The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 20 , ISSUE 2 ( February, 2019 ) > List of Articles

ORIGINAL RESEARCH

Evaluation of Proliferative Marker Ki-67 in Adenoid Cystic Carcinoma: A Retrospective Study

Pavan G Kulkarni, Smita Bussari, Manish Sarode, Prabhakar A Jeergal, Anjum Deshmukh, Nasim AK Namazi

Keywords : Adenoid cystic carcinoma, Ki-67, Immuno-histochemistry

Citation Information : Kulkarni PG, Bussari S, Sarode M, Jeergal PA, Deshmukh A, Namazi NA. Evaluation of Proliferative Marker Ki-67 in Adenoid Cystic Carcinoma: A Retrospective Study. J Contemp Dent Pract 2019; 20 (2):211-215.

DOI: 10.5005/jp-journals-10024-2499

License: CC BY-NC 4.0

Published Online: 01-02-2019

Copyright Statement:  Copyright © 2019; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim: Adenoid cystic carcinoma (ACC) is a malignant tumor of salivary gland origin. Although the histologic appearance of ACC is low grade, management of this malignancy is a distinct therapeutic challenge because of its tendency for perineural involvement and potential for distant metastasis. Ki-67 antigen is expressed during the G1, S, G2 and M phases in the cell cycle but is absent in the quiescent G0 phase in tissue sections. Aim of the study was to review hematoxylin and eosin stained slides in order to confirm the previous histopathological diagnosis as per the criteria given by World Health Organisation (WHO) and to evaluate the expression of cell proliferation marker, Ki-67 antigen in Adenoid cystic carcinoma and correlate the expression of Ki-67 antigen histopathologically with different grades in Adenoid cystic carcinoma. Materials and methods: Tissue samples of 32 cases (12 males and 20 females) were selected from minor salivary glands with age range from 21 to 70 years. Two paraffin-embedded sections of these total 32 cases each of 4 μm thick were cut on a rotary microtome. One section was stained using hematoxylin and Eosin (H&E) and the other was used for Immunohistochemical staining with Ki-67 antigen. Results: Among these 32 cases of Adenoid cystic carcinoma, Histologically 14 (43.75%) tumors were classified in grade I, 8 (25%) were in grade II, and 10 (31.25%) were in grade III. The average percentage of Ki-67 expression was 27.12% in grade I, 34.43% in grade II and 38.45% in grade III. Conclusion: Ki-67 immunoreactivity increased with increase in histopathological grades of ACC. Clinical significance: Since Ki-67 is a useful marker for assessing the proliferative potential of tumors, the prognosis of patients can definitely be predicted.


PDF Share
  1. Ellis Gl, Auclair PL, Gnepp DR. Surgical pathology of salivary glands. Philapdelphia: WB Saunders Company; 1991:108-164.
  2. Rapidis AD, Givalos N, Gakiopoulou H, Faratzis G, Stavrianos SD, Vilos GA et al. Adenoid cystic carcinoma of head and neck. Clinicopathological analysis of 23 patients and review of the literature. Oral Oncolog. 2005:41:328-335.
  3. Szanto PA, Luna MA, Tortoledo ME, White RA. Histologic grading of adenoid cystic carcinoma of the salivary glands. Cancer 1984;54:1062-1069.
  4. Van der Waal JE, Becking AG, Snow GB, Van der Waal I; 2002, Distant metastasis of adenoid cystic carcinoma of the salivary glands and the value of diagnostic examinations during follow-up Head Neck 779-783.
  5. Brown DC, Gatter KC. Monoclonal antibody Ki-67: its use in histopathology Histopathology 1990;17:489-503.
  6. Hall PA, Levison DA. Review: Assessment of cell proliferation in histological material. J Clin Pathol 1990;43:184-192.
  7. Seifert G, Sobin LH. Histological classification of salivary gland tumours, in WHO International Histological Classification of Tumours. (ed 3) Lyon, 2005.
  8. Hirabayashi S. Immunohistochemical detection of DNA topoisomerase type II á and Ki-67 in adenoid cystic carcinoma and pleomorphic adenoma of the salivary gland. J Oral Pathol Med 1999;28:131-136.
  9. Okabe M, Inagaki H, Murase T, Inoue M, Nagai N, Eimoto T. Prognostic significance of p27 and Ki-67 expression in mucoepidermoid carcinoma of the intraoral minor salivary gland. Mod Pathol 2001;14(10):1008-1014.
  10. Gneep DR. Diagnostic surgical pathology of the head and neck. Philadelphia: WBSaunders. 2001;379.
  11. Nordgard S, Franzen G, Boysen M, Halvorsen TB. Ki-67 as a prognostic marker in adenoid cystic carcinoma assessed with the monoclonal antibody MIB-1 in paraffin sections. Laryngoscope 1997;107:5:31-36.
  12. Norberg-Spaak L, Dardick I, Ledin T Adenoid cystic carcinoma use of cell proliferation, Bcl-2 expression histological grade and clinical stage as predictors of clinical outcome. Head Neck 2000;22:489-497.
  13. Triantafillidou K, Dimitrakopoulos J, Iordanidis F, Koufogiannis. Management of Adenoid cystic carcinoma of minor salivary glands. J. Oral Maxillofac Surg 2006;64:1114-1120.
  14. Giannoni C, El-Naggar AK, Ordonez NG, Tu Zn, Austin J, Luna MA, Batakis JG. C-erb-2/neu oncogene and of palatal salivary gland neoplasms. Otolaryngol Head Neck Surg.1995;112:391-398.
  15. Carlinfante G, Lazzaretti M, Ferrari S, Bianchi b, Crafa B, P53 bcl-2 and Ki-67 expression in adenoid cystic carcinoma of palate. A clinicopathological study of 21 cases with long term follow up. Pathol. Res Pract 2005;200:791-799.
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.