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VOLUME 18 , ISSUE 11 ( November, 2017 ) > List of Articles


In situ Effect of Nanohydroxyapatite Paste in Enamel Teeth Bleaching

Cecy Martins Silva, Yasmin SB de Lima Gomes, Larissa D Alexandrino, Cristiane de M Alencar, Eliane B Alves, Kelson CF Faial

Citation Information : Silva CM, de Lima Gomes YS, Alexandrino LD, de M Alencar C, Alves EB, Faial KC. In situ Effect of Nanohydroxyapatite Paste in Enamel Teeth Bleaching. J Contemp Dent Pract 2017; 18 (11):996-1003.

DOI: 10.5005/jp-journals-10024-2164

Published Online: 01-03-2017

Copyright Statement:  Copyright © 2017; The Author(s).



Evaluate in situ the effect of nanohydroxyapatite paste (nano-HAP) before bleaching with hydrogen peroxide 35% (HP35%) by ion chromatography (IC) Knoop hardness number (KHN) and tristimulus colorimetry (TC).

Materials and methods

A total of 60 fragments were obtained from third molars included (3 mm × 3 mm × 3 mm) and the specimens were divided into three groups (n = 20): Gas chromatography (CG) (negative control group) = no bleaching; HP35% (positive control group) = HP35% whitening (whiteness HP35%); nano-HAP = application for 10 minutes before bleaching treatment + HP35%. The specimens were fixed to the volunteers’ molars. The KHN and TC were measured before and after bleaching. For IC, the dentin layer was removed, leaving the enamel that was crushed, and autoclaved for chemical quantification (calcium, fluorine, and phosphorus). The results of KHN and TC were analyzed statistically by analysis of variance (ANOVA) followed by Tukey test (p < 0.05).


The HP35% group showed reduction of the Ca, F, and P ions. The initial and final KHN mean of the CG and nano-HAP did not differ statistically; however, the group of HP35% did differ statistically. The mean ∆E of the HP35% and nano-HAP groups did not differ statistically from each other. However, they differed from the CG.


The nano-HAP paste preserved the KHN, promoted the lower loss of Ca and P ions and an increase of F ions when compared with the CG, but did not influence the effectiveness of the bleaching treatment.

Clinical significance

Nano-HA is a biomaterial that has shown positive results in the prevention of deleterious effects on the enamel by the action of the office bleaching treatment.

How to cite this article

Gomes YSBL, Alexandrino LD, Alencar CDM, Alves EB, Faial KCF, Silva CM. In situ Effect of Nanohydroxyapatite Paste in Enamel Teeth Bleaching. J Contemp Dent Pract 2017;18(11):996-1003.

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