Effect of Achyranthes aspera, 0.2% Aqueous Chlorhexidine Gluconate and Punica granatum Oral Rinse on the Levels of Salivary Streptococcus mutans in 8 to 12 Years Old Children
Deepak Goel, Aayushi Bansal, Anant Gopal Nigam
Citation Information :
Goel D, Bansal A, Nigam AG. Effect of Achyranthes aspera, 0.2% Aqueous Chlorhexidine Gluconate and Punica granatum Oral Rinse on the Levels of Salivary Streptococcus mutans in 8 to 12 Years Old Children. J Contemp Dent Pract 2015; 16 (11):903-909.
To study the effect Achyranthes aspera, 0.2% aqueous chlorhexidine gluconate and Punica granatum oral rinse on salivary Streptococcus mutans count in children.
Materials and methods
A total of 60 children of 8 to 12 years of age were randomly allocated into 3 groups. Group A was given 0.2% chlorhexidine mouthwash, group B was given 10% A. aspera mouthwash and group C was given 15% P. granatum mouthwash. The day 1 saliva samples were collected from the subjects and innoculated onto mitis salivarius bacitracin (MSB) agar. The colony counts were obtained by a clinical microbiologist who was blinded to the subject allocation. Plaque scores were then recorded by the investigator with the help of a volunteer. Following this, they received a thorough scaling and polishing. Subjects in each group were then provided with 140 ml of the respective mouthwash, as a daily supervised rinse after breakfast and before sleeping as per instructions. Following mouth rinsing, the children were instructed not to eat or drink for 15 minutes. At the 7th day, unstimulated saliva was again collected from the subjects of all 3 groups, inoculated onto MSB agar and colony count was obtained. Modified Quigley- Hein plaque index was also evaluated for the refreshed score at this stage. Colony counting was done using loop method and statistical analysis was done using Statistical Package for the Social Science (SPSS) software version 21.
All the three mouthwashes showed statistically significant reduction of S. mutans count and plaque index after 7 days, i.e. chlorhexidine (p < 0.001 for reduction in S. mutans count and p < 0.05 for plaque score reduction), A. aspera (p < 0.01 for reduction in S. mutans count and p < 0.05 for plaque score reduction) and P. granatum (p < 0.01 for reduction in S. mutans count and p < 0.05 for plaque score reduction). Chlorhexidine had marginally better results in reducing S. mutans count.
• Efficacy of chlorhexidine, A. aspera and P. granatum was statistically significant with respect to reduction of S. mutans count with chlorhexidine being marginally better than the other two,
• All the three mouthwashes were found to be at par when plaque index values from baseline and after interception of 7 days was calculated,
• Punica granatum has better antimicrobial effect than A. aspera.
How to cite this article
Bansal A, Marwah N, Nigam AG, Goenka P, Goel D. Effect of Achyranthes aspera, 0.2% Aqueous Chlorhexidine Gluconate and Punica granatum Oral Rinse on the Levels of Salivary Streptococcus mutans in 8 to 12 Years Old Children. J Contemp Dent Pract 2015;16(11):903-909.
Chlorhexidine: a medicine for all the oral diseases. Global J Med Public Health 2012;1(2):43-48.
Biguanides as industrial biocides. In:Page KR, editor. Industrial biocides. Chichester: John Wiley; 1988. p. 19-36.
Chlorhexidine: is it still the gold standard? Periodontol 2000 1997;15:55-62.
Role of selected Indian plants in management of type 2 diabetes: a review. J Altern Complement Med 2004;10:369-378.
Screening of antimicrobial activity of plants popularly used in Guatemala for treatment of dermatomucosal diseases. J Ethnopharmacol 1987;20:223-237.
Ogden LG, et al. Safety and antioxidant activity of a pomegranate ellagitannin enriched polyphenol dietary supplement in overweight individuals with increased waist size. J Agric Food Chem 2007;55:10050-10054.
Preliminary evaluation of anti-inflammatory and anti-arthritic activity of S lappa, A speciosa, A aspera. Phytomedicine 2002;9(5):433-437.
A review of the literature on use of chlorhexidine in dentistry. J Am Dent Assoc 1986;112:863-869.
Triclosan: a potential allergen in sutureline allergic contact dermatitis. Dermatol Surg 2009;35:888-889.
Triclosan. Dermatitis 2006;17:204-207.
Efficacy of two alcohol-free cetylpyridinium chloride mouthwashes.a randomized double-blind crossover study. J Clin Periodontol 2008;35:230-235.