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VOLUME 18 , ISSUE 10 ( October, 2017 ) > List of Articles


Morphology of the Dentin–resin Interface yielded by Two-step Etch-and-rinse Adhesives with Different Solvents

Joao Cardoso Ferreira, Patrícia T Pires, Álvaro F de Azevedo, Sofia Arantes-Oliveira, Mário J Silva, Paulo R de Melo

Citation Information : Ferreira JC, Pires PT, de Azevedo ÁF, Arantes-Oliveira S, Silva MJ, de Melo PR. Morphology of the Dentin–resin Interface yielded by Two-step Etch-and-rinse Adhesives with Different Solvents. J Contemp Dent Pract 2017; 18 (10):947-958.

DOI: 10.5005/jp-journals-10024-2155

Published Online: 01-12-2015

Copyright Statement:  Copyright © 2017; The Author(s).



The study aimed to analyze the morphology of the dentin–resin interface yielded by two-step etch-and-rinse adhesive systems with different solvents and compositions.

Materials and methods

A total of 32 dentine disks were prepared and randomly assigned to four groups of one-bottle etchand-rinse adhesive systems containing different solvents: group I, Adper Scotchbond-1XT™ (ethanol/water); group II, XP-Bond™ (tertiary butanol); group III, Prime and Bond NT® (acetone); and group IV, One Coat bond® (5% water). Adhesive systems were applied onto dentin disks, which were then thermal cycled, divided into two hemi-disks (n = 16), and prepared for field-emission scanning electron microscopy to examine the dentin–resin interdiffusion zone. Microphotographs were scanned and data were processed. Data were compared with analysis of variance multivariant test after Kolmogorov–Smirnov and Shapiro–Wilk tests using Statistic Package for the Social Sciences.


The adhesive layer thickness average found was group I: 45.9 ± 13.41 μm, group II: 20.6 ± 16.32 μm, group III: 17.7 ± 11.75 μm, and group IV: 50.7 ± 27.81 μm. Significant differences were found between groups I and IV and groups II and III (p<0.000).

Groups I (3.23 ± 0.53 μm) and II (3.13 ± 0.73 μm) yielded significantly thicker hybrid layers than groups III (2.53 ± 0.50 μm) and IV (1.84 ± 0.27 μm) (p<0.003). Group III presented a less homogeneous hybrid layer, with some gaps. Tag length average was greater in groups II (111.0 ± 36.92 μm) and IV (128.9 ± 78.38 μm) than in groups I (61.5 ± 18.10 μm) and III (68.6 ± 15.84 μm) (p<0.008).


Adhesives systems with different solvents led to significant differences in the dentin–resin interface morphology. Solvents role in adhesives bond strength should be considered together with the other adhesive system components.

Clinical significance

The adhesive containing tertiary butanol, in addition, seems to originate a good-quality hybrid layer and long, entangled tags and also appears to have greater ability to originate microtags, which may indicate higher bond strength.

How to cite this article

Ferreira JC, Pires PT, de Azevedo ÁF, Arantes-Oliveira S, Silva MJ, de Melo PR. Morphology of the Dentin–resin Interface yielded by Two-step Etch-and-rinse Adhesives with Different Solvents. J Contemp Dent Pract 2017;18(10):947-958.

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