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VOLUME 19 , ISSUE 4 ( 2018 ) > List of Articles


Antibacterial Effect and Tensile Bond Strength of Self-etching Adhesive Resins with and without Methacryloyloxydodecylpyridinium Bromide: An in vitro Study

Madhuram Krishnamurthy, V Naveen Kumar, Ashok Leburu, Kasiswamy E Selvendran, Nehrudhas Praveen

Keywords : Antibacterial property, Methacryloyloxydodecylpyridinium bromide, Self-etching primers, Tensile bond strength

Citation Information : Krishnamurthy M, Kumar VN, Leburu A, Selvendran KE, Praveen N. Antibacterial Effect and Tensile Bond Strength of Self-etching Adhesive Resins with and without Methacryloyloxydodecylpyridinium Bromide: An in vitro Study. J Contemp Dent Pract 2018; 19 (4):409-414.

DOI: 10.5005/jp-journals-10024-2275

License: CC BY-NC 3.0

Published Online: 01-04-2018

Copyright Statement:  Copyright © 2018; The Author(s).


Aim: The aim of the present study was to compare the antibacterial activity of a self-etching primer containing antibacterial monomer methacryloyloxydodecylpyridinium bromide (MDPB) (Clearfil protect bond) with a conventional self-etching primer without MDPB (Clearfil SE bond) against Streptococcus mutans and the effect of incorporation of MDPB on the tensile bond strength of the experimental self-etching primer (Clearfil protect bond). Materials and methods: The antibacterial activity of the selfetching primers was assessed using agar disk diffusion method and the diameters of the zones of inhibition were measured and ranked. For tensile bond strength testing, 20 noncarious human molars were selected and randomly divided into two groups comprising 10 teeth in each group. Group I specimens were treated with Clearfil SE bond (without MDPB). Group II specimens were treated with Clearfil protect bond (with MDPB). Composite material was placed incrementally and cured for 40 seconds in all the specimens. Tensile bond strength was estimated using the Instron Universal testing machine at a crosshead speed of 1 mm/min. Results: The addition of MDPB into a self-etching primer exerts potential antibacterial effect against S. mutans. The tensile bond strength of MDPB containing self-etching primer was slightly lower than that of the conventional self-etching Clearfil protect bond primer, but the difference was not statistically significant. Conclusion: Thus, a self-etching primer containing MDPB will be a boon to adhesive dentistry as it has bactericidal property with adequate tensile bond strength. Clinical significance: The concept of prevention of extension in adhesive dentistry would result in micro/nanoleakage due to the presence of residual bacteria in the cavity. Self-etching primers with MDPB would improve the longevity of such restorations by providing adequate antibacterial activity without compromising the bond strength.

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  1. Buonocore MG. A simple method of increasing the adhesion of acrylic filling materials to enamel surfaces. J Dent Res 1955 Dec;34(6):849-853.
  2. Karanika-Kouma A, Dionysopoulos P, Koliniotou-Koubia E, Kolokotronis A. Antibacterial properties of dentin bonding systems, polyacid-modified composite resins and composite resins. J Oral Rehabil 2001 Feb;28(2):157-160.
  3. Imazato S, Ebi N, Tarumi H, Russell RR, Kaneko T, Ebisu S. Bactericidal activity and cytotoxicity of antibacterial monomer MDPB. Biomaterials 1999 May;20(9):899-903.
  4. Slutzky H, Matalon S, Weiss EI. Antibacterial surface properties of polymerized single-bottle bonding agents: part II. Quintessence Int 2004 Apr;35(4):275-279.
  5. Imazato S, Torii Y, Takatsuka T, Inoue K, Ebi N, Ebisu S. Bactericidal effect of dentin primer containing antibacterial monomer methacryloyloxydodecylpyridinium bromide (MDPB) against bacteria in human carious dentin. J Oral Rehabil 2001 Apr;28(4):314-319.
  6. Burrow MF, Tagami J, Negishi T, Nikaido T, Hosoda H. Early tensile bond strengths of several enamel and dentin bonding systems. J Dent Res 1994 Feb;79(2):522-528.
  7. Kudou Y, Obara K, Kawashima T, Kubota M, Abe S, Endo T, Komatsu M, Okuda R. Addition of antibacterial agents to MMA-TBB dentin bonding systems—influence on tensile bond strength and antibacterial effect. Dent Mater J 2000 Mar;19(1):65-74.
  8. Imazato S, Kaneko T, Takahashi Y, Noiri Y, Ebisu S. In vivo antibacterial effects of dentin primer incorporating MDPB. Oper Dent 2004 Jul-Aug;29(4):369-375.
  9. Yudovin-Farber I, Beyth N, Weiss EI, Domb AJ. Antibacterial effect of composite resins containing quaternary ammonium polyethyleneimine nano particles. J Nanopart Res 2010 Feb;12(2):591-603.
  10. Imazato S, Ehara A, Torii M, Ebisu S. Antibacterial activity of dentine primer containing MDPB after curing. J Dent 1998 Mar;26(3):267-271.
  11. Imazato S, Kinomoto Y, Tarumi H, Ebisu S, Tay FR. Antibacterial activity and bonding characteristics of an adhesive resin containing antibacterial monomer MDPB. Dent Mater 2003 Jun;19(4):313-319.
  12. Imazato S, Kinomoto Y, Tarumi H, Torii M, Russell RR, McCabe JF. Incorporation of antibacterial monomer MDPB into dentin primer. J Dent Res 1997 Mar;76(3):768-772.
  13. Imazato S, Kuramoto A, Kaneko T, Ebisu S, Russell RR. Comparison of antibacterial activity of simplified adhesive systems. Am J Dent 2002 Dec;15(6):356-360.
  14. Duyzol M, Karamese M, Akgul N, Gul P, Celik N, Duzyol E, Altoparlak U. Comparison of the antibacterial efficacy of several dentin bonding agents: two different in vitro studies. Int J Dent Sci Res 2016 Jan;4(1):1-4.
  15. Sampath PB, Hegde MN, Hegde P. Assessment of antibacterial properties of newer dentin bonding agents: an in vitro study. Contemp Clin Dent 2011 Jul;2(3):165-169.
  16. Yoshikawa K, Clark DT, Brailsford SR, Beighton D, Watson TF, Imazato S, Momoi Y. The effect of antibacterial monomer MDPB on the growth of organisms associated with root caries. Dent Mater J 2007 May;26(3):388-392.
  17. Imazato S, Kuramoto A, Takahashi Y, Ebisu S, Peters MC. In vitro antibacterial effects of the dentin primer of Clearfil protect bond. Dent Mater 2006 Jun;22(6):527-532.
  18. Korkmaz Y, Ozalp M, Attar N. Comparison of the antibacterial activity of different self-etching primers and adhesives. J Contemp Dent Pract 2008 Nov;9(7):57-64.
  19. Gupta S, Vellanki VK, Shetty VK, Kushwah S, Goel G, Chandra SM. In vitro evaluation of shear bond strength of nano composites to dentin. J Clin Diagn Res 2015 Jan;9(1):ZC09-ZC11.
  20. Beyth N, Yudovin-Farber I, Ervin AJ, Weiss I. Long-term antibacterial surface properties of composite resin incorporating polyethyleneimine nano particles. Quintessence Int 2010 Nov-Dec;41(10):827-835.
  21. Rathke A, Staude R, Muche R, Haller B. Anti-bacterial activity of a triclosan containing resin composite matrix against three common oral bacteria. J Mater Sci Mater Med 2010 Nov;21(11):2971-2977.
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