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VOLUME 17 , ISSUE 8 ( August, 2016 ) > List of Articles

RESEARCH ARTICLE

Antibacterial Activity of Leaf Extract of Annona muricata and Simarouba glauca on Enterococcus faecalis

Jain Mathew, Reshmi George, Robin Theruvil, Tobin C Padavil, Lincy Tomy, Anil Kurian

Citation Information : Mathew J, George R, Theruvil R, Padavil TC, Tomy L, Kurian A. Antibacterial Activity of Leaf Extract of Annona muricata and Simarouba glauca on Enterococcus faecalis. J Contemp Dent Pract 2016; 17 (8):650-653.

DOI: 10.5005/jp-journals-10024-1906

Published Online: 01-08-2016

Copyright Statement:  Copyright © 2016; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim

To determine the antimicrobial effect of water extracts of leaves of Annona muricata and Simarouba glauca on Enterococcus faecalis using agar diffusion method.

Materials and methods

Dried leaves of A. muricata and S. glauca were powdered and extracted in a soxhlet apparatus. Enterococcus faecalis was grown overnight in Trypticase soy agar plates. About 10 μL of each extract was placed on agar plates and incubated overnight. The zone of inhibition was measured after 24 hours. About 1% sodium hypochlorite and distilled water were used as positive and negative controls.

Results

The leaf extract of A. muricata showed similar effectiveness as that of sodium hypochlorite, whereas the leaf extract of S. glauca showed only a slight reduction in growth of E. faecalis.

Conclusion

Leaf extract of A. muricata can be developed as an alternative to sodium hypochlorite for root canal irrigants.

Clinical significance

Success of endodontic treatment depends on complete disinfection of the root canals. Root canal irrigants have a major role in complete disinfection of the root canals. Chemical root canal irrigants are more or less toxic to the oral environment. In this study, naturally derived leaf extracts of A. muricata and S. glauca are compared with sodium hypochlorite for its effectiveness against E. faecalis – the most common pathogen found in the root canals.

How to cite this article

Mathew J, George R, Theruvil R, Padavil TC, Tomy L, Kurian A. Antibacterial Activity of Leaf Extract of Annona muricata and Simarouba glauca on Enterococcus faecalis. J Contemp Dent Pract 2016;17(8):650-653.


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  1. Microorganisms from canals of root-filled teeth with periapical lesions. Int Endod J 2003 Jan;36(1):1-11.
  2. The antibacterial effect of camphorated paramonochlorophenol, camphorated phenol and calcium hydroxide in the treatment of infected root canals. Endod Dent Traumatol 1985 Oct;1(5):170-175.
  3. Variations in the susceptibilities of components of the endodontic microflora to biomechanical procedures. Int Endod J 1996 Jul;29(4):235-241.
  4. Microbiological status of root-filled teeth with apical periodontitis. Int Endod J 1998 Jan;31(1):1-7.
  5. Microbiological findings and clinical treatment procedures in endodontic cases selected for microbiological investigation. Int Endod J 1997 Mar;30(2):91-95.
  6. Root canal irrigants. J Conserv Dent 2010 Oct;13(4):256-264.
  7. Phytochemical screening and assessment of antibacterial activity for the bioactive compounds in Annona muricata. Int J Curr Microbiol App Sci 2013;2(1):1-8.
  8. Preliminary screening of antibacterial and antitumour activities of Papua New Guinean native medicinal plants. Int J Pharmacog 1993;31(1):3-6.
  9. Anticancer, antioxidant and antimicrobial activity of annonaceae family. World J Pharm Pharm Sci 2014 Jan;3(3):1595-1604.
  10. In vitro antibacterial, antioxidant, haemolytic, thrombolytic activities and phytochemical analysis of Simarouba glauca leaves extracts. Int J Pharm Sci Res 2014 Feb;5(2):432-437.
  11. A critical review on medicinally important oil yielding plant Laxmitaru (Simarouba glauca DC). J Pharm Sci Res 2011;3(4):1195-1213.
  12. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 2006 Feb;32(2):93-98.
  13. Association of Enterococcus faecalis with different forms of periradicular diseases. J Endod 2004 May;30(5):315-320.
  14. Adverse reaction of sodium hypochlorite during endodontic treatment of primary teeth. Int J Clin Pediatr Dent 2015 May;8(2):153-156.
  15. Evaluation of antimicrobial efficacy of herbal alternatives (Triphala and green tea polyphenols), MTAD and 5% sodium hypochlorite against Enterococcus faecalis biofilm formed on tooth substrate: an in vitro study. J Endod 2010 Jan;36(1):83-86.
  16. Review: the use of sodium hypochlorite in endodontics – potential complications and their management. Br Dent J 2007 May;202(9):555-559.
  17. Antioxidant, DNA protective efficacy and HPLC analysis of Annona muricata (soursop) extracts. J Food Sci Technol 2015 Apr;52(4):2328-2335.
  18. Annona muricata: a comprehensive review on its traditional medicinal uses, phytochemicals, pharmacological activities, mechanisms of action and toxicity. Arabian J Chem 2016 Jan.
  19. Phytochemical and pharmacological properties of Annona muricata: a review. Int J Pharm Pharm Sci 2012;4(2):3-6.
  20. Evaluation of antioxidant and free radical scavenging activity of Annona muricata. Eur J Exp Biol 2015;5(3):39-45.
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