The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 18 , ISSUE 10 ( October, 2017 ) > List of Articles

RESEARCH ARTICLE

Comparative Evaluation of Myeloperoxidase Enzymatic Activity in Gingival Crevicular Fluid of Subjects having Orthodontic Treatment by Different Aligning Arch Wires

Vinny Bhasin, Madhavi Singh, Manish Goutam, Shuchi Singh, Adamya S Nigam, Aditya Joshi

Citation Information : Bhasin V, Singh M, Goutam M, Singh S, Nigam AS, Joshi A. Comparative Evaluation of Myeloperoxidase Enzymatic Activity in Gingival Crevicular Fluid of Subjects having Orthodontic Treatment by Different Aligning Arch Wires. J Contemp Dent Pract 2017; 18 (10):977-980.

DOI: 10.5005/jp-journals-10024-2159

Published Online: 01-10-2017

Copyright Statement:  Copyright © 2017; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Introduction

There exist a number of factors that affect the outcome of orthodontic treatment. These factors can be assessed by various gingival markers. One such maker is myeloperoxidase (MPO). Hence, we planned the present study to assess and compare the MPO activity in the gingival crevicular fluid (GCF) of subjects undergoing orthodontic treatment by different aligning arch wires.

Materials and methods

The present study included assessment of patients who underwent orthodontic treatment for crowding of anterior teeth. Diagnostic cast models of all the subjects were made for recording the irregularity index. All the subjects were randomly divided into three study groups with 15 patients in each group based on the type of nickel–titanium (NiTi) arch wires used. A collection of GCF samples was done in all the patients at various time intervals and it was sent to the laboratory for assessment of MPO activity. Activity of the MPO enzyme was expressed in terms of number of units per 100 µL. All the results obtained were compiled and analyzed by Statistical Package for the Social Sciences (SPSS) software.

Results

We observed that nonsignificant results were obtained while comparing the mean age and mean gingival score in all the study groups. However, significant results were obtained on comparing the mean MPO enzymatic activity in all the study groups at different time intervals.

Conclusion

Both superelastic NiTi and heat-activated NiTi generate optimal forces, which are necessary for higher metabolic response of the periodontal ligament.

Clinical significance

In the intimal stages of orthodontic treatment, both superelastic NiTi and heat-activated NiTi wires are superior in leveling and aligning the crowded teeth.

How to cite this article

Bhasin V, Singh M, Goutam M, Singh S, Nigam AS, Joshi A. Comparative Evaluation of Myeloperoxidase Enzymatic Activity in Gingival Crevicular Fluid of Subjects having Orthodontic Treatment by Different Aligning Arch Wires. J Contemp Dent Pract 2017;18(10):977-980.


PDF Share
  1. The influence of orthodontic movement on periodontal tissues level. Semin Orthod 2007 Dec;13(4):234-245.
  2. Cellular, molecular, and tissue-level reactions to orthodontic force. Am J Orthod Dentofacial Orthop 2006 Apr;129(4):469.e1-469.e32.
  3. Medication effects on the rate of orthodontic tooth movement: a systematic literature review. Am J Orthod Dentofacial Orthop 2009 Jan;135(1):16-26.
  4. Diagnostic biomarkers for oral and periodontal diseases. Dent Clin North Am 2005 Jul;49(3):551-571, vi.
  5. Molecular markers of early orthodontic tooth movement. Angle Orthod 2009 Nov;79(6):1108-1113.
  6. Expression of mRNA for osteoprotegerin and receptor activator of nuclear factor kappa beta ligand (RANKL) during root resorption induced by the application of heavy orthodontic forces on rat molars. Am J Orthod Dentofacial Orthop 2005 Oct;128(4):497-503.
  7. Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation. Cell 1998 Apr;93(2):165-176.
  8. Cytokine expression pattern in compression and tension sides of the periodontal ligament during orthodontic tooth movement in humans. Eur J Oral Sci 2007 Oct;115(5):355-362.
  9. Bidirectional signaling between stromal and hemopoietic cells regulates interleukin-1 expression during human osteoclast formation. Bone 1999 Sep;25(3):269-278.
  10. A comparative evaluation of static frictional resistance using various methods of ligation at different time intervals: an in vitro study. Int J Dent 2015 Apr;2015:407361.
  11. The load/deflection characteristics of thermally activated orthodontic archwires. Eur J Orthod 2003 Aug;25(4):417-421.
  12. Assay of the human leukocyte enzymes myeloperoxidase and eosinophil peroxidase. J Immunol Methods 1990 Jan;126(1):125-133.
  13. Cyclical tensile force on periodontal ligament cells inhibits osteoclastogenesis through OPG induction. J Dent Res 2006 May;85(5):457-462.
  14. Substance P increases production of proinflammatory cytokines and formation of osteoclasts in dental pulp fibroblasts in patients with severe orthodontic root resorption. Am J Orthod Dentofacial Orthop 2008 May;133(5):690-698.
  15. Tartrate-resistant acid phosphatase (TRACP): a personal perspective. J Bone Miner Res 2003 Oct;18(10):1894-1896.
  16. Relationship between lactate dehydrogenase activity in saliva and oral health status. Arch Oral Biol 2007 Oct;52(10):911-915.
  17. An evaluation and comparison of myeloperoxidase enzymatic activity during initial orthodontic alignment: an in vivo study. J Orthod 2017 Jul;13:1-5.
  18. Myeloperoxidase enzymatic activity is increased in patients with different levels of dental crowding after initial orthodontic activation. Am J Orthod Dentofacial Orthop 2014 Jul;146(1):92-97.
  19. Efficiency, behavior, and clinical properties of superelastic NiTi versus multistranded stainless steel wires: a prospective clinical trial. Angle Orthod 2012 Sep;82(5):915-921.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.