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VOLUME 16 , ISSUE 10 ( October, 2015 ) > List of Articles

RESEARCH ARTICLE

Fibrinogen Demonstration in Oral Lichen Planus: An Immunofluorescence Study on Archival Tissues

Alka Kale, Pallavi D Shirol, Veena Naik

Citation Information : Kale A, Shirol PD, Naik V. Fibrinogen Demonstration in Oral Lichen Planus: An Immunofluorescence Study on Archival Tissues. J Contemp Dent Pract 2015; 16 (10):824-828.

DOI: 10.5005/jp-journals-10024-1764

Published Online: 01-10-2015

Copyright Statement:  Copyright © 2015; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Background

Lichen planus is a premalignant condition with minimal diagnostic aids. This study is an attempt to use paraffin embedded sections of lichen planus with immunofluorescein stain and to evaluate the immunofluorescent sections to establish pattern of fibrinogen deposition.

Materials and methods

Thirty-five paraffin embedded sections of old and new cases of oral lichen planus (study group) and five normal oral mucosa (control group) were chosen. Two sections of each (H & E) case were taken, one was stained with hematoxylin and eosin and another with fluorescein isothiocynate conjugate (FITC) polyclonal rabbit antibody against fibrinogen. Fluorescent findings were examined with a fluorescent microscope.

Results

A high statistical significant correlation was found in respect to fluorescence positivity, intensity of fluorescence and distribution of fluorescence each with p < 0.0001 and fluorescence at blood vessel walls (p = 0.0003).

Conclusion

This study suggested that paraffin embedded sections can be successfully used in direct immunofluorescence staining in routine set up where only formalin fixed tissues are received.

Clinical significance

Paraffin embedded sections can be successfully used in direct immunofluorescence staining when only formalin fixed tissues are received.

How to cite this article

Shirol PD, Naik V, Kale A. Fibrinogen Demonstration in Oral Lichen Planus: An Immunofluorescence Study on Archival Tissues. J Contemp Dent Pract 2015;16(10): 824-828.


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