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VOLUME 17 , ISSUE 2 ( February, 2016 ) > List of Articles

RESEARCH ARTICLE

Action of Chlorhexidine, Zingiber officinale, and Calcium Hydroxide on Candida albicans, Enterococcus faecalis, Escherichia coli, and Endotoxin in the Root Canals

Marcia C Valera, Sarah AC Oliveira, Lilian E Maekawa, Flávia GR Cardoso, Adriana Chung, Stephanie FP Silva, Cláudio AT Carvalho

Citation Information : Valera MC, Oliveira SA, Maekawa LE, Cardoso FG, Chung A, Silva SF, Carvalho CA. Action of Chlorhexidine, Zingiber officinale, and Calcium Hydroxide on Candida albicans, Enterococcus faecalis, Escherichia coli, and Endotoxin in the Root Canals. J Contemp Dent Pract 2016; 17 (2):114-118.

DOI: 10.5005/jp-journals-10024-1812

Published Online: 01-02-2016

Copyright Statement:  Copyright © 2016; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim

The purpose of this in vitro study was to evaluate the antimicrobial activity of 2% chlorhexidine gel (CHX) as auxiliary chemical substance and intracanal medications on Candida albicans, Candida albicans, Enterococcus faecalis, Escherichia coli, and their endotoxins in the root canals.

Materials and methods

The study was conducted on 48 single-rooted human teeth divided into four groups (n = 12), according to intracanal medications used: (1) Calcium hydroxide + apyrogenic saline solution (Ca(OH)2 + SS), (2) 20% ginger glycolic extract (GEN), (3) calcium hydroxide + 20% ginger glycolic extract (Ca(OH)2 + GEN), (4) apyrogenic SS (control). Collections were made from the root canal content before preparation (baseline–S1), immediately after instrumentation (S2), 7 days after instrumentation (S3), after 14 days the action of intracanal medication (S4), and 7 days after removal of the intracanal medication (S5). The antimicrobial activity and endotoxin content were analyzed for all collections. The results were statistically analyzed by the Kruskal–Wallis and Dunn tests at a significance level of 5%.

Results

After instrumentation with CHX, there was complete elimination of E. coli and C. albicans, except for E. faecalis, which was significantly reduced and then completely eliminated after intracanal medication. There was significant reduction of endotoxin after instrumentation. Comparison of collection after instrumentation and intracanal medication revealed reduction of endotoxins in all groups; this reduction was greater in group Ca(OH)2 followed by the group GEN.

Conclusion

It was concluded that the instrumentation using CHX and intracanal medication used were able to eliminate the microorganisms from the root canal; the endotoxins were reduced, yet not completely eliminated.

Clinical significance

This study is important and relevant for searching alternatives during endodontic therapy, since it aims to study the effect of Zingiber officinale on microorganisms and endotoxins present in root canals.

How to cite this article

Valera MC, Oliveira SAC, Maekawa LE, Cardoso FGR, Chung A, Silva SFP, Carvalho CAT. Action of Chlorhexidine, Zingiber officinale, and Calcium Hydroxide on Candida albicans, Candida albicans, Enterococcus faecalis, Escherichia coli, and Endotoxin in the Root Canals. J Contemp Dent Pract 2016; 17(2):114-118.


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  1. Morphometrical analysis of cleaning capacity using nickel-titanium rotary instrumentation associated with irrigating solutions in mesio-distal flattened root canals. J Appl Oral Sci 2003 Mar;11(1):55-59.
  2. Candida in root canal in accordance with oral ecology. Int Endod J 1992;25:40.
  3. Microbiologic analysis of teeth failed endodontic treatment and the outcome of conservative retreatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998 Jan;85(1):86-93.
  4. Enterococcus faecalis —a mechanism for its role in endodontic failure. Int Endod J 2001 Jul;34(5):399-405.
  5. Microbiota of periapical lesions refractory to endodontic therapy. J Endod 2002 Apr;28(4):304-310.
  6. Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004 Jan;97(1):85-94.
  7. Diffusion ability of endotoxin through dentinal tubules. Braz Oral Res 2005 Jan-Mar;19(1):5-10.
  8. Efficacy of chlorhexidine in disinfecting dentinal tubules in vitro. Endod Dent Traumatol 1993 Dec;9(6):243-248.
  9. In vitro evaluation of the effectiveness of irrigants and intracanal medicaments on microorganisms within root canals. Int Endod J 2004 May;37(5):311-319.
  10. In vitro antimicrobial activity of sodium hypochlorite and chlorhexidine against selected single-species biofilms. Int Endod J 2006 Nov;39(11):878-885.
  11. Chlorhexidine in endodontics. Braz Dent J 2013;24(2):89-102.
  12. In vitro evalution of the actions of irrigating solutions associated with intracanal medications on Escherichia coli and its endotoxin in root canals. J Appl Oral Sci 2011 Apr;19(2):106-112.
  13. Action of propolis and medications against Escherichia coli and endotoxin in root canals. Oral Surg, Oral Med, Oral Pathol, Oral Radiol Endod 2010 Oct;110(4):e70-e74.
  14. Bacterial reduction and persistence after endodontic treatment procedures. Oral Microbiol Immunol 2007 Feb;22(1):19-23.
  15. Antimicrobial action of Zingiber officinale front of the oral microbiota. Rev Est Biol 2006;28:61-66.
  16. Pathogenesis of apical periodontitis and the causes of endodontic failures. Crit Rev Oral Biol Med 2004 Nov 1;15(6):348-381.
  17. Bacteria in the apical root canal of teeth with primary apical periodontitis. Oral Surg, Oral Med, Oral Pathol, Oral Radiol Endod 2009 May;107(5):721-726.
  18. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 2006 Feb;32(2):93-98.
  19. Fungi in therapy-resistant apical periodontitis. Int Endod J 1997 Mar;30(2):96-101.
  20. Microbiological examination of infected dental root canals. Oral Microbiol Immunol 2004 Apr;19(2):71-76.
  21. Microbiological eveluation of acute periradicular abscesses by DNA-DNA hybridization. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001 Oct;92(4):451-457.
  22. Radiographic evaluation of the effect of endotoxin (LPS) plus calcium hydroxide on apical and periapical tissues of dogs. J Endod 2002 Oct;28(10):694-696.
  23. Effectiveness of 2% chlorhexidine gel and calcium hydroxide against Enterococcus faecalis in bovine root dentine in vitro. Int Endod J 2003 Apr;36(4):267-275.
  24. The antimicrobial effect of calcium hydroxide as a short-term intracanal dressing. Int Endod J 1991 May;24(3):119-125.
  25. Mechanisms of antimicrobial activity of calcium hydroxide: a critical review. Int Endod J 1999 Sep;32(5):361-369.
  26. Calcium hydroxide root canal dressing. Histopathological evaluation of periapical repair at different time periods. Braz Dent J 2002;13(1):17-22.
  27. Importance of bacterial endotoxin (LPS) in endodontics. J Appl Oral Sci 2004 Jun;12(2):93-98.
  28. Study of the effect of ginger on the healing of ulcers in the oral mucosa of rats. Pesqui Odontol Bras 2003;17:76.
  29. Zingiberis rhizoma: a comprehensive review on the ginger effect and efficacy profiles. Phytomedicine 2005 Sep;12(9):684-701.
  30. Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): a review of recent research. Food Chem Toxicol 2008 Feb;46(2):409-420.
  31. The effect of extracts from ginger rhizome on inflammatory mediator production. Phytomedicine 2007 Feb;14(2–3):123-128.
  32. Hexane fraction of zingiberis rhizoma crudus extract inhibits the production of nitric oxide and proinflammatory cytokines in LPS-stimulated BV2 microglial cells via the NF-KappaB pathway. Food Chem Toxicol 2009 Jun;47(6):1190-1197.
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