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VOLUME 20 , ISSUE 6 ( June, 2019 ) > List of Articles


Cytogenetic Analysis of Micronuclei in Tobacco Chewers: A Study in North Indian Population

Neha Vaid, Deepak Bhargava, Puja Bansal, Rajeshwar Chawla, Chander US Pawar

Keywords : Biomarker, Micronuclei, Tobacco chewers

Citation Information : Vaid N, Bhargava D, Bansal P, Chawla R, Pawar CU. Cytogenetic Analysis of Micronuclei in Tobacco Chewers: A Study in North Indian Population. J Contemp Dent Pract 2019; 20 (6):693-693.

DOI: 10.5005/jp-journals-10024-2581

License: CC BY-NC 4.0

Published Online: 01-07-2019

Copyright Statement:  Copyright © 2019; The Author(s).


Aim: Oral cancer ranks in top three of all cancers in India and accounts for over 30% of all cancers reported in the country. The present study aims at an in vivo cytogenetic analysis to assess the applicability of the micronucleus test in diagnosing early detection of dysplastic changes. Materials and methods: Exfoliated scrape smears were collected from 75 patients suffering from premalignant lesions. The wet-fixed smears were stained by adopting Papanicolaou's staining protocol. The analysis of variance (ANOVA) test was used to analyze the data statistically. Results: The results of the above study give a fair assessment of the amount of nuclear alterations seen in individuals exposed to genotoxic agents, such as tobacco, and also to a certain extent, an insight into the expected biological behavior of the lesions present in such individuals. Detection of micronuclei and their assay is an upcoming research domain in the field of cancer detection and therapeutics. These miniature nuclear offshoots if properly identified can turn out to be important biomarkers with huge potential for screening and predicting patients with oral precancers and also can act as risk assessors in patient's ongoing treatment for invasive cancers. Conclusion: The frequency of micronucleated cells has been observed to be in increasing order with the increase in the age-groups and from control to precancerous cases significantly in both sexes.

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  1. Suhas S, Ganapathy KS, et al. Application of the micronucleus test to exfoliated epithelial cells from the oral cavity of beedi smokers, a high-risk group for oral cancer. Mutat Res 2004;561:15–21. DOI: 10.1016/j.mrgentox.2004.03.001.
  2. Holland N, Bolognesi C, et al. The micronucleus assay in human buccal cells as a tool for biomonitering DNA damage: the HUMN project perspective on current status and knowledge gaps. Mutat Res 2008;659(1–2):93–108. DOI: 10.1016/j.mrrev.2008.03.007.
  3. Motgi AA, Chavan MS, et al. Assessment of cytogenic damage in the form of micronuclei in oral epithelial cells in patients using smokeless and smoked form of tobacco and non-tobacco users and its relevance for oral cancer. J Cancer Res Ther 2014;10:165–170. DOI: 10.4103/0973-1482.131454.
  4. Sharma S, Rai S, et al. Quantitative and qualitative analysis of micronuclei in the buccal mucosal cells of individuals associated with tobacco. MAMC J Med Sci 2018;4(1):12–17.
  5. Somashekhar P, Kamath VV. Evaluation of micronuclei in betel quid chewers, potentially malignant oral disorders, and oral squamous cell carcinoma patients: a cytological assay. J Adv Clin Res Insights 2016;3(4):139–142.
  6. Sagari SG, Babannavar R, et al. Micronuclei frequencies and nuclear abnormalities in oral exfoliated cells of nuclear power plant workers. J Clin Diagn Res 2014 Dec;8(12):ZC15–ZC17. DOI: 10.7860/JCDR/2014/9059.5240.
  7. Tolbert PE, Shy CM, et al. Micronuclei and other nuclear anomalies in buccal smears: A Reid test in snuff users. Am J Epidemiol 1991;134(8):840–850.
  8. Uma AN, Dhananjay SK, et al. Comparative cytogenetic study of exfoliative oral mucosal cells in tobacco related potentially malignant disorders in a South Indian population. Int J Adv Res Technol 2014;3(8):6–12.
  9. Schmid W. The micronucleus test. Mutat Res 1975;31(1):9–15.
  10. Jadhav K, Gupta N, et al. Micronuclei: an essential biomarker in oral exfoliated cells for grading of oral squamous cell carcinoma. J Cytol 2011;28(1):7–12. DOI: 10.4103/0970-9371.76941.
  11. Caplash S, Meenakshi, et al. Micronucleus investigation in exfoliated buccal cells among tobacco chewers/smokers and controls. IJBPAS January 2013;2(1):72–79.
  12. Subramanian SV, Nandy S, et al. Patterns and distribution of tobacco consumption in India: cross sectional multilevel evidence from the 1998–1999 national family health survey. BMJ 2004;328:801–806. DOI: 10.1136/bmj.328.7443.801.
  13. Jyoti S, Khan S, et al. Micronucleus investigation in human buccal epithelial cells of gutkha users. Adv Biomed Res 2012;1(2):1–4.
  14. Sellappa S, Balakrishnan M, et al. Induction of micronuclei in buccal mucosa on chewing a mixture of betel leaf, areca nut and tobacco. J Oral Sci 2009;51(2):289–292.
  15. Palaskar S, Jindal C. Evaluation of micronuclei using Papanicolaou and May Grunwald Giemsa stain in individuals with different tobacco habits – A comparative study. J Clin Diagn Res 2010;4:3607–3613.
  16. Joshi MS, Verma Y, et al. Cytogenetic alterations in buccal mucosa cells of chewers of areca nut and tobacco. Arch Oral Biol 2011;56:63–67. DOI: 10.1016/j.archoralbio.2010.08.012.
  17. Dosi T, Gupta D, et al. Assessment of micronuclei frequency in individuals with a habit of tobacco by means of exfoliated oral buccal cells. J Int Soc Prev Community Dent 2016;6(2):S143–S147. DOI: 10.4103/2231-0762.189745.
  18. Bansal H, Sandhu VS, et al. Evaluation of micronuclei in tobacco users: a study in Punjabi population. Contemp Clin Dent 2012;3(2):184–187. DOI: 10.4103/0976-237X.96825.
  19. Ozkul Y, Donmez H, et al. Induction of micronuclei by smokeless tobacco on buccal mucosa cells of habitual users. Mutagenesis 1997;12(4):285–287.
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