The Journal of Contemporary Dental Practice

Register      Login



Volume / Issue

Online First

Related articles

VOLUME 18 , ISSUE 1 ( January, 2017 ) > List of Articles


Effect of Tannic Acid on Bond Strength of Etch and Rinse and Self-etch Adhesive Systems in Dentin of Primary Teeth

Mojtaba Abdollahi, Masoumeh Ebrahimi, Alireza Sarraf Shirazi, Faraneh Abdolhoseinpour

Citation Information : Abdollahi M, Ebrahimi M, Shirazi AS, Abdolhoseinpour F. Effect of Tannic Acid on Bond Strength of Etch and Rinse and Self-etch Adhesive Systems in Dentin of Primary Teeth. J Contemp Dent Pract 2017; 18 (1):34-38.

DOI: 10.5005/jp-journals-10024-1984

Published Online: 01-05-2017

Copyright Statement:  Copyright © 2017; The Author(s).



One of the possible mechanisms for the gradual destruction of bond strength in dentin-resin interface, could be due to the demineralized unstable collagen matrix. Use of protease inhibitors, such as tannic acid (TA) could prevent destruction of collagen fibers. The aim of this study was to compare the TA effect on bond strength of etch and rinse and self-etch adhesive systems in the dentin of primary teeth.

Materials and methods

This in vitro study was done on 40 extracted primary molar teeth. The teeth were sectioned in the mesiodistal direction, and enamel of buccal and lingual surfaces was removed. Samples were randomly divided into four groups: Single bond (SB) + TA, SB, Clearfil SE Bond (CSB) + TA, and CSB. Then, Z250 and Clearfil AP-X composites were cured on the surfaces of SB and CSB groups respectively. After that, all samples were divided into aging and non-aging groups. For 3 months, samples were placed under 1,000 thermal cycles in aging group. Subsequently, the shear bond strengths of all groups were measured by the International testing machine, and failure mode was evaluated by an optical stereomicroscope. Data were analyzed with paired t-test and independent t-test.


Tannic acid induced a significant reduction in the immediate bond strength of adhesive SB. Meanwhile, TA had no significant effect on shear bond strength of the CSB system.


Based on our findings, use of TA is not recommended with SB and CSB adhesives on primary teeth.

Clinical significance

Tannic acid may not be considered in resin restorations of primary teeth.

How to cite this article

Ebrahimi M, Sarraf Shirazi A, Abdolhoseinpour F, Abdollahi M. Effect of Tannic Acid on Bond Strength of Etch and Rinse and Self-etch Adhesive Systems in Dentin of Primary Teeth. J Contemp Dent Pract 2017;18(1):34-38.

PDF Share
  1. Microstructure of mineralized tissues in human primary teeth. J Clin Pediatr Dent 2011 Spring;35(3):295-300.
  2. McDonald and Avery's dentistry for the child and adolescent. Elsevier Health Sciences; 2015.
  3. Four-year water degradation of total-etch adhesives bonded to dentin. J Dent Res. 2003;82(2):136-140.
  4. Characterisation of resin-dentine interfaces by compressive cyclic loading. Biomaterials 2005 May;26(14):2043-2052.
  5. Effects of light-curing time on the cytotoxicity of a restorative composite resin on odontoblast-like cells. J Appl Oral Sci 2010 Sep-Oct;18(5):461-466.
  6. Host-derived proteinases and degradation of dentine collagen in situ. Caries Res 2003 Jan-Feb;37(1):58-65.
  7. Collagen degradation by host-derived enzymes during aging. J Dent Res 2004 Mar;83(3):216-221.
  8. Changes in stiffness of demineralized dentin following application of collagen crosslinkers. J Biomed Mater Res B Appl Biomater 2008 Aug;86(2):330-334.
  9. Mechanical properties of tannic-acid-treated dentin matrix. J Dent Res 2009 Sep;88(9):807-811.
  10. Durability of solvent-free one-step self-etch adhesive under simulated intrapulpal pressure. J Clin Exp Dent 2015 Oct 1;7(4):e466-e470.
  11. A critical review of the durability of adhesion to tooth tissue: methods and results. J Dent Res 2005 Feb;84(2):118-132.
  12. Histomorphologic characterization and bond strength evaluation of caries-affected dentin/resin interfaces: effects of long-term water exposure. Dent Mater 2008 Jun;24(6):786-798.
  13. Effect of long-term water aging on microtensile bond strength of self-etch adhesives to dentin. Am J Dent 2010 Feb;23(1):29-33.
  14. Four-year water degradation of a total-etch and two self-etching adhesives bonded to dentin. J Dent 2008 Aug;36(8):611-617.
  15. Chlorhexidine increases the longevity of in vivo resin-dentin bonds. Eur J Oral Sci 2010 Aug;118(4):411-416.
  16. Microtensile bond strength of several adhesive systems to different dentin depths. Am J Dent 2003 Oct;16(5):292-298.
  17. Effect of collagenase and esterase on resin-dentin interface: a comparative study between a total-etch adhesive and a self-etch adhesive. Am J Dent 2009 Oct;22(5):295-298.
  18. A scanning electron microscopic study of tooth surface changes induced by tannic acid. J Prosthet Dent 1998 Feb;79(2):169-174.
  19. A pilot study of exposure of the smear layer to tannic acid solutions. J Prosthet Dent 1993 Sep;70(3):261-263.
  20. The effect of 25% tannic acid on prepared dentin: a scanning electron microscope-methylene blue dye study. J Prosthet Dent 1990 Jul;64(1):12-16.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.