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VOLUME 17 , ISSUE 3 ( March, 2016 ) > List of Articles


In vitro comparison of impact of different bleaching Agents on the microhardness of Enamel

Sudarshan C Pujari, Subhra Dey, Vinisha Pandey, Neha Awasthi

Citation Information : Pujari SC, Dey S, Pandey V, Awasthi N. In vitro comparison of impact of different bleaching Agents on the microhardness of Enamel. J Contemp Dent Pract 2016; 17 (3):258-262.

DOI: 10.5005/jp-journals-10024-1837

Published Online: 01-08-2016

Copyright Statement:  Copyright © 2016; The Author(s).



Various agents are used these days for increasing the esthetics. One such procedure is bleaching that offers various advantages, as it is minimal invasive and cheap option to color the teeth and remove stain. The altered enamel after the bleaching process shows surface demineralization and porosities. The present study aimed to evaluate the effect of different bleaching agents on the microhardness of enamel.

Materials and methods

A total of 100 freshly human extracted maxillary premolar teeth were selected for the study. Teeth with sound tooth structure were included for the study. All the specimens were randomly divided into four groups with 25 specimens in each group depending upon the type of bleaching agent used: Group A, artificial saliva (Control group); Group B, 35% hydrogen peroxide (HP); Group C, 25% HP; Group D, 10% carbamide peroxide (CP). Knoop Hardness Number (KHN) was calculated at 24, 48-hour, and 7-week interval.


Results showed no statistical significant differences between the microhardness of enamel of different groups (p < 0.005). A slight fall in the value of KHN was seen in all the groups, except for the control group, although the results were statistically nonsignificant (p > 0.005).


Although nonsignificantly, all the bleaching solutions produced some amount of alterations in the microstructure of enamel. More studies with higher study groups and more advanced estimation technologies are required to minimize microstructure alterations and promote for better outcome of bleaching procedures.

How to cite this article

Dey S, Pandey V, Kumar A, Awasthi N, Sahu A, Pujari SC. In vitro comparison of impact of different bleaching Agents on the microhardness of Enamel. J Contemp Dent Pract 2016;17(3):258-262.

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