Biocorrosion of Endodontic Files through the Action of Two Species of Sulfate-reducing Bacteria: Desulfovibrio desulfuricans and Desulfovibrio fairfieldensis
Fabiano Luiz Heggendorn, Lucio Souza Gonçalves, Eliane Pedra Dias, Viviane de Oliveira Freitas Lione, Márcia Teresa Soares Lutterbach
Citation Information :
Heggendorn FL, Gonçalves LS, Dias EP, Lione VD, Lutterbach MT. Biocorrosion of Endodontic Files through the Action of Two Species of Sulfate-reducing Bacteria: Desulfovibrio desulfuricans and Desulfovibrio fairfieldensis. J Contemp Dent Pract 2015; 16 (8):665-673.
This study assessed the biocorrosive capacity of two bacteria: Desulfovibrio desulfuricans and Desulfovibrio fairfieldensis on endodontic files, as a preliminary step in the development of a biopharmaceutical, to facilitate the removal of endodontic file fragments from root canals.
Materials and methods
In the first stage, the corrosive potential of the artificial saliva medium (ASM), modified Postgate E medium (MPEM), 2.5 % sodium hypochlorite (NaOCl) solution and white medium (WM), without the inoculation of bacteria was assessed by immersion assays. In the second stage, test samples were inoculated with the two species of sulphur-reducing bacteria (SRB) on ASM and modified artificial saliva medium (MASM). In the third stage, test samples were inoculated with the same species on MPEM, ASM and MASM. All test samples were viewed under an infinite focus Alicona microscope.
Results
No test sample became corroded when immersed only in media, without bacteria. With the exception of one test sample between those inoculated with bacteria in ASM and MASM, there was no evidence of corrosion. Fifty percent of the test samples demonstrated a greater intensity of biocorrosion when compared with the initial assays.
Conclusion
Desulfovibrio desulfuricans and D. fairfieldensis are capable of promoting biocorrosion of the steel constituent of endodontic files.
Clinical significance
This study describes the initial development of a biopharmaceutical to facilitate the removal of endodontic file fragments from root canals, which can be successfully implicated in endodontic therapy in order to avoiding parendodontic surgery or even tooth loss in such events.
How to cite this article
Heggendorn FL, Gonçalves LS, Dias EP, de Oliveira Freitas Lione V, Lutterbach MTS. Biocorrosion of Endodontic Files through the Action of Two Species of Sulfatereducing Bacteria: Desulfovibrio desulfuricans and Desulfovibrio fairfieldensis. J Contemp Dent Pract 2015;16(8):665-673.
New approach for the retrieval of broken instruments. J Endod 1999;25(9):633-635.
Remoção de instrumento endodôntico fraturado no interior do canal radicular. Caso clínico [Endodontic instrument removal fractured inside the root canal. Clinical case]. J Bras Endod 2003;4(14):186-190. (Port).
Probability of removing fractured instruments from root canals. Int Endod J 2005;38(2):112-123.
The causes, prevention, and clinical management of broken endodontic rotary files. Dent Today 2005;24(11):74,76.78-80.
Removal of separated files from root canals with a new file-removal system: case reports. J Endod 2006;32(8):789-797.
Corrosão em limas endodônticas de aço inoxidável [Corrosion of stainless steel endodontic files]. Rev Bras Odontol 1994;51(1):26-28. (Port).
Análise Morfométrica da Corrosão de três instrumentos rotatórios de níquel-titânio [Morphometric analysis of corrosion of three nickel-titanium rotary instruments]. Ecler Endod 2000;2(1).(Port).
Corrosion rates of stainless-steel files in different irrigating solutions. Int Endod J 2002;35(8):655-659.
Biocorrosão, Biofouling e Biodeterioração de materiais [Biocorrosion, Biofouling and Biodeterioration materials]. 1st ed. São Paulo: Edgard Biucher; 2003. 148 p. (Port).
Isolation and characterization of Desulfovibrio growing on hydrogen plus sulfate as the sole energy source. Arch Microbiol 1978;116(1):41-49.
The Sulphate-reducing bacteria. 2nd ed. London: Cambridge University Press; 1984.
Corrosão [Corrosion]. 3rd ed. Rio de Janeiro: LTC; 1996.
Biología de los Microorganismos [Biology of Microorganisms], 10th ed. Madrid: Pearson-Prentice Hall, 2003. 1011p. (Spa).
Geomicrobiology. 5th ed. New York: CRC Press; 2009.
Frist isolation of Desulfovibrio species as part of a polymicrobial infection from a brain abscess. Eur J Clin Microbiol Infect Dis 1999;18(8):602-603.
Desulfovibrio desulfuricans Bacteremia in a dog. J Clin Microbiol 2000;38(4):1701-1702.
About a synthetic saliva for in vitro studies. Talanta 2001;53(6):1103-1115.
Handbook of Biomaterial Properties. 1st ed. New York: Springer Science and Business Media; 1998. 590 p.
A role for sulphate reducing bacteria in ulcerative colitis? Gastroenterology 1990;98:A170.
Activity of sulfate-reducing bacteria in human periodontal pocket. Can J Microbiol 2002;48(12):1099-1103.
Sulphate-reducing Bacteria Environmental and engineered systems. 1st ed. New York: Cambridge University Press; 2007.
Interaction of Desulfovibrio desulfuricans biofilms with stainless steel surface and its impact on bacterial metabolism. J appl Microbiol 2006;101(5):1087-1095.
Encyclopedia of Microbiology. 3rd ed. New York: Elsevier Academic Press; 2009. 4600 p.
Growth and morphology of thick film formed on a metallic surface. Electrochimica Acta 1996;41(11):1855-1862.
Biomaterials: An Introduction. 3rd ed. New York: Springer, 2007. 394 p.
A cleaning protocol for rotary nickel-titanium endodontic. Aust Dent J 2004;49(1):20-27.
J Clin Periodontol 2000;27(12):943-950.
Avaliação da desinfecção do canal radicular frente ao preparo químico-cirúrgico por meio rotatório associado ou não a tratamento químico complementar [Evaluation of disinfecting root canal front of the chemicalsurgical preparation through associated rotary or not the additional chemical treatment] [Dissertation]. São Paulo: Faculdade de Odontologia da Universidade de São Paulo [Faculty of Dentistry, University of São Paulo]; 2008.
Microbiologia do insucesso do tratamento endodôntico [Microbiology of failure of endodontic treatment]. Rev Gestão Saúde 2009;1(1):11-17. (Port).
