Citation Information :
George AA, Muruppel AM, S S, N D, Lal S. A Comparative Evaluation of the Effectiveness of Three Different Modalities in Occluding Dentinal Tubules: An In Vitro Study. J Contemp Dent Pract 2019; 20 (4):454-459.
Aim: This in vitro study was designed to evaluate and compare the effectiveness of a dentifrice containing 8% arginine and calcium carbonate, diode laser (810 nm) and the combined use of diode laser (810 nm) and the same dentifrice in occluding dentinal tubules.
Materials and methods: Forty sectioned dentine discs were observed in this study. The Samples were randomly divided into 4 groups. In group A, a dentifrice containing arginine and calcium carbonate was applied. In group B, the specimens were subjected to diode laser (810 nm) irradiation. In group C, the same dentifrice was applied and the specimens were subjected to diode laser (810 nm) treatment. Group D was left untreated and served as the control. The specimens were observed under the scanning electron microscope. Then the photomicrographs of each specimen were taken which were uploaded into Photoshop 7®, and the diameters of the dentinal tubules of each specimen were measured and the data were analyzed statistically.
Results: The overall comparison between different treatment modalities using one-way analysis of variance (ANOVA) and multiple comparisons of two modalities assessed using post hoc Tukey method revealed that there is a highly significant difference (p value = 0.0001) in the dentinal tubule diameter and rate of occlusion in different treatment modalities.
Conclusion: The study concluded that all three treatment modalities caused significant occlusion of dentinal tubules. The combined use of desensitizing toothpaste and diode laser (810 nm) is the most effective, then comes the diode laser (810 nm) alone and finally the desensitizing toothpaste alone.
Clinical significance: This study suggests an effective treatment of dentinal hypersensitivity using diode laser and desensitizing toothpaste.
Dilsiz A, Aydýn T, Emrem G. Effects of the Combined Desensitizing Dentifrice and Diode Laser Therapy in the Treatment of Desensitization of Teeth with Gingival Recession. Photomed Laser Surg 2010;28: Supplement 2.
Gillam DG, Bulman JS, Eijkman MAJ, et al. Dentists’ perceptions of dentine hypersensitivity and knowledge of its treatment. J Oral Rehab 2002;29;219-225.
Rees JS, Addy M. A cross-sectional study of dentine hypersensitivity. J Clin Periodontol 2002;29:997-1003.
Rosenstiel SF, Rashid RG. Postcementation Hypersensitivity: Scientific Data Versus Dentists’ Perceptions. J Prosthodont 2003:12(2):73.
Cummins D. Recent advances in dentin hypersensitivity: Clinically proven treatments for instant and lasting sensitivity relief. Am J Dent 2010;23Sp Is A:3A-13A.
Mausner IK, Goldstein GR, Georgescu M. Effect of two dentinal desensitizing agents on retention complete cast coping using four cements. J Prosthet Dent 1996;129-134.
Yim NH, Rueggeberg FA, Caughman WF, et al. Effect of dentin desensitizers and cementing agents on retention of fullcrowns using standardized crown preparations. J Prosthet Dent 2000;83:459-465.
Cuenin MF, Scheidt MJ, O'Neal RB, et al. Horner and Thomas E. Van Dyke. An In vitro Study of Dentin Sensitivity and the Patency of Dentin Tubules. J Periodontol 1991;62:668-673.
Yoshiyama M, Masada J, Uchida A, et al. Scanning Electron Microscopic Characterization of Sensitive vs. Insensitive Human Radicular Dentin. J Dent Res 1989.68(11):1498-1502.
Wylie SG, Wilson PR. An Investigation into the Pressure Transmitted to the Pulp Chamber on Crown Cementation: A Laboratory Study. J Dent Res 1994.73(11):1684-1689.
Absi E G, Addy M, Adams D. Dentin hypersensitivity. A study of the patency of dentinal tubules in sensitive and non sensitive cervical dentin. J Clin Periodontol 1987;14:280-284.
Porto IC, Andrade AK, Montes MA. Diagnosis and Treatment of Dentinal Hypersensitivity. J Oral Sci 2009; 51(3):323-332.
Markowitz K, Pashley DH. Discovering new treatments for sensitive teeth: the long path from biology to therapy. J Oral Rehab 2008;35:300-315.
Sipahi C, Cehreli M, Ozen J, et al. Effects of precementation desensitizing laser treatment and conventional desensitizing agents on crown retention. Int J Prosthodont 2007;20(3):289-292.
Abdulhamaid A. Maghrabi. Effect of Dentin Sealers on Postoperative Sensitivity of Complete Cast Crowns Cemented with Glass Ionomer Cement. J Prosthodont 2011;20:385-390.
Sipahi C, Berk N, Ozen J, et al. Tubule-Occluding Effect of Desensitizing Laser Treatment on Prepared Dentin Surfaces: An Environmental SEM Study. Int J Prosthodont 2006;19:37-39.
Kishore A, Mehrotra KK, Saimbi CS. Effectiveness of Desensitizing Agents. Journal of Endodontics 2002;28(1):34-35.
Ling TY, Gillam DG, Barber PM, et al. An investigation of potential desensitizing agents in the dentine disc model: a scanning electron microscopy study. J Oral Rehab 1997;24;191-203.
Wolfart S, Wegner SM, Kern M. Comparison of using calcium hydroxide or a dentine primer for reducing dentinal pain following crown preparation: a randomized clinical trial with an observation time up to 30 months. Journal of Oral Rehabilitation 2004 31; 344-350.
Ajcharanukul O, Kraivaphan P, Wanachantararak S, et al. Effects of potassium ions on dentine sensitivity in man. Arch Oral Biol 2007;52:632-639.
Wara-aswapati N, Krongnawakul D, Jiraviboon D, et al. The effect of a new toothpaste containing potassium nitrate and triclosan on gingival health, plaque formation and dentine hypersensitivity. J Clin. Periodontol 2005;32:53-58.
Fu Y, Li X, Que K, Wang M, et al. Instant dentin hypersensitivity relief of a new desensitizing dentifrice containing 8.0% arginine, a high cleaning calcium carbonate system and 1450 ppm fluoride: A 3-day clinical study in Chengdu, China. Am J Dent 2010;23 Sp Is A:20A-27A.
Que K, Fu Y, Lin L, et al. Dentin hypersensitivity reduction of a new toothpaste containing 8.0% arginine and 1450 ppm fluoride: An 8-week clinical study on Chinese adults. Am J Dent 2010;23 Sp Is A:28A-35A.
Kimura Y, Wilder-Smith P, Yonaga K, et al. Treatment of dentine hypersensitivity by lasers: a review. J Clin Periodontol 2000;27: 715-721. 25 Hong L, Wu M, Zhang C. Using Nd-YAG laser to desensitize the hypersensitive dentine in preparing the abutment teeth for removable partial dentures prosthesis. Chinese J Stomatol 1997;32(1):28-30.
Zhang C, Matsumoto K, Kimura Y, et al. Effects of CO2 laser in the treatment of cervical dentinal hypersensitivity. J Endodont 1998;24(9):595-597.
Arrastia AM, Machida T, Smith PW, et al. Comparative study of the thermal effects of four semiconductor lasers on the enamel and pulp chamber of a human tooth. Lasers Surg Med 1994;15:382-389.
de Souza MR, Watanabe IS, Azevedo LH, et al. Morphological alterations of the surfaces of enamel and dentin of deciduous teeth irradiated with Nd:YAG, CO2 and diode lasers. Int J Morphol 2009; 27(2):441-446.
Badran Z, Boutigny H, Struillou X, et al. Tooth desensitization with an Er:YAG laser: in vitro microscopical observation and a case report. Lasers Med Sci 2011;26:139-142.
Lan WH, Liu HC, Lin CP. The Combined Occluding Effect of Sodium Fluoride Varnish and Nd:YAG Laser Irradiation on Human Dentinal Tubules. J Endodont 1999;25(6).
Schwarz F, Arweiler N, Georg T, et al. Desensitizing effects of an Er:YAG laser on hypersensitive dentine. A controlled, prospective clinical study. J Clin Periodontol 2002;29:211-215.
Lee BS, Chang CW, Chen WP, et al. In vitro study of dentin hypersensitivity treated by Nd- YAP Laser and Bioglass. Dent Mater 2005;21:511-519.
Lavender SA, Petrou I, Heu R, et al. Mode of action studies on a new desensitizing dentifrice containing 8.0% arginine, a high cleaning calcium carbonate system and 1450 ppm fluoride. Am J Dent 2010;23Sp Is A:14A-19A.
Peacock JM, Orchardson R. Effects of Potassium Ions on Action Potential Conduction in A- and C-Fibersof Rat Spinal Nerves. J Dent Res 1995;74(2):634-641.
Chow R, Armati P, Liisa Laakso E, et al. Inhibitory Effects of Laser Irradiation on Peripheral Mammalian Nerves and Relevance to Analgesic Effects: A Systematic Review. Photomed Laser Surg 2011;10(10):1-17.
Lee BS, Lin CP, Lin FH, et al. Ultra structural Changes of Human Dentin after Irradiation by Nd:YAG laser. Laser Surg Med 2002;30:246-252.
Sicilia A, Cuesta-Frechoso S, Suarez A, et al. Immediate efficacy ofdiode laser application in the treatment of dentine hypersensitivity in periodontal maintenance patients: a randomized clinical trial. J Clin Periodontol 2009;36:650-660.
Pesevska S, Nakova M, Ivanovski K, et al. Dentinal hypersensitivity following scaling and root planing: comparison of low-level laser and topical fluoride treatment. Lasers Med Sci 2009;Jun 1. 39 Marsilio AL, Rodrigues JR, Borges AB. Effect of the clinical application of the GaAlAs laser in the treatment of dentine hypersensitivity. J Clin Laser Med Surg 2003;21:291-296.
Johnson GH, Lepe X, Bales DJ. Crown retention with use of a 5% glutaraldehyde sealer on prepared dentin. J Prosthet Dent 1998;79:671-676.
Gholami GA, Fekrazad R, Esmaiel-Nejad A, et al. An Evaluation of the Occluding Effects of Er;Cr:YSGG, Nd:YAG, CO2 and Diode Lasers on Dentinal Tubules: A Scanning Electron Microscope In Vitro Study. Photomed Laser Surg 2011; 29(2): 115-121.
Kleinberg I, Kaufman HW, Confessore F. Methods of measuring tooth hypersensitivity. Dent Clin North Am. 1990;34(3):515-529.