The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 16 , ISSUE 3 ( March, 2015 ) > List of Articles

RESEARCH ARTICLE

Diaphonization: A Recipe to Study Teeth

Farhan Raza Khan, Kiran Rehman, Sadia Habib

Citation Information : Khan FR, Rehman K, Habib S. Diaphonization: A Recipe to Study Teeth. J Contemp Dent Pract 2015; 16 (3):248-251.

DOI: 10.5005/jp-journals-10024-1670

Published Online: 01-03-2015

Copyright Statement:  Copyright © 2015; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim

There are various techniques to study root canal morphology and diaphonization is one of them. There are various methods of decalcification and diaphonization, cited in literature and the main aim of this paper was to give a brief account of the various techniques and share our experience of the technique at a teaching institution in Karachi, Pakistan.

Materials and methods

Diaphonization is one of the oldest methods and is based on decalcification of teeth followed by clearing and dye penetration. The specimen is later studied under microscope without sectioning.

Results

After the process of clearing a three-dimensional (3D) structure of the internal canal anatomy was visible with naked eye.

Conclusion

This paper entails a detailed historical background as well as the author's technique including percentages of various chemicals used and the timing of immersion of teeth into these agents.

Clinical significance

The read out is simple and can be subjected to interpretation by direct observation under microscope and can be helpful for students undertaking research in not only the discipline of dentistry but also in other fields such as botany and zoology.

How to cite this article

Rehman K, Khan FR, Habib S. Diaphonization: A Recipe to Study Teeth. J Contemp Dent Pract 2015;16(3):248-251.


PDF Share
  1. Anatomie des Mundes: Braumüller und Seidel; 1842.
  2. Comparative evaluation of modified canal staining and clearing technique, conebeam computed tomography, peripheral quantitative computed tomography, spiral computed tomography, and plain and contrast medium-enhanced digital radiography in studying root canal morphology. J Endod 2010 Sep;36(9):1547-1551.
  3. Root canal morphology and variations of mandibular premolars by clearing technique: an in vitro study. J Contemp Dent Pract 2011 Jul-Aug;12(4):318-321.
  4. A clearing technique for the study of root canal systems. J Endod 1980 Jan;6(1):421-424.
  5. A preliminary analysis of the morphology of lateral canals after root canal filling using a tooth-clearing technique. Int Endod J 2003 Jan;36(1):54-63.
  6. Clearing Technique and its Potential Applications— An in Vitro Study. J Indian Dent Assoc 2011;5(11):1122.
  7. Mühlreiter's Anatomie des menschlichen Gebisses: A. Felix 1920.
  8. Descriptive anatomy of the human teeth: The SS White manufacturing co; 1902.
  9. The anatomy of the rootcanals of the teeth of the permanent dentition: William Wood; 1925.
  10. Endodontic therapy: Mosby; 1972.
  11. Anatomy of root canals: Tokyo Dental College; 1927.
  12. Decalcification of bone: Literature review and practical study of various decalcifying agents. Methods, and their effects on bone histology. J Histotechnol 1998;21(1):49-58.
  13. Root canal morphology of mandibular first and second premolars. Oral Surg Oral Med Oral Pathol 1973;36(5):738-744.
  14. Microleakage of human saliva through dentinal tubules exposed at the cervical level in teeth treated endodontically. J Endod 1996 Nov;22(11):579-582.
  15. Apical seal using the GPII method in straight canals compared with lateral condensation, with or without sealer. Oral Surg Oral Med Oral Pathol 1994 Aug;78(2):225-231.
  16. Evaluation of the apical seal produced by the McSpadden compactor and the lateral condensation with a chloroform-softened primary cone. J Endod 1983 May;9(5):190-197.
  17. Threaded endodontic dowels: effect of post design on incidence of root fracture. J Prosthet Dent 1991 Feb;65(2):179-187.
  18. Root canal morphology of mandibular premolars. J Am Dent Assoc 1978 Jul;97(1):47-50.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.