The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 19 , ISSUE 3 ( March, 2018 ) > List of Articles

ORIGINAL RESEARCH

Assessment of Lipid Peroxidation Levels and Total Antioxidant Status in Chronic and Aggressive Periodontitis Patients: An in vivo Study

Ashish Verma, Vivek Tripathi, Sahib T Singh, Chetan D Singh, Jaspreet S Gill

Citation Information : Verma A, Tripathi V, Singh ST, Singh CD, Gill JS. Assessment of Lipid Peroxidation Levels and Total Antioxidant Status in Chronic and Aggressive Periodontitis Patients: An in vivo Study. J Contemp Dent Pract 2018; 19 (3):287-291.

DOI: 10.5005/jp-journals-10024-2254

License: CC BY 3.0

Published Online: 00-03-2018

Copyright Statement:  Copyright © 2018; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Introduction

Periodontitis is a common problem affecting a significant population of the world. For the assessment of oxidative stress of an individual, total oxidation status (TOS) and total antioxidant capacity (TAOC) are the significant biomarkers. Hence, we planned the present study to assess malondialdehyde (MDA), TOS, TAOC levels, and oxidative stress index (OSI) in generalized aggressive periodontitis (GP) and chronic periodontitis (CP) patients.

Materials and methods

The present study included assessment of 40 CP patients, 40 GP patients, and 40 healthy controls. Clinical assessment of all the subjects was done by measuring the probing depth (PD), clinical attachment (CL), gingival index (GI), gingival bleeding index (GBI), and plaque index (PI). Salivary and serum samples were taken and assessed by standard procedures as described previously in the literature. All the values were assessed and compared.

Results

Significant results were obtained while comparing all the periodontal parameters in between various study groups. Mean serum MDA levels in the CP, GP, and control group were found to be 0.68, 0.65, and 0.61 µM respectively. Statistically nonsignificant results were obtained while comparing the serum MDA levels in between the three study groups. Significant results were obtained while comparing the mean serum and salivary TOS values, TAOC values, and OSI in between various study groups.

Conclusion

In periodontitis patients, oxidative stress was significantly higher in comparison with healthy subjects.

Clinical significance

Oxidative parameters do play a significant role in the pathologic profile of periodontitis.

How to cite this article

Tripathi V, Singh ST, Sharma V, Verma A, Singh CD, Gill JS. Assessment of Lipid Peroxidation Levels and Total Antioxidant Status in Chronic and Aggressive Periodontitis Patients: An in vivo Study. J Contemp Dent Pract 2018;19(3):287-291.


  1. 8-hydroxy-deoxyguanosine levels in gingival crevicular fluid and saliva in patients with chronic periodontitis after initial periodontal treatment. J Periodontol 2013 Jun;84(6):821-828.
  2. The lipid peroxidation product 4-hydroxy-2-nonenal: advances in chemistry and analysis. Redox Biol 2013 Jan;1(1):145-152.
  3. The role of reactive oxygen and antioxidant species in periodontal tissue destruction. Periodontol 2000 2007 Feb;43(1):160-232.
  4. Matrix metalloproteinase-13 is highly expressed in destructive periodontal disease activity. J Periodontol 2006 Nov;77(11):1863-1870.
  5. Oral fluid-based biomarkers of alveolar bone loss in periodontitis. Ann N Y Acad Sci 2007 Mar;1098:230-251.
  6. Microfluidic immunoassays as rapid saliva-based clinical diagnostics. Proc Natl Acad Sci U S A 2007 Mar;104(13):5268-5273.
  7. The concepts of stress and stress system disorders. Overview of physical and behavioral homeostasis. JAMA 1992 Mar;267(9):1244-1252.
  8. Protein adducts generated from products of lipid oxidation: focus on HNE and one. Drug Metab Rev 2006 Feb;38(4):651-675.
  9. Development of a classification system for periodontal diseases and conditions. Ann Periodontol 1999 Dec;4(1):1-6.
  10. Case definitions for use in population-based surveillance of periodontitis. J Periodontol 2007 Jul;78(7 Suppl):1387-1399.
  11. Periodontal disease in pregnancy. I. Prevalence and severity. Acta Odontol Scand 1963 Dec;21:533-551.
  12. Gingival sulcus bleeding–a leading symptom in initial gingivitis. Helv Odontol Acta 1971 Oct;15(2):107-113.
  13. Periodontal disease in pregnancy. II. correlation between oral hygiene and periodontal condition. Acta Odontol Scand 1964 Feb;22:121-135.
  14. Lipid peroxidation levels and total oxidant status in serum, saliva and gingival crevicular fluid in patients with chronic periodontitis. J Clin Periodontol 2007 Jul;34(7):558-565.
  15. Periodontal disease is associated with lower antioxidant capacity in whole saliva and evidence of increased protein oxidation. Clin Sci (Lond) 2003 Aug;105(2):167-172.
  16. Measurement of malondialdehyde in plasma by high performance liquid chromatography with fluorimetric detection. Ann Clin Biochem 1991 Sep;28(Pt 5):504-508.
  17. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005 Dec;38(12):1103-1111.
  18. Lipid peroxidation levels and total oxidant/antioxidant status in serum and saliva from patients with chronic and aggressive periodontitis. Oxidative stress index: a new biomarker for periodontal disease? J Periodontol 2014 Oct;85(10):1432-1441.
  19. Total oxidant status and bone resorption biomarkers in serum and gingival crevicular fluid of patients with periodontitis. J Periodontol 2014 Feb;85(2):317-326.
  20. Lipid peroxidation levels, total oxidant status and superoxide dismutase in serum, saliva and gingival crevicular fluid in chronic periodontitis patients before and after periodontal therapy. Aust Dent J 2010 Mar;55(1):70-78.
  21. Oxidative stress, systemic inflammation, and severe periodontitis. J Dent Res 2010 Nov;89(11):1241-1246.
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.