The Journal of Contemporary Dental Practice

Register      Login



Volume / Issue

Online First

Related articles

VOLUME 16 , ISSUE 5 ( May, 2015 ) > List of Articles


Antibacterial Potential of 2.5% Sodium Hypochlorite in Distinct Irrigation Protocols on Enterococcus faecalis Biofilm

Cyntia Rodrigues de Araújo Estrela, Ana Helena Gonçalves de Alencar, Carlos Estrela, Denise Ramos Silveira Alves, Rodrigo Sanches Cunha, Carlos Eduardo da Silveira Bueno, Tatiane Oliveira dos Santos

Citation Information : de Araújo Estrela CR, de Alencar AH, Estrela C, Alves DR, Cunha RS, da Silveira Bueno CE, Santos TO. Antibacterial Potential of 2.5% Sodium Hypochlorite in Distinct Irrigation Protocols on Enterococcus faecalis Biofilm. J Contemp Dent Pract 2015; 16 (5):340-346.

DOI: 10.5005/jp-journals-10024-1687

Published Online: 01-10-2015

Copyright Statement:  Copyright © 2015; The Author(s).



The aim of this study was to evaluate the effect of irrigation methods on antibacterial potential of 2.5% NaOCl on Enterococcus faecalis biofilm.

Materials and methods

Enterococcus faecalis biofilms were prepared during 60 days on 48 human root canals and randomized into control and experimental groups using positive and negative pressure irrigation. Bacterial growth was analyzed using turbidity of culture medium followed by UV spectrophotometry, and scanning electron microscopy (SEM) analyses were performed. Mean and standard deviations were used for evaluate the mean optical densities associated to the number of bacteria present culture, and Scheirer-Ray-Hare (an extension of the Kruskal-Wallis test) and Tamhane test to analyze the SEM images in the groups and thirds. Significance was set at 5%.


Enterococcus faecalis was still present after root canal cleaning regardless of irrigation methods or bacterial identification methods.


Positive and negative pressure irrigation protocols using 2.5% NaOCl show a similar capacity to reduce E. faecalis in infected root canals.

How to cite this article

Alves DRS, Cunha RS, da Silveira Bueno CE, de Alencar AHG, de Araújo Estrela CR, dos Santos TO, Estrela C. Antibacterial Potential of 2.5% Sodium Hypochlorite in Distinct Irrigation Protocols on Enterococcus faecalis Biofilm. J Contemp Dent Pract 2015;16(5):340-346.

PDF Share
  1. Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after ‘one-visit’ endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005;99(2):231-252.
  2. Hard-tissue debris accumulation analysis by high-resolution computed tomography scans. J Endod 2009;35(7):1044-1047.
  3. One-versus two-visit endodontic treatment of teeth with apical periodontitis: a histobacteriologic study. J Endod 2012;38(8):1040-1052.
  4. Dentinal tubule infection as the cause of recurrent disease and late endodontic treatment failure: a case report. J Endod 2012;38(2):250-254.
  5. Current challenges and concepts in the preparation of root canal systems: a review. J Endod 2004;30(8):559-567.
  6. Root canal irrigants. J Endod 2006;32(5):389-398.
  7. Control of microorganisms in vitro by endodontic irrigants. Braz Dent J 2003;14(3):187-192.
  8. A new solution for the removal of the smear layer. J Endod 2003;29(3):170-175.
  9. Mechanism of action of sodium hypochlorite. Braz Dent J 2002;13(2):113-117.
  10. Comparison of the in vivo antimicrobial effectiveness of sodium hypochlorite and chlorhexidine used as root canal irrigants: a molecular microbiology study. J Endod 2011;37(2):143-150.
  11. Antimicrobial efficacy of two irrigation techniques in tapered and non tapered canal preparations: an in vitro study. J Endod 2008;34(11):1374-1377.
  12. Root canal debridement using manual dynamic agitation or the EndoVac for final irrigation in a closed system and an open system. Int Endod J 2010;43(11):1001-1012.
  13. J Endod 2010;36(10):1664-1668.
  14. Comparison of the EndoVac system to needle irrigation of root canals. J Endod 2007;33(5):611-615.
  15. Comparison of the debridement efficacy of the EndoVac irrigation system and conventional needle root canal irrigation in vivo. J Endod 2010;36(11):1782-1785.
  16. Comparison of apical extrusion of NaOCl using the EndoVac or needle irrigation of root canals. J Endod 2010;36(2):338-341.
  17. Postoperative pain after the application of two different irrigation devices in a prospective randomized clinical trial. J Endod 2010;36(8):1295-1301.
  18. Cleaning efficacy of an apical negative-pressure irrigation system at different exposure times. Quintessence Int 2010;41(9):759-767.
  19. Comparison of the antimicrobial efficacy of irrigation using the EndoVac to endodontic needle delivery. J Endod 2010;36(3):509-511.
  20. A preliminary study of the antibacterial potential of cetylpyridinium chloride in root canals infected by E. faecalis. Braz Dent J 2012;23(6):645-653.
  21. Antimicrobial efficacy of ozonated water, gaseous ozone, sodium hypochlorite and chlorhexidine in infected human root canals. Int Endod J 2007;40(2):85-93.
  22. Antimicrobial effect of irrigant combinations within dentinal tubules. Int Endod J 1998;31(1):8-14.
  23. Efficacy of different irrigation and activation systems on the penetration of sodium hypochlorite into simulated lateral canals and up to working length: an in vitro study. J Endod 2010;36(7):1216-1221.
  24. Influence of irrigant needle depth in removing bioluminescent bacteria inoculated into instrumented root canals using real-time imaging in vitro. Int Endod J 2005;38(2):97-104.
  25. Penetration of sodium hypochlorite into dentin. J Endod 2010;36(5):793-796.
  26. Comparison of debris removal with three different irrigation techniques. J Endod 2011;37(9):1301-1305.
  27. Effect of apical preparation size and preparation taper on irrigant volume delivered by using negative pressure irrigation system. J Endod 2010;36(4):721-724.
  28. Comparative safety of various intracanal irrigation systems. J Endod 2009;35(4):545-549.
  29. In vivo efficacy of three different endodontic irrigation systems for irrigant delivery to working length of mesial canals of mandibular molars. J Endod 2012;38(4):445-448.
  30. Comparison of the effectiveness of three irrigation techniques in reducing intracanal Enterococcus faecalis populations: an in vitro study. J Endod 2009;35(10):1422-1427.
  31. Pyro sequencing analysis of the apical root canal microbiota. J Endod 2011;37(11):1499-1503.
  32. Biofilm formation of oral and endodontic Enterococcus faecalis. J Endod 2007;33(7):815-818.
  33. Effectiveness of three endodontic irrigants at various tubular depths in human dentin. J Endod 2001;27(3):206-208.
  34. The effect of exposure to irrigant solutions on apical dentin biofilms in vitro. J Endod 2006;32(5):434-437.
  35. Influence of an apical negative pressure irrigation system on bacterial elimination during endodontic therapy: a prospective randomized clinical study. J Endod 2012;38(9):1177-1181.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.