Apical Extrusion of Intracanal Bacteria following use of Two Engine-driven Instrumentation Techniques: An in vitro Study
Niyaz Mohammed, MC Noushad, Biji Balan, N Dhanesh, Nishi Jayasheelan, Vanita D Revankar
Citation Information :
Mohammed N, Noushad M, Balan B, Dhanesh N, Jayasheelan N, Revankar VD. Apical Extrusion of Intracanal Bacteria following use of Two Engine-driven Instrumentation Techniques: An in vitro Study. J Contemp Dent Pract 2016; 17 (11):939-942.
The aim of the present study was to compare in vitro the amount of debris extruded apically from extracted teeth, using Twisted files and ProTaper rotary during two different instrumentation systems.
Materials and methods
Forty-five human single-rooted mandibular premolar teeth were randomly divided into three groups and contaminated with Enterococcus faecalis. The teeth in experimental groups were instrumented until reaching the working length with ProTaper rotary instruments and Twisted files with XSmart and XSmart Dual groups. Debris extruded from the apical foramen was collected into glass vials and the amount of bacteria was calculated. The data obtained were analyzed using Kruskal–Wallis one-way analysis of variance and Mann–Whitney U tests, with p = 0.05 as the level for statistical significance.
The XSmart Dual group extruded comparatively lesser bacteria compared to the XSmart group. Lesser amount of bacterial extrusion was seen when Twisted files were used compared to the ProTaper files (p < 0.05).
Under the circumstances of this in vitro study, it can be concluded that all instrumentation techniques produced measurable apical extrusion of debris. So, it is upon the practitioner to decide which system best fits their individual needs and their level of skill and experience that will provide the best possible endodontic care for our patients.
The newer instrument designs, including noncutting tips, different cross sections, radial lands, and variable tapers, are better for the clinicians to improve working safety, to reduce the working time, and to create a greater flare within the preparations.
How to cite this article
Mohammed N, Noushad MC, Balan B, Dhanesh N, Jayasheelan N, Revankar VD. Apical Extrusion of Intracanal Bacteria following use of Two Engine-driven Instrumentation Techniques: An in vitro Study. J Contemp Dent Pract 2016;17(11):939-942.
Apical extrusion of intracanal bacteria following use of two engine-driven instrumentation techniques. Int Endod J 2005 Dec;38:871-876.
Apical extrusion of debris and irrigant using hand and rotary systems: a comparative study. J Conserv Dent 2011 Apr-Jun;14(2):187-190.
Microbial causes of endodontic flare-ups. Int Endod J 2003 Jul;36(7):453-463.
An in vitro comparative study of apically extruded debris resulting from conventional and three rotary (Profile, Race, FlexMaster) instrumentation techniques. J Oral Sci 2006 Jun;48(2):85-88.
In vitro evaluation of the accuracy of five different electronic apex locators. Saudi Endod J 2015 Sep-Dec;5(3):177-181.
A quantitative assessment of canal debris forced periapically during root canal instrumentation using two different techniques. J Endod 1987 Dec;13(12):554-558.
Effect of irrigation on the production of extruded material at the root apex during instrumentation. J Endod 1975 Jul;1(7):243-246.
An in vivo evaluation of the penetration of an irrigating solution in root canals. J Endod 1977 Oct;3(10):394-398.
An in vitro comparison of apically extruded debris using three rotary nickel-titanium instruments. J Dent Sci 2010 Sep;5(3):121-125.
The presence and distribution of microorganisms within nonvital teeth. Br Dent J 1964 Aug;117(3):101-107.
Instrumentation of root canals in molars using the step-down technique. J Endod 1982 Dec;8(12):550-554.
To evaluate the effect of two passive ultrasonic irrigation methods on removal of dentin debris from root canal systems using computational fluid dynamics study model. Int J Contemp Dent Med Rev 2014;2014:011214. doi:10.15713/ins.ijcdmr.20.
Microbiology of apical periodontitis. In:Orstavik D, Pitt Ford TR, editors. Essential endodontology: prevention and treatment of apical periodontitis. 1st edn. Oxford: Blackwell Sciences Ltd; 1998. p. 106-125.
Enterococcus faecalis: a resistant microbe in endodontics. Int J Contemp Dent Med Rev 2014;2014:011114. doi:10.15713/ins.ijcdmr.5