliveira MG. Analysis of the Proliferative Potential of Odontogenic Epithelial Cells of Pericoronal Follicles. J Contemp Dent Pract 2014; 15 (6):761-765.

DOI: 10.5005/jp-journals-10024-1613

Published Online: 01-04-2015

Copyright Statement:  Copyright © 2014; The Author(s).



To evaluate the proliferative potential and the cell proliferation rate of odontogenic epithelial cells.

Materials and methods

Forty-two cases of pericoronal follicles of impacted third molars were submitted to silver impregnation technique for quantification of argyrophilic nucleolar organizer regions (AgNOR) and immunohistochemical staining for EGFR and Ki-67. For AgNOR quantification, the mean number of active nucleolar organizer regions per nucleus (mAgNOR) and the percentage of cells with 1, 2, 3 and 4 or more AgNORs per nucleus (pAgNOR) were quantified. Ki-67 immunolabeling was quantified, whereas for EGFR, a descriptive analysis of staining patterns (membrane, cytoplasm or membrane + cytoplasm positivity) was performed. We evaluated the reduced epithelium of the enamel organ and/or islands of odontogenic epithelium present in the entire connective tissue.


mAgNOR were 1.43 (1.0-2.42) and were significantly different among pericoronary follicles from upper and lower teeth (p = 0.041). Immunostaining of Ki-67 was negative in all cases. EGFR immunolabeling was found mainly in the cytoplasm and was more intense in islands and cords when compared to reduced epithelium of the enamel organ.


Odontogenic epithelial cells of some pericoronal follicles have proliferative potential, suggesting their association with the development of odontogenic lesions.

Clinical significance

The authors suggest that nonerupted, especially of the lower teeth, should be monitored and if necessary removed.

How to cite this article

Cimadon N, Lauxen IS, Carrard VC, Filho MSA, Rados PV, Oliveira MG. Analysis of the Proliferative Potential of Odontogenic Epithelial Cells of Pericoronal Follicles. J Contemp Dent Pract 2014;15(6):761-765.

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  1. The WHO histological typing of odontogenic tumors. A commentary on the second edition. Cancer 1992;70(12):2988-2994.
  2. Cysts associated with long-standing third molars. Int J Oral Maxillofac Surg 1993;22(2):110-112.
  3. A long-term follow-up radiographic evaluation of asymptomatic impacted third molars in orthodontically treated patients. Int J Oral Maxillofac Surg 1994;23(5):279-285.
  4. Diseases and lesions associated with third molars: review of 1001 cases. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1995;79(2):142-145.
  5. Pathologically significant pericoronal lesions in adults: histopathologic evaluation. J Oral Maxillofac Surg 2002;60(6):613-617.
  6. Pathological changes and immunoexpression of p63 gene in dental follicles of asymptomatic impacted lower third molars: an immunohistochemical study. J Craniofac Surg 2010;21(3):854-857.
  7. A clinicoradiographic and pathological study of pericoronal follicles associated to mandibular third molars. J Cranifac Surg 2014;25(3):e283-287.
  8. MIB-1 expression in odontogenic epithelial rests, epithelium of health oral mucosa and epithelium of selected odontogenic cysts: na immunohistochemical study. Int J Oral Maxillofac Surg 2005;34(4):432-435.
  9. Pericoronal follicles of asymptomatic impacted teeth: a radiographic, histomorphologic, and immunohistochemical study. Int J Dent 2012;(2012):955310.
  10. Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolarorganiser regions at the optical level. Histochem J 1986;18(1):5-14.
  11. Ki-67 immunohistochemistry: a valuable marker in prognostication but with a risk of misclassification: proliferation subgroups formed based on Ki-67 immunoreactivity and standardized mitotic index. Histopathology 2006;48(6):674-682.
  12. Immunohistochemical localization of the epidermal growth factor receptor in normal human tissues. Lab Invest 1986;55(5):588-592.
  13. EGFR and p53 expression and proliferative activity in parathyroid adenomas; an immunohistochemical study. APMIS 2001;109(12):870-874.
  14. Review of epidermal growth factor receptor biology. Int J Radiol Oncol Biol Phys 2004;59(2 suppl):21-26.
  15. Epidermal growth factor receptor distribution in pericoronal follicles: relationship with the origin of odontogenic cysts and tumors. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007;103(2):240-245.
  16. The hypothesis of ‘focal ameloblastoma’. Med Oral Patol Oral Cir Bucal 2013;18(1):e60-64.
  17. Pathologic change in soft tissues associated with radiographically ‘normal’ third molar impactions. Br J Oral Maxill Surg 1999;37(4):259-260.
  18. Cystic changes in dental follicle associated with radiographically normal impacted mandibular third molar. Br J Oral Maxillofac Surg 2008;46(7):552-553.
  19. Indications for surgical removal of the mandibular third molar. Swed Dent J 1987;11(1-2):23-29.
  20. Age of patients and morbidity associated with mandibular third molar surgery. J Am Dent Assoc 1980;101(2):240-245.
  21. Nucleolar function and size in cancer cells. Am J Pathol 1998;152(5):1291-1297.
  22. Nucleolar size indicates the rapidity of cell proliferation in cancer tissues. J Pathol 2000;191(2):181-186.
  23. Nucleolar organizer regions (AgNORs) in odontogenic cysts and ameloblastomas. J Oral Pathol Med 1996;25(8):436-440.
  24. Nucleolar organizer regions in selected odontogenic lesions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003;95(2):187-192.
  25. Actual proliferating index and p53 protein expression as prognostic marker in odontogenic cysts. Oral Dis 2009;15(7):490-498.
  26. Quantitative and qualitative analysis of argyrophilic nuclear organizer regions in follicular cyst, keratocystic odontogenic tumor and ameloblastoma. J Cancer Res Ther 2011;7(3):280-285.
  27. Odontogenic Cysts: Analysis of 680 Cases in Brazil. Head and Neck Pathol 2008;2(3):150-156.
  28. Benign tumors of the jaws: a 10-year retrospective analysis. Rev Cir Traumatol Buco Maxillofac 2010;10(1):91-96.
  29. Odontogenic tumors: analysis of 127 cases. Pesqui Odontol Bras 2001;15(4):308-313.
  30. Ki-67 and MCM2 in dental follicle and odontogenic cysts: the effects of inflammation on proliferative markers. Scientific World J 2012;(2012):946060.
  31. Odontogenic epithelium: immunolabeling of Ki-67, EGFR and Survivin in Pericoronal Follicles, Dentigerous Cysts and Keratocystic Odontogenic Tumors. Head Neck Pathol 2011;5(1):1-7.
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