The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 17 , ISSUE 3 ( March, 2016 ) > List of Articles

RESEARCH ARTICLE

Evaluation of Antimicrobial Photodynamic Therapy against Streptococcus mutans Biofilm in situ

VH Panhóca, FLE Florez, N Batista de Faria Júnior, Alessandra Nara de Souza Rastelli, JMG Tanomaru, C Kurachi, VS Bagnato

Citation Information : Panhóca V, Florez F, de Faria Júnior NB, de Souza Rastelli AN, Tanomaru J, Kurachi C, Bagnato V. Evaluation of Antimicrobial Photodynamic Therapy against Streptococcus mutans Biofilm in situ. J Contemp Dent Pract 2016; 17 (3):184-191.

DOI: 10.5005/jp-journals-10024-1825

Published Online: 01-03-2016

Copyright Statement:  Copyright © 2016; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim

This study investigated the effect of antimicrobial photodynamic therapy (aPDT) over Streptococcus mutans biofilm.

Materials and methods

Eighteen (n = 18) patients were selected and one palatine device with dental blocks was used. The biofilm was treated by curcumin and Photogem® with a LED and the effect was analyzed by CFU/ml.

Results

Although, statistical analysis showed significant reductions for aPDT mainly with Photogem® (p = 0.02), these were low.

Conclusion

The results suggest a low antimicrobial effect of aPDT over S. mutans biofilm. Some parameters used need to be improved.

Clinical Significance

This technique can be a promising in Dentistry.

How to cite this article

Panhóca VH, Florez FLE, Batista de Faria N Jr, Rastelli ANS, Tanomaru JMG, Kurachi C, Bagnato VS. Evaluation of Antimicrobial Photodynamic Therapy against Streptococcus mutans Biofilm in situ. J Contemp Dent Pract 2016;17(3):184-191.


PDF Share
  1. Dental caries. Lancet 2007;369:51-59.
  2. Bacterial biofilms and human disease. Sci Prog 2001;84(3):235-254.
  3. Dental plaque revisited: oral biofilms in health and disease. J Periodont Res 1998;33(7):438.
  4. Photodynamic antimicrobial chemotherapy (PACT). J Antimicrob Chemoter 1998;42(1):13-28.
  5. The continuum of dental caries – evidence for a dynamic disease process. J Dent Res 2004;83C:C39-C42.
  6. Pathophysiology: concepts of altered health states. 3rd ed. Philadelphia, PA: Lippincott; 1990.
  7. Principles and practice of adult health nursing. St Louis, MO: Mosby; 1990.
  8. Antimicrobial photodynamic therapy with two photosensitizers on two oral streptococci: an in vitro study. Laser Phys 2011;21(12):2132-2137.
  9. Light parameters influence cell viability in antifungal photodynamic therapy in a fluence and rate fluencedependent manner. Laser Phys 2009;19(5):1038-1044.
  10. Methylene blue mediated laser therapy of maxillary sinusitis. Laser Phys 2006;16(7):1128-1133.
  11. Nanodrug applications in photodynamic therapy. Photodiagnosis Photodyn Ther 2011;8:14-29.
  12. Photodynamic therapy: a new antimicrobial approach to infectious disease? Photochem Photobiol Sci 2004;3:436-450.
  13. Photodynamic therapy of microbial infections: state of the art an perspective. J Envirom Pathol Toxicol Oncol 2006;25(1):505-519.
  14. Photodynamic therapy for localized infections-state of the art. Photodiagnosis Photodyn Ther 2009;6(3-4):170-188.
  15. Overall- Mouth disinfection by photodynamic therapy using curcumin. Photomed Laser Surg 2012;30(2):96-101.
  16. Antimicrobial photodynamic action on dentin using a light-emitting diode light source. Photomed Laser Surg 2008;26:279-285.
  17. Susceptibility of Streptococcus mutans biofilms to photodynamic therapy: an in vitro study. J Antimicrob Chemother 2005;56:324-330.
  18. Photosensitization of in vitro biofilms by toluidine blue O combined with a light-emitting diode. Eur J Oral Sci 2006;114:64-69.
  19. Mesosubstituted cationic porphyrins as efficient photosensitizers of Gram-positive and Gram-negative bacteria. J Photochem Photobiol 1996;B 32:153-157.
  20. Photolysis of oral bacteria and its potential use in the treatment of caries and periodontal disease. J Appl Bacteriol 1993;75:299-306.
  21. The modes of cell death induced by PDT: an overview. Med Laser Appl 2003;18:7-19.
  22. Sensitization of cariogenic bacteria to killing by light from helium-neon laser. J Med Microbiol 1993;38(6):401-405.
  23. Sensitization of oral bacteria in biofilms to killing by light from low-power laser. Arch Oral Biol 1992;37(11):883-887.
  24. Dye-mediated bactericidal effect of He-Ne laser irradiation on oral microorganisms. Lasers Surg Med 1992;12(4):450-458.
  25. Photodynamic therapy: a new concept in medical treatment. Braz J Med Biol Res 2000;33(8):869-880.
  26. Phototherapy of cancer and atheromatous plaque with texaphyrins. J Clin Laser Med Surg 1996;14(5):343-348.
  27. Photodynamic therapy: a new antimicrobial approach to infectious disease? Photochem Photobiol Sci 2004;3:436-450.
  28. The physics of photodynamic therapy. Phys Med Biol 1986;31:327-360.
  29. Antifungal photodynamic therapy. Microbiol Res 2008;163:1-12.
  30. Photodynamic therapy for periodontal diseases: state of the art. J Photochem Photobiol B 2005;79:159-170.
  31. Curcumin: the Indian solid gold. Adv Exp Med Biol 2007;595:1-75.
  32. Evaluation of the antimicrobial effect of photodynamic antimicrobial therapy in an in situ model of dentine caries. Eur J Oral Sci 2009;117:568-574.
  33. In vitro photodynamic antimicrobial chemotherapy in dentine contaminated by cariogenic bacteria. Laser Phys 2010;20:1-10.
  34. Photodynamic effect of light-emitting diode light on cell growth inhibition induced by methylene blue. J Bio Sci 2008;33:231-237.
  35. Bactericidal effect of malachite green and red laser on Actinobacillus actinomycetemcomitans. J Photochem Photobiol 2007;B 86: 70-76.
  36. Enhancement of erythrosine mediated photodynamic therapy of Streptococcus mutans biofilms by light fractionation. J Antimicrobial Chemother 2006;58:190-192.
  37. Photodynamic antimicrobial chemotherapy by liposome-encapsulated watersoluble photosensitizers. Bioorg Khim 2010;6:396-402.
  38. Effect of saliva processing on bacterial DNA extraction. New Microbiol 2010;33:373-379.
  39. Susceptibility of clinical isolates of Candida to photodynamic effects of curcumin. Lasers Surg Med 2011;43:927-934.
  40. Photokilling of bacteria by curcumin in selected polyethylene glycol 400 (PEG 400) preparations. Studies on curcumin and curcuminoids. XLI Pharmazie 2010;65:600-606.
  41. Phototoxic effect of curcumin on methicillin-resistant Staphylococcus aureus and L929 fibroblasts. Lasers Med Sci 2013;28(2):391-398.
  42. Biochemical composition and cariogenicity of dental plaque formed in the presence of sucrose or glucose and fructose. Caries Res 2000;34:491-497.
  43. Effect of sucrose concentration on dental biofilm formed in situ and on enamel demineralization. Caries Res 2006;40:28-32.
  44. A selective medium for Streptococcus mutans. Arch Oral Biol 1973;18:1357-1364.
  45. Clinical decision-making for coronal caries management in the permanent dentition. J Dent Educ 2001;65:1143-1146.
  46. The cariogenic dental biofilm: good, bad or just something to control? Braz Oral Res 2009;23:31-38.
  47. Photodynamic therapy in dentistry. J Dent Res 2007;86:694-707.
  48. Photodynamic therapy disinfection of carious tissue mediated by aluminum-chloridephthalocyanine entrapped in cationic liposomes: an in vitro and clinical study. Lasers Med Sci 2012;27:575-584.
  49. Photophysics, photochemistry and photobiology of curcumin: studies from organic solutions, biomimetics and living cells. J Photoch Photobio C 2009;10:81-95.
  50. Photokilling of bacteria by the natural dye curcumin. Arch Microbiol 1989;151:183-185.
  51. Studies on curcumin and curcuminoids. IX: Investigation of the photobiological activity of curcumin using bacterial indicator systems. J Pharm Sci 1987;76:371-373.
  52. The effect of variable energy input from a novel light source on the photoactivated bactericidal action of toluidine blue O on Streptococcus mutans. Caries Res 2003;37:190-193.
  53. Effects of growth phase and extracellular slime on photodynamic inactivation of Gram-positive pathogenic bacteria. Antimicrob Agents Chemother 2004;48(6):2173-2178.
  54. Analysis of biocide transport limitation in an artificial biofilm system. J Appl Microbiol 1998;85:495-500.
  55. Diffusion in biofilms. J Bacteriol 2003;185:1485-1491.
  56. Rapid diffusion of fluorescent tracers into Staphylococcus epidermidis biofilms visualized by time lapse microscopy. Antimicrob Agents Chemother 2005;49:728-732.
  57. Oral bacteria in multi-species biofilms can be killed by red light in the presence of toluidine blue. Lasers Surg Med 2002;3:86-90.
  58. Bacterial biofilms: a common cause of persistent infections. Sci 1999;284:1318-1322.
  59. Direct measurement of chlorine penetration into biofilms during disinfection. Appl Environ Microbiol 1994;60(12):4339-4344.
  60. Photodestruction of human dental plaque bacteria: enhancement of the photodynamic effect by photomechanical waves in oral biofilm model. Lasers Surg Med 2003;33(3):161-168.
  61. The antibacterial effect of photodynamic therapy in dental plaque-derived biofilms. J Periodontal Res 2009;44(6):751-759.
  62. Bacteria in supragingival plaque samples can be killed by low-power laser light in the presence of a photosensitizer. J Appl Bacteriol 1995;78:569-574.
  63. Lethal photosensitization of oral bacteria and its potential application in the photodynamic therapy of oral infections. Photochem Photobiol Sci 2004;3:412-418.
  64. Photolysis of oral bacteria and its potential use in the treatment of caries and periodontal disease. J Appl Bacteriol 1993;75:299-306.
  65. Photokilling of bacteria by curcumin in different aqueous preparations. Studies on curcumin and curcuminoids XXXVII. Pharmazie 2009;64(10):666-673.
  66. Comparison of the methylene blue and toluidine blue photobactericidal efficacy against Gram-positive and Gram-negative microorganisms. Lasers Surg Med 2001;29(2):165-173.
  67. Studies on curcumin and curcuminoids XXIX. photoinduced cytotoxicity of curcumin in selected preparations. Photochem Photobiol Sci 2005;4(7):523-530.
  68. Curcumin: the story so far. Eur J Cancer 2005;41:1955-1968.
  69. Dental caries. An infectious and transmissible disease. Pediatr Clin North Am 2000;47(5): 1001-1019.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.