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VOLUME 16 , ISSUE 8 ( August, 2015 ) > List of Articles

RESEARCH ARTICLE

Antimicrobial Activity and pH of Calcium Hydroxide and Zinc Oxide Nanoparticles Intracanal Medication and Association with Chlorhexidine

Mario Tanomaru-Filho, Juliane M Guerreiro-Tanomaru, Gisele Faria, Alana Souza Aguiar, Renato Toledo Leonardo

Citation Information : Tanomaru-Filho M, Guerreiro-Tanomaru JM, Faria G, Aguiar AS, Leonardo RT. Antimicrobial Activity and pH of Calcium Hydroxide and Zinc Oxide Nanoparticles Intracanal Medication and Association with Chlorhexidine. J Contemp Dent Pract 2015; 16 (8):624-629.

DOI: 10.5005/jp-journals-10024-1732

Published Online: 01-08-2015

Copyright Statement:  Copyright © 2015; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim

To evaluate pH and antibacterial activity of pastes with calcium hydroxide [Ca(OH)2] and zinc oxide (ZnO) microparticles (micro) or nanoparticles (nano) and association with 0.4% chlorhexidine against Enterococcus faecalis.

Materials and methods

The following pastes were analyzed: Ca(OH)2/ZnO micro, (2) Ca(OH)2/ZnO nano, (3) Ca(OH)2/ ZnO micro + 0.4% chlorhexidine, (4) Ca(OH)2/ZnO nano + 0.4% chlorhexidine. Antibacterial activity against E. faecalis was evaluated by agar diffusion test. The direct contact test on planktonic cells of E. faecalis was performed for 30 and 60 seconds. Root canals from bovine teeth were filled with the pastes and pH was evaluated after 1, 7, 14, 21, 30 and 60 days. The data obtained were submitted to the statistical tests analysis of variance (ANOVA) and Tukey or Kruskal-Wallis and Dunn test, with a 5% significance level.

Results

Calcium hydroxide and zinc oxide nano, and the pastes with 0.4% chlorhexidine were more effective in agar diffusion test. In the direct contact test, the pastes with chlorhexidine showed the highest effect after 30 seconds. All pastes eliminated E. faecalis after 60 seconds. All pastes promoted an increase in pH. The highest increase in pH was observed with nanoparticle medications after 1 and 7 days (p < 0.05). After this period, the pastes presented similar pH increase.

Conclusion

It was concluded that calcium hydroxide and zinc oxide nanoparticles promoted greater initial alkalinization. The antimicrobial activity of the pastes against E. faecalis is favored by the association with chlorhexidine.

Clinical significance

Although nanoparticles of calcium hydroxide and zinc oxide promoted antibacterial effect, the activity against E. faecalis is favored by association with chlorhexidine.

How to cite this article

Aguiar AS, Guerreiro-Tanomaru JM, Faria G, Leonardo RT, Tanomaru-Filho M. Antimicrobial Activity and pH of Calcium Hydroxide and Zinc Oxide Nanoparticles Intracanal Medication and Association with Chlorhexidine. J Contemp Dent Pract 2015;16(8):624-629.


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  1. Relationship between calcium hydroxide pH levels in the root canals and periodontal healing after replantation of avulsed teeth. Endod Top 2006;14(1):93-101.
  2. Ca(OH)2 application modes: in vitro alkalinity and clinical effect on bacteria. Intl Endod J 2014;47(7):628-638.
  3. Influence of dentin on pH of 2% chlorhexidine gel and calcium hydroxide alone or in combination. Dent Traumatol 2010;26(3):276-280.
  4. pH and calcium ion release evaluation of pure and calcium hydroxide-containing Epiphany for use in retrograde filling. J Appl Oral Sci 2011;19(1):1-5.
  5. Endodontic re-instrumentation enhances hydroxyl ion diffusion through radicular dentine. Int Endod J 2014;47(8):776-783.
  6. An in vitro study of the pH of three calcium hydroxide dressing materials. Dent Traumat 2007;23(1):21-25.
  7. The effects of sodium hypochlorite and calcium hydroxide on tissue dissolution and root canal cleanliness. Int Endod J 1997;30(5):335-342.
  8. Calcium ion release from calcium hydroxide stimulated fibronectin gene expression in dental pulp cells and the differentiation of dental pulp cells to mineralized tissue forming cells by fibronectin. Int Endod J 2008;41(11):933-938.
  9. Effect of different irrigation solutions and calcium hydroxide on bacterial LPS. Int Endod J 2003;36(11):733-739.
  10. Comparative analysis of endodontic pathogens using checkerboard hybridization in relation to culture. Oral Microbiol Immunol 2008;23(4):282-290.
  11. Molecular identification and quantification of bacteria from endodontic infections using real-time polymerase chain reaction. Oral Microbiol Immunol 2008;23(5):384-390.
  12. Antimicrobial effects of four intracanal medicaments on enterococcus faecalis: an in vitro study. Iran Endod J 2014;9(3):195-198.
  13. Clonal diversity in biofilm formation by Enterococcus faecalis in response to environmental stress associated with endodontic irrigants and medicaments. Intl Endod J 2015 Mar;48(3):210-219.
  14. Karygianni L, Altenburger MJ, Pelz K, Hellwig E, Al-Ahmad A. New bacterial composition in primary and persistent/secondary endodontic infections with respect to clinical and radiographic findings. J Endod 2014;40(5):670-677.
  15. Lipoteichoic acid partially contributes to the inflammatory responses to Enterococcus faecalis. J Endod 2008;34(8):975-982.
  16. Chlorhexidine gluconate attenuates the ability of lipoteichoic acid from Enterococcus faecalis to stimulate toll-like receptor 2. J Endod 2009;35(2):212-215.
  17. Effectiveness of calcium hydroxide-based intracanal medicaments against Enterococcus faecalis. Int Endod J 2012;45(4):311-316.
  18. Microbial evaluation of traumatized teeth treated with triple antibiotic paste orcalcium hydroxide with 2% chlorhexidine gel in pulp revascularization. J Endod 2014;40(6):778-783.
  19. Radiopacity evaluation of calcium aluminate cement containing different radiopacifying agents. J Endod 2011;37(1):67-71.
  20. Evaluation of the radiopacity of calcium silicate cements containing different radiopacifiers. Int Endod J 2010;43(1):21-30.
  21. Nanotechnology as a therapeutic tool to combat microbial resistance. Adv Drug Deliv Rev 2013; 65(13-14):1803-1815.
  22. An investigation on the antibacterial and antibiofilm efficacy of cationic nanoparticulates for root canal disinfection. J Endod 2008;34(12):1515-1520.
  23. Nanoparticulates for antibiofilm treatment and effect of aging on its antibacterial activity. J Endod 2010;36(6):1030-1035.
  24. Antifungal coatings based on Ca(OH)2 mixed with ZnO/TiO2 nanomaterials for protection of limestone monuments. ACS Appl Mater Interfaces 2013;5(5):1556-1565.
  25. Sizedependent antimicrobial response of zinc oxide nanoparticles. IET Nanobiotechnol 2014;8(2):111-117.
  26. Antimicrobial activity of zinc oxide (ZnO) nanoparticle against Klebsiella pneumoniae. Pharm Biol 2014;52(11):1388-1397.
  27. Vehicle influence on calcium hydroxide pastes diffusion in human and bovine teeth. Dent Traumatol 2006;22(6):302-306.
  28. Inactivation of local root canal medicaments by dentine: an in vitro study. Int Endod J 2000;33(2):126-131.
  29. An in vitro comparison of pH changes in root dentine following canal dressing with calcium hydroxide points and a conventional calcium hydroxide paste. Intl Endod J 2002;35(3):239-244.
  30. Radio-opaque bioactive glass for potential root canal applicaction: evaluation of radiopacity, bioactivity and alkaline capacity. Int Endod J 2010;43(3):210-217.
  31. Particle size and shape of calcium hydroxide. J Endod 2009;35(2):284-287.
  32. Evaluation of pH and calcium ion release of calcium hydroxide pastes containing different substances. J Endod 2009;35(9):1274-1277.
  33. Release and diffusion of hydroxyl ion from calcium hydroxide-based medicaments. Dent Traumatol 2012;28(4):320-323.
  34. Assessment of diffusion of hydroxyl and calcium ions of root canal filling materials in primary teeth. Pediatr Dent 2012;34(2):122-126.
  35. Intracanal placement of calcium hydroxide: a comparison of specially designed paste carrier technique with other techniques. BMC Oral Health 2013;13:52.
  36. Effect of photodynamic therapy (PDT) on Enterococcus faecalis biofilm in experimental primary and secondary endodontic infections. BMC Oral Health 2014;14(1):132.
  37. Detection of Enterococcus faecalis and Candida albicans in previously root-filled teeth in a population of Gujarat with polymerase chain reaction. Contemp Clin Dent 2013;4(1):62-66.
  38. Impact of growth conditions on susceptibility of five microbial species to alkaline stress. J Endod 2008;34(5):579-582.
  39. Survival of Enterococcus faecalis in root canals ex vivo. Int Endod J 2005;38(10):735-742.
  40. Prevalence of putative virulence factors and antimicrobial susceptibility of Enterococcus faecalis isolates from patients with dental Diseases. BMC Oral Health 2008;8(1):17.
  41. Is chlorhexidine an ideal vehicle for calcium hydroxide? A microbiologic review. Iran Endod J 2012;7(3):115-122.
  42. In vitro antibacterial effect of calcium hydroxide combined with chlorhexidine or iodine potassium iodide on Enterococcus faecalis. Eur J Oral Sci 2004;112(4):326-331.
  43. Antimicrobial efficacy of octenidine hydrochloride, MTAD and chlorhexidine gluconate mixed with calcium hydroxide. J Contemp Dent Pract 2013 May 1;14(3):456-460.
  44. Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide. Int Endod J 2002;35(3):221-228.
  45. Antimicrobial activity of short- and medium-term applications of polyhexamethylene biguanide, chlorhexidine digluconate and calcium hydroxide in infected immature bovine teeth in vitro. Dent Traumatol 2014;30(4):326-331.
  46. Antimicrobial effect of conventional root canal medicaments vs propolis against Enterococcus faecalis, Staphylococcus aureus and Candida albicans. J Contemp Dent Pract 2012;13(3):305-309.
  47. The properties and applications of chlorhexidine in endodontics. Int Endod J 2009;42(4):288-302.
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