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VOLUME 17 , ISSUE 8 ( August, 2016 ) > List of Articles


Assessment of Correlation between Clinical Parameters and Pulp Canal Pathogens in Endodontic Pathologies: A Microbiological Study

Vinisha Pandey, Isha Choudhary, Vipin Kumar, Prashant Tripathi, Abhinav Misra, Hiroj Bagde

Citation Information : Pandey V, Choudhary I, Kumar V, Tripathi P, Misra A, Bagde H. Assessment of Correlation between Clinical Parameters and Pulp Canal Pathogens in Endodontic Pathologies: A Microbiological Study. J Contemp Dent Pract 2016; 17 (8):654-658.

DOI: 10.5005/jp-journals-10024-1907

Published Online: 01-12-2016

Copyright Statement:  Copyright © 2016; The Author(s).



Success and failure of root canal therapy is largely dependent on the clinician and the patient. Once the tooth is restored into its functional and esthetic place, the root canal therapy is considered to be complete. It is a well-known fact that root canal space acts as a rich reservoir for microorganisms. Past studies show that root canal harbors an array of different microorganisms. Streptococcus mitis and Enterococcus faecalis have been found to be the most prevalent microorganisms isolated from the infected pulp canal space. Hence, we evaluated the association of endodontic signs and symptoms with root canal pathogens.

Materials and methods

A total of 120 patients were selected for the study and divided broadly into two groups: Group I containing patients with primary infection and group II containing patients in which retreatment was required. Both groups contained 60 patients each. Clinical parameters recorded for the study are pain, tenderness on percussion, swelling, periapical radiolucency on radiographic analysis, caries, sinus formation, and tooth mobility. After assessment, opening, and initial filing, sterile paper points were inserted into the root canal space for collection of microbiological sample, which was further send to laboratory for cultural analysis. All the results were analyzed by Statistical Package for the Social Sciences (SPSS) software. Student's t-test and Fisher's exact test were used to evaluate the level of significance.


While comparing the mean age of the patients in the two groups, no significant association was seen. Statistically significant results were obtained while comparing the association between pain and S. mitis. Positive correlation was seen on comparing the association between tenderness on percussion and E. faecalis. Also, a positive association was seen between periapical radiolucency and S. mitis.


Strong positive correlation exists between S. mitis and E. faecalis and endodontic signs and symptoms.

Clinical significance

Sound knowledge regarding the association of microbes of root canal and specific endodontic signs and symptoms could help therapeutic management of root canal infections effectively.

How to cite this article

Pandey V, Choudhary I, Kumar V, Tripathi P, Misra A, Bagde H. Assessment of Correlation between Clinical Parameters and Pulp Canal Pathogens in Endodontic Pathologies: A Microbiological Study. J Contemp Dent Pract 2016;17(8):654-658.

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  1. Prevalence and risk factors of apical periodontitis in endodontically treated teeth in a selected population of Brazilian adults. Braz Dent J 2008;19(1):34-39.
  2. A la recherche du maillon faible: initiation aux facteurs humans. São Paulo: VC Cultural; 2013. p. 154.
  3. Oral bacteria and apial periodontitis: an experimental study in monkeys [Thesis]. Sweden: University of Goteborg; 1982.
  4. Life as an endodontic pathogen. Etiological differences between untreated and root-filled root canals. Endod Topics 2003 Nov;6(1):3-28.
  5. Persistent, recurrent, and acquired infection of the root canal system post-treatment. Endod Topics 2003;6:29-56.
  6. Intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy resistant periapical lesions: a longterm light and electron microscopic follow-up study. J Endod 1990 Dec;16(12):580-588.
  7. Characterization of first oral vagococcus isolate from a rootfilled tooth with periradicular lesions. Curr Microbiol 2008 Sep;57(3):235-238.
  8. Microbial flora of root canal-treated teeth associated with asymptomatic periapical radiolucent lesions. Oral Microbiol Immunol 2001 Dec;16(6):332-337.
  9. Diversity of endodontic microbiota revisited. J Dent Res 2009 Nov;88(11):969-981.
  10. Bacterial diversity in human subgingival plaque. J Bacteriol 2001 Jun;183(12):3370-3383.
  11. 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community. Nature 1990 May 3;345(6270):63-65.
  12. Endodontic infections: concepts, paradigms and perspectives. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002 Sep;94(3):281-293.
  13. Microbiological evaluation of infected root canals and their correlation with pain. RSBO 2012 Jan-Mar;9(1):31-37.
  14. Association of endodontic signs and symptoms with root canal pathogens: a clinical comparative study. Saudi Endod J 2016;6(2):82-86.
  15. Microbiological examination of infected dental root canals. Oral Microbiol Immunol 2004 Apr;19(2):71-76.
  16. Association of Enterococcus faecalis with different forms of periradicular diseases. J Endod 2004 May;30(5):315-320.
  17. Microbial flora of root canals of pulpally-infected teeth: Enterococcus faecalis a prevalent species. J Dent Res Dent Clin Dent Prospects 2009 Winter;3(1):24-27.
  18. Streptococci from root canals in teeth with apical periodontitis receiving endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005 Aug;100(2):232-241.
  19. Comprehensive analysis of secondary dental root canal infections: a combination of culture and culture-independent approaches reveals new insights. PLoS One 2012;7(11):e49576.
  20. Bacteriological identification of selected pathogens in infected primary and young permanent teeth associated with clinical symptoms. Open J Med Microbiol 2015 Jun;5(2):59-68.
  21. Endodontic microbiology. J Conser Dent 2010 Oct;13(4):232-239.
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