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VOLUME 18 , ISSUE 9 ( September, 2017 ) > List of Articles


Immunohistochemical Expression of Cathepsin D in Primary and Recurrent Squamous Cell Carcinoma

Krishnanand P Satelur, GS Kumar

Citation Information : Satelur KP, Kumar G. Immunohistochemical Expression of Cathepsin D in Primary and Recurrent Squamous Cell Carcinoma. J Contemp Dent Pract 2017; 18 (9):795-801.

DOI: 10.5005/jp-journals-10024-2129

Published Online: 01-12-2017

Copyright Statement:  Copyright © 2017; The Author(s).



The aim of this study is to analyze and compare the immunohistochemical expression of cathepsin B in primary oral squamous cell carcinoma (OSCC) and recurrent OSCC.

Materials and methods

A total of 50 cases were studied immunohistochemically for rabbit polyclonal antihuman cathepsin D expression. A total of 10 cases of breast carcinoma were taken as positive controls. Immunohistochemical staining was performed using labeled streptavidin–biotin technique.


All the 45 cases of OSCC, both primary and recurrent cases included, showed varying grades of cathepsin D immunoreactivity. Statistical significance at 5% level was observed in cathepsin D expression between the different grades of well, moderate, and poorly differentiated primary squamous cell carcinomas. In the comparison of cathepsin D staining intensity among primary squamous cell carcinomas with and without recurrence, a statistical significance between the groups was observed when the p-value was at 10%, but the same comparison was not significant when the p-value was at 5%.


Cathepsin D expression in primary squamous cell carcinomas with recurrences was very variable as compared with primary squamous cell carcinomas without recurrences. Comparison of cathepsin D expression in primary with their recurrent counterparts showed mostly similar intensity of expression in recurrent carcinomas, thus suggesting its limited usefulness in predicting recurrence.

Clinical significance

Although cathepsin D might have shown limited usefulness in predicting cancer recurrence, it, however, is a proven valuable tool to detect the aggressiveness of other tumors, and if corroborated with a larger sample may hold the key to early, more effective, and more specific treatment modalities for cases of oral cancer also.

How to cite this article

Satelur KP, Kumar GS. Immunohistochemical Expression of Cathepsin D in Primary and Recurrent Squamous Cell Carcinoma. J Contemp Dent Pract 2017;18(9): 795-801.

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  1. Function and importance of p63 in normal oral mucosa and squamous cell carcinoma of the head and neck. Adv Otorhinolaryngol 2005 Feb;62:49-57.
  2. Immunocytochemical analysis of AE1/AE3, CK 14, Ki-67 and p53 expression in benign, premalignant and malignant oral tissue to establish putative markers for progression of oral carcinoma. Br J Biomed Sci 2004;61(3):117-124.
  3. ; Wilson, DF.; Daftary, DK.; Johnson, NW. Oral diseases in the tropics. Delhi, Bombay, Calcutta, Madras: Oxford University Press; 1993. p. 429.
  4. Anderson's pathology. 9th ed., Vol. 2. New Delhi: Jaypee Brothers; 1991. p. 1112.
  5. Head and neck cancer: a multidisciplinary approach. Surg Oncol 1999 Nov;8(3):167-176.
  6. Tissue cathepsins as tumor markers. Clin Chim Acta 1995 Jun;237(1-2):67-78.
  7. Cathepsin expression in oral squamous cell carcinoma: relationship with clinicopathologic factors. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002 Apr;93(4):446-454.
  8. Role of proteases in tumor invasion and metastasis. Indian J Exp Biol 1997 Jun;35(6):553-564.
  9. Variable expression of cathepsin B and D correlates with highly invasive and metastatic phenotype of oral cancer. Hum Pathol 2000 Aug;31(8):931-937.
  10. Differentiation and cathepsin D expression in human oral tumors. Laryngoscope 1998 Aug;108(8 Pt 1):1234-1237.
  11. Expression of cathepsin D in head and neck cancer. Eur J Cancer 1992;28A(8-9):1413-1415.
  12. Cathepsin B and D expression in squamous cell carcinoma. Br J Dermatol 1996 Dec;135(6):905-910.
  13. Immunohistochemical localization of cathepsins D and E in human gastric cancer: a possible correlation with local invasive and metastatic activities of carcinoma cells. Hum Pathol 1996 Feb;27(2):184-190.
  14. Elevated activity and increased mannose-6-phosphate in the carbohydrate moiety of cathepsin D from human hepatoma. Cancer Res 1988 Jan;48(2):362-367.
  15. Cathepsin D in normal and neoplastic thyroid tissues. Surgery 1995 Dec;118(6):1036-1040.
  16. Activity, expression, and transcription rate of the cathepsins B, D, H, and L in cutaneous malignant melanoma. Cancer 2001 Mar;91(5):972-982.
  17. Cathepsins in basal cell carcinomas: activity, immunoreactivity and mRNA staining of cathepsins B, D, H and L. Arch Dermatol Res 2004 Mar;295(10):411-421.
  18. Immunolocalization of cathepsin D in normal and neoplastic human tissues. J Clin Pathol 1986 Dec;39(12):1323-1330.
  19. Cathepsin D and prognosis in breast cancer. N Engl J Med 1990 Feb;322(5):297-302.
  20. Prognostic factors in early breast carcinoma. Cancer 1994 Jan;74(1 Suppl):381-400.
  21. Unraveling the role of proteases in cancer. Clin Chim Acta 2000 Feb;291(2):113-135.
  22. Cancer invasion and tissue remodeling-cooperation of protease systems and cell types. APMIS 1999 Mar;107(1-6):120-127.
  23. A novel aspartic protease gene, ALP56, is up-regulated in human breast cancer independently from the cathepsin D gene. Breast Cancer Res Treat 2003 Mar;78(1):37-44.
  24. Proteases cathepsins – a view. Biochem Educ 1990 Apr;18(2):67-72.
  25. The role of cell adhesion molecules and proteases in tumor invasion and metastasis. World J Urol 1996 Jun;14(3):151-156.
  26. Cathepsin B and cathepsin D expression in the progression of colorectal adenoma to carcinoma. Cancer Lett 2004 Mar;205(1):97-106.
  27. Detection of oral squamous cell carcinoma metastasis with cathepsin D: an immunohistochemical approach. Dent Res J (Isfahan) 2014 Mar-Apr;11(2):204-211.
  28. Clinicopathological significance of cathepsin D expression in gastric adenocarcinoma. Oncology 2001 Jul;61(1):71-78.
  29. Correlation between protein nm 23 and cathepsin D with clinical and pathological features of oral squamous cell carcinoma. Libr Oncol 2016 Dec;44(2-3):7-14.
  30. Immuno-histochemical evaluation of cathepsin D in malignant salivary gland carcinomas. Gulf J Oncolog 2012 Jan;11:38-44.
  31. The expression of E-cadherin and cathepsin-D in normal oral mucosa, oral epithelial dysplasia and oral squamous cell carcinoma: a comparative analysis between immunohistochemistry and routine histopathology. J Oral Maxillofac Pathol 2011 Sep-Dec;15(3):288-294.
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