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VOLUME 16 , ISSUE 11 ( November, 2015 ) > List of Articles

RESEARCH ARTICLE

Prevalence of Herpesvirus and Correlation with Clinical Parameters in Indian Subjects with Chronic Periodontitis

Vinayak Mahableshwar Joshi, Kishore Gajanan Bhat, Sandeep Suresh Katti, Manohar Suresh Kugaji, Preeti Shivaji Ingalgi

Citation Information : Joshi VM, Bhat KG, Katti SS, Kugaji MS, Ingalgi PS. Prevalence of Herpesvirus and Correlation with Clinical Parameters in Indian Subjects with Chronic Periodontitis. J Contemp Dent Pract 2015; 16 (11):915-920.

DOI: 10.5005/jp-journals-10024-1781

Published Online: 00-11-2015

Copyright Statement:  Copyright © 2015; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Objective

The identification of new uncultured species and viruses supports the possibility of combination of the herpesvirus- bacterial periodontal infection for periodontitis. The paucity of data and studies with larger sample size in Indian subjects provides an unclear picture of the presence of the herpesvirus in this population.

Materials and methods

This was a cross-sectional study consisting of 100 each in the healthy group and chronic periodontitis (CP) group. The subgingival plaque was collected and polymerase chain reaction was performed post deoxyribonucleic acid (DNA) extraction by using specific primers for human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV). The data were analyzed using Fisher's exact test, Mann-Whitney U test and Spearman's coefficient correlation.

Results

Human cytomegalovirus and EBV viruses were significantly higher in the CP group as compare to the healthy group. A higher percentage of those with CMV positive had EBV also positive (28.3%) compared to only 9.1% of CMV negative being EBV positive in the CP group. When both the healthy and CP group in total was compared, there was a significant correlation with all clinical parameters.

Conclusion

Both the viruses dominated in disease as compared to health were similar to the earlier findings. The CP group had higher pocket depth and clinical attachment loss in the virus positive subjects. These findings could suggest that virus serves as a prelude to the disease and the combination of the two viruses could play a role in the pathogenesis.

How to cite this article

Joshi VM, Bhat KG, Katti SS, Kugaji MS, Ingalgi PS. Prevalence of Herpesvirus and Correlation with Clinical Parameters in Indian Subjects with Chronic Periodontitis. J Contemp Dent Pract 2015;16(11):915-920.


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  1. Microbial etiological agents of destructive periodontal diseases. Periodontol 2000 1994;5:78-111.
  2. Human viruses in periodontitis. Periodontol 2000 2010;53:89-110.
  3. Biology and pathogenesis of cytomegalovirus in periodontal disease. Periodontol 2000 2014;64:40-56.
  4. Herpesviralbacterial interrelationships in aggressive periodontitis. J Periodontal Res 2004;39:207-212.
  5. Relationship between herpesviruses and adult periodontitis and periodontopathic bacteria. J Periodontol 1999;70:478-484.
  6. Detection of herpetic viruses in gingival crevicular fluid of patients suffering from periodontal diseases: prevalence and effect of treatment. Oral Microbiol Immunol 2009;24:506-509.
  7. Salivary levels of Epstein-Barr virus DNA correlate with subgingival levels, not severity of periodontitis. Oral Dis 2009;15:554-559.
  8. Association between human herpesviruses and the severity of periodontitis. J Periodontol 2004 Nov;75:1479-1485.
  9. Comparative analysis of presence of cytomegalovirus (CMV) and Epstein-Barr virus-1 (EBV-1) in cases of chronic periodontitis and aggressive periodontitis with controls. Ind J Dent Res 2012;23:454-458.
  10. Herpesviruses in chronic and aggressive periodontitis patients in an Indian population. J Oral Sci 2009;51:79-86.
  11. Prevalence of some herpesviruses in gingival crevicular fluid. J Clin Virol 2005;34:147-152.
  12. Realtime polymerase chain reaction quantification of human cytomegalovirus and Epstein-Barr virus in periodontal pockets and the adjacent gingiva of periodontitis lesions. J Periodontal Res 2005;40:97-104.
  13. Development of a classification system for periodontal diseases and conditions. Ann Periodontol 1999;4:1-6.
  14. Principles and technical aspects of PCR amplification. Netherlands: Springer; 2008. p. 34.
  15. Detection and typing of HSV-1, HSV-2, CMV and EBV by quadruplex PCR. Yonsei Med J 2003;44:1001-1007.
  16. Chromatin control of herpes simplex virus lytic and latent infection. Nat Rev Microbiol 2008;6:211-221.
  17. Role of herpes simplex-1, Epstein-Barr and human cytomegaloviruses in aggressive periodontitis. Int Jol Dent Res 2013;1:19-24.
  18. EBV and CMV in chronic periodontitis: a prevalence study. Arch Viro 2008;153:1917-1919.
  19. Human cytomegalovirus and Epstein-Barr virus in apical and marginal periodontitis: a role in pathology? J Med Virol 2008;80:1007-1011.
  20. Detection of herpesviruses and periodontal pathogens in subgingival plaque of patients with chronic periodontitis, generalized aggressive periodontitis, or gingivitis. J Periodontol 2008;79:2313-2321.
  21. Human herpesvirus 7, Epstein-Barr virus and human cytomegalovirus in periodontal tissues of periodontally diseased and healthy subjects. J Clin Periodontol 2008;35:831-837.
  22. Herpesviruses in periodontal pocket and gingival tissue specimens. Oral Microbiol Immunol 2000;15:15-58.
  23. Subgingival human cytomegalovirus correlates with increased clinical periodontal parameters and bacterial coinfection in periodontitis. J Periodontol 2007;78:2303-2310.
  24. Real-time polymerase chain reaction quantification of Epstein-Barr virus in chronic periodontitis patients. J Periodontal Res 2005;40:294-298.
  25. Realtime polymerase chain reaction quantification of human cytomegalovirus and Epstein-Barr virus in periodontal pockets and the adjacent gingiva of periodontitis lesions. J Periodontal Res 2005;40:97-104.
  26. Herpesvirus in localized juvenile periodontitis. J Periodontal Res 2000;35:17-25.
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