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VOLUME 16 , ISSUE 11 ( November, 2015 ) > List of Articles


Influence of Concentration and Agitation of Sodium Hypochlorite and Peracetic Acid Solutions on Tissue Dissolution

Mario Tanomaru-Filho, Juliane Maria Guerreiro-Tanomaru, Bruna Ramos Franco Silveira, Roberta Bosso Martelo

Citation Information : Tanomaru-Filho M, Guerreiro-Tanomaru JM, Silveira BR, Martelo RB. Influence of Concentration and Agitation of Sodium Hypochlorite and Peracetic Acid Solutions on Tissue Dissolution. J Contemp Dent Pract 2015; 16 (11):876-879.

DOI: 10.5005/jp-journals-10024-1775

Published Online: 01-04-2016

Copyright Statement:  Copyright © 2015; The Author(s).


Aim and objective

To evaluated the tissue dissolution of sodium hypochlorite (NaOCl) and peracetic acid (PA) solutions at different concentrations, with or without ultrasonic agitation.

Materials and methods

The following solutions were analyzed: 2.5% NaOCl, 0.5, 1 and 2% PA, 1% PA associated with 6.5% hydrogen peroxide (HP) and saline. Fragments of bovine pulp tissue with 25 ± 2g mg were immersed into test tubes containing 4 mL of the solutions for 10 minutes. In the groups with agitation, pulp tissues were submitted to 2 cycles of 1 minute of ultrasonic agitation. The specimens were weighed after the removal from the solutions. The percentage of mass loss was calculated according to the difference of mass before and after exposure to solutions. Data were submitted to ANOVA and Tukey tests (p . 0.05).


A total of 2.5% NaOCl with or without agitation showed the higher tissue dissolution (between 64.5 and 67% of mass reduction) (p < 0.005). By comparing the PA solutions, the concentrations of 1 and 2% with or without agitation and the concentration of 0.5% with agitation showed similar dissolution activity (between 35.4 and 44% of mass reduction). The use of the ultrasonic agitation promoted an increase of the dissolution ability only for 0.5% PA.


Peracetic acid solution has pulp tissue dissolution. However, this ability is lower than 2.5% NaOCl solution.

Clinical significance

The sodium hypochlorite solution shows higher ability to dissolve tissue than PA.

How to cite this article

Tanomaru-Filho M, Silveira BRF, Martelo RB, Guerreiro-Tanomaru JM. Influence of Concentration and Agitation of Sodium Hypochlorite and Peracetic Acid Solutions on Tissue Dissolution. J Contemp Dent Pract 2015;16(11):876-879.

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