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VOLUME 19 , ISSUE 12 ( December, 2018 ) > List of Articles

ORIGINAL ARTICLE

Evaluation of Radiation-induced Cytological Changes in Lesional Oral Cancer Cells and Adjacent Normal Mucosal Cells

Vinit Patil, Rajendra Baad, Nupura Vibhute, Uzma Belgaumi, Vidya Kadashetti, Anand Gudur

Keywords : Cytology, Oral cancer, Radiosensitivity

Citation Information : Patil V, Baad R, Vibhute N, Belgaumi U, Kadashetti V, Gudur A. Evaluation of Radiation-induced Cytological Changes in Lesional Oral Cancer Cells and Adjacent Normal Mucosal Cells. J Contemp Dent Pract 2018; 19 (12):1475-1480.

DOI: 10.5005/jp-journals-10024-2452

License: CC BY-NC 4.0

Published Online: 01-12-2018

Copyright Statement:  Copyright © 2018; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim: To assess various cytological changes for predicting radiosensitivity of oral squamous cell carcinoma by exfoliative cytology. Materials and methods: Histologically proven 30 cases of oral squamous cell carcinoma who underwent fractionated radiotherapy in a dose of 45-60 Gy in 5 fractions/week were enrolled in the study. The exfoliative cytology smear was evaluated on lesional and adjacent oral mucosa before radiotherapy, during radiotherapy (8 and 11th fraction) and post radiotherapy (4, 6 and 8 weeks). Various parameters like multinucleation, cellular enlargement, nuclear enlargement, cytoplasmic vacuolation, cytoplasmic granulation, leukocytic infiltration were evaluated. Results: Statistical significant values were seen in the intergroup comparison of all the parameters when compared adjacent mucosa and normal mucosa for leukocytic infiltration in pretreatment smear. Conclusion: The study showed that radiation-induced cytological changes in oral squamous cell carcinoma have a significant dose-related increase. This dose-response relationship and the high intratumoral variations suggest that serial assay of these changes has potential use for radiosensitivity prediction. Clinical significance: Radiosensitivity prediction can be evaluated by means of cytological smears in one stop crisis centre (OSCC) individuals subjected to fractionated radiotherapy by evaluating the cytological parameters.


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