Citation Information :
Rodrigues PD, Nassar RD, da Silva TS, Pedrinha VF, Alexandrino LD. Effects of Different NaOCl Concentrations Followed by 17% EDTA on Dentin Permeability. J Contemp Dent Pract 2019; 20 (7):838-841.
Aim: The aim of this study is to evaluate the permeability of root dentin after immersion in sodium hypochlorite (NaOCl) at different concentrations for 30 minutes and a final wash with 17% ethylenediaminetetraacetic acid (EDTA) for 3 minutes.
Materials and methods: Twenty 1 × 5 mm dentin fragments from the middle third of the root were prepared from 10 bovine teeth and divided into four groups; three of these groups were immersed in 1%, 2.5%, or 5.25% NaOCl for 30 minutes, while group IV was immersed in the saline solution. All dentin fragments were subjected to a final wash with 17% EDTA for 3 minutes. The fluid transport study model was used to measure the fluid conductance induced by hydrostatic pressure. The measurements were repeated twice to confirm the values, and in the case of a discrepancy, the procedure was performed again.
Results: The control group (saline solution) presented lower hydraulic conductance mean values with (0.25 ± 0.12). The 5.25% NaOCl followed by 17% EDTA produced the highest mean conductance value (1.18 ± 0.18) followed by 2.5% NaOCl and 17% EDTA with (0.81 ± 0.09) and (0.48 ± 0.02), respectively. The results of the hydraulic conductance evaluation of dentin were different for each NaOCl concentration. After data analyses, there were significant differences from analysis of variance (ANOVA) between all the groups (p < 0.0001).
Conclusion: The NaOCl solution concentration affects dentin permeability.
Vasconcelos LRSM, Midena RZ, et al. Effect of ultrasound streaming on the disinfection of flattened root canals prepared by Rotary and reciprocating systems. J Appl Oral Sci 2017 Oct;25(5):477–482. DOI: 10.1590/1678-7757-2016-0358.
Nogueira BML, Pereira TAC, et al. Effects of Different Irrigation Solutions and Protocols on Mineral Content and Ultrastructure of Root Canal Dentine. Iran Endod J 2018 Mar;13(2):209–215. DOI: 10.22037/iej.v13i2.19287.
Gu LS, Huang XQ, et al. Primum non nocere - the effects of sodium hypochlorite on dentin as used in endodontics. Acta Biomater 2017 Oct;61:144–156. DOI: 10.1016/j.actbio.2017.08.008.
Domínguez MCL, Pedrinha VF, et al. Effects of Different Irrigation Solutions on Root Fracture Resistance: An in Vitro Study. Iran Endod J 2018 Jul;13(3):367–372. DOI: 10.22037/iej.v13i3.19247.
Tartari T, Bachmann L, et al. Tissue dissolution and modifications in dentin composition by different sodium hypochlorite concentrations. J Appl Oral Sci 2016 Jun;24(3):291–298. DOI: 10.1590/1678-775720150524.
Tartari T, Guimarães BM, et al. Etidronate causes minimal changes in the ability of sodium hypochlorite to dissolve organic matter. Int Endod J 2015 Apr;48(4):399–404. DOI: 10.1111/iej.12329.
Uzunoglu E, Aktemir S, et al. Effect of ethylenediaminetetraacetic acid on root fracture with respect to concentration at different time exposures. J Endod 2012 Aug;38(8):1110–1113. DOI: 10.1016/j.joen.2012.04.026.
Zhang K, Tay FR, et al. The effect of initial irrigation with two different sodium hypochlorite concentrations on the erosion of instrumented radicular dentin. Dent Mater 2010 Jun;26(6):514–523. DOI: 10.1016/j.dental.2010.01.009.
Abuhaimed TS, Abou Neel EA. Sodium Hypochlorite Irrigation and Its Effect on Bond Strength to Dentin. Biomed Res Int 2017 Aug;2017:1–8. DOI: 10.1155/2017/1930360.
Breschi L, Maravic T, et al. Dentin bonding systems: From dentin collagen structure to bond preservation and clinical applications. Dent Mater 2018 Jan;34(1):78–96. DOI: 10.1016/j.dental.2017. 11.005.
Akisue E, Tomita VS, et al. Effect of the combination of sodium hypochlorite and chlorhexidine on dentinal permeability and scanning electron microscopy precipitate observation. J Endod 2010 May;36(5):847–850. DOI: 10.1016/j.joen.2009.11.019.
Mena-Serrano A, Costa TR, et al. Effect of sonic application mode on the resin-dentin bond strength and dentin permeability of self-etching systems. J Adhes Dent 2014 Jul;16(5):435–440.
Zhang K, Kim YK, et al. Effects of different exposure times and concentrations of sodiumhypochlorite/ethylenediaminetetraacetic acid on the structural integrity of mineralized dentin. J Endod Jan 2010;36(1):105–109. DOI: 10.1016/j.joen.2009.10.020.
Reeder OW, Walton RE, et al. Dentin Permeability: Determinants of Hydraulic Conductance. J Dent Res Feb 1978;57(2):187–193. DOI: 10.1177/00220345780570020601.
Siqueira JF, Rôças IN, et al. Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite. J Endod 2000 Jun;26(6):331–334. DOI: 10.1097/00004770-200006000-00006.
Cullen JKT, Wealleans JA, et al. The effect of 8.25% sodium hypochlorite on dental pulp dissolution and dentin flexural strength and modulus. J Endod 2015 Jun;41(6):920–904. DOI: 10.1016/j.joen.2015.01.028.
Dibaji F, Afkhami F, et al. Fracture Resistance of Roots after Application of Different Sealers. Iran Endod J 2017;12(1):50–54. DOI: 10.22037/iej.2017.10.
Sim T, Knowles J, et al. Effect of sodium hypochlorite on mechanical properties of dentine and tooth surface strain. Inter Endod J 2001 Mar;34(2):120–132. DOI: 10.1046/j.1365-2591.2001.00357.x.
Capar ID, Saygili G, et al. Effects of root canal preparation, various filling techniques and retreatment after filling on vertical root fracture and crack formation. Dent Traumatol 2015 Aug;31(4):302–307. DOI: 10.1111/edt.12154.
Kim DS, Kim J, et al. The influence of chlorhexidine on the remineralization of demineralized dentine. J Dent 2011 Dec;39(12):855–862. DOI: 10.1016/j.jdent.2011.09.010.
Palazzi F, Blasi A, et al. Penetration of sodium Hypochlorite Modified with Surfactants into Root Canal Dentin. Braz Dent J 2016 Mar-Apr;27(2):208–216. DOI: 10.1590/0103-6440201600650.
Mirseifinejad R, Tabrizizade M, et al. Efficacy of Different Root Canal Irrigants on Smear Layer Removal after Post Space Preparation: A Scanning Electron Microscopy Evaluation. Iran Endod J 2017;12(2):185–190. DOI: 10.22037/iej.2017.36.
Violich DR, Chandler NP. The smear layer in endodontics–a review. Int Endod J 2010;43(1):2–15. DOI: 10.1111/j.1365-2591.2009.01627.x.
Hulsmann M, Heckendorff M, et al. Comparative in vitro evaluation of three chelator pastes. Int Endod J 2002 Aug;35(8):668–679. DOI: 10.1046/j.1365-2591.2002.00543.x.
Qing Y, Akita Y, et al. Cleaning efficacy and dentin micro-hardness after root canal irrigation with a strong acid electrolytic water. J Endod 2006 Nov;32(11):1102–1106. DOI: 10.1016/j.joen.2006.07.003.
Taneja S, Kumari M, et al. Effect of QMix, peracetic acid and ethylenediaminetetraacetic acid on calcium loss and microhardness of root dentine. J Conserv Dent 2014 Mar-Apr;17(2):1155–1158. DOI: 10.4103/0972-0707.128058.
Tartari T, Bachmann L, et al. Analysis of the effects of several decalcifying agents alone and in combination with sodium hypochlorite on the chemical composition of dentine. Int Endod J 2018 Jan;51(1):42–54. DOI: 10.1111/iej.12764.
del Carpio-Perochena A, Bramante CM, et al. Effect of temperature, concentration and contact time of sodium hypochlorite on the treatment and revitalization of oral biofilms. J Dent Res Dent Clin Dent Prospects 2015 Dec;9(4):209–215. DOI: 10.15171/joddd.2015.038.