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VOLUME 16 , ISSUE 2 ( February, 2015 ) > List of Articles

RESEARCH ARTICLE

Titanium Surface Roughing Treatments contribute to Higher Interaction with Salivary Proteins MG2 and Lactoferrin

Yuri Wanderley Cavalcanti, Rodrigo Villamarim Soares, Marina Araújo Leite Assis, Elton Gonçalves Zenóbio, Francisco Mauro da Silva Girundi

Citation Information : Cavalcanti YW, Soares RV, Assis MA, Zenóbio EG, da Silva Girundi FM. Titanium Surface Roughing Treatments contribute to Higher Interaction with Salivary Proteins MG2 and Lactoferrin. J Contemp Dent Pract 2015; 16 (2):141-146.

DOI: 10.5005/jp-journals-10024-1651

Published Online: 01-02-2015

Copyright Statement:  Copyright © 2015; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Purpose

This study aimed to evaluate the interactions between salivary proteins and titanium disks with different surface treatments.

Materials and methods

Machined titanium disks (n = 48) were divided into four experimental groups (n = 12), according to their surface treatments: surface polishing (SP); acid etching (A); spot-blasting plus acid etching (SB-A); spot-blasting followed by acid etching and nano-functionalization (SB-A-NF). Titanium surfaces were characterized by surface roughness and scanning electron microscopy (SEM). Specimens were incubated with human saliva extracted from submandibular and sublingual glands. Total salivary protein adsorbed to titanium was quantified and samples were submitted to western blotting for mucin glycoprotein 2 (MG2) and lactoferrin identification.

Results

Surface roughness was statistically higher for SB-A and SB-A-NF groups. Scanning electron microscopy images confirmed that titanium surface treatments increased surface roughness with higher number of porous and scratches for SB-A and SB-A-NF groups. Total protein adsorption was significantly higher for SB-A and SB-A-NF groups (p < 0.05), which also presented higher interactions with MG2 and lactoferrin proteins.

Conclusion

The roughing of titanium surface by spot-blasting plus acid etching treatments contribute to higher interaction with salivary proteins, such as MG2 and lactoferrin.

Clinical significance

Titanium surface roughing increases the interactions of the substratum with salivary proteins, which can influence the integration of dental implants and their components to the oral environment. However, those treatments should be used carefully intraorally, avoiding increase biofilm formation.

How to cite this article

Cavalcanti YW, Soares RV, Assis MAL, Zenóbio EG, da Silva Girundi FM. Titanium Surface Roughing Treatments contribute to Higher Interaction with Salivary Proteins MG2 and Lactoferrin. J Contemp Dent Pract 2015;16(2):141-146.


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