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VOLUME 20 , ISSUE 9 ( September, 2019 ) > List of Articles


An In Vitro Evaluation of Antibacterial Efficacy of Various Concentration of Eucalyptus globulus Leaf Extract on Periodontal Pathogens

Melwin Mathew, Sultan A Almalki, Ipsita Jayanti, Macha Durgaraju

Keywords : A. actinomycetemcomitans, Eucalyptus globulus, P. gingivalis, Zone of inhibition

Citation Information : Mathew M, Almalki SA, Jayanti I, Durgaraju M. An In Vitro Evaluation of Antibacterial Efficacy of Various Concentration of Eucalyptus globulus Leaf Extract on Periodontal Pathogens. J Contemp Dent Pract 2019; 20 (9):1041-1044.

DOI: 10.5005/jp-journals-10024-2639

License: CC BY-NC 4.0

Published Online: 01-12-2019

Copyright Statement:  Copyright © 2019; The Author(s).


Aim: The aim of the present study was to evaluate the antibacterial efficacy of various concentrations of Eucalyptus globulus leaf extract on periodontal pathogens. Materials and methods: Matured, healthy and disease-free leaves of Eucalyptus globulus were collected. The leaves were washed under tap water and finally dried in an oven at a temperature of 45°C for 48 hours. The dried plants were ground in an electric blender to make them into a powder. The powder was mixed with 100% ethanol and kept it inside a shaker overnight at 35°C. The mixture was centrifuged for 10 minutes at 2,500 rpm. Three different concentrations (10%, 50%, and 100% v/v) were used as antibacterial agents. Chlorhexidine (0.2%) was considered as positive control and dimethyl formamide was considered as negative control against P. gingivalis and A. actinomycetemcomitans. The disc diffusion method was used to determine the extract's antibacterial activity against the test organisms. A digital Vernier caliper was used to measure the diameter of antibacterial activity showing the zone of inhibition in millimeters. Results: Eucalyptus globulus with 100% concentration showed a maximum zone of inhibition against A. actinomycetemcomitans and P. gingivalis (5.38 ± 0.32 mm, 4.82 ± 0.11 mm) followed by 50% and 10% accordingly. The negative control of dimethyl formamide showed a zone of inhibition of 0.48 ± 0.96 mm and 0.63 ± 0.20 mm against A. actinomycetemcomitans and P. gingivalis. The positive control of 0.2% chlorhexidine showed a zone of inhibition of 8.46 ± 1.02 mm and 7.18 ± 0.54 mm against A. actinomycetemcomitans and P. gingivalis. The ANOVA test showed a highly significant antibacterial efficacy in 0.2% chlorhexidine and 100% concentration Eucalyptus globulus. Conclusion: A significant maximum zone of inhibition against A. actinomycetemcomitans and P. gingivalis was showed by 100% concentration of Eucalyptus globulus. Clinical significance: Other than the systemic diseases treatment, Eucalyptus globulus also serves as an effective promising alternative to antibiotics in the prevention of oral infections because of the natural phytochemicals existing in them.

  1. Menon L, Ramamurthy J. New vistas in plaque control. IOSR J Dent Med Sci 2014;13:64–68. DOI: 10.9790/0853-13356468.
  2. Pogue JM, Kaye KS, et al. Appropriate antimicrobial therapy in the era of multidrug-resistant human pathogens. Clin Microbiol Infect 2015;21:302–312. DOI: 10.1016/j.cmi.2014.12.025.
  3. Prabuseenivasan S, Jayakumar M, et al. In vitro antibacterial activity of some plant essential oils. BMC Complement Altern Med 2006;6:39. DOI: 10.1186/1472-6882-6-39.
  4. Pereira V, Dias C, et al. Antibacterial activity and synergistic effects between Eucalyptus globulus leaf residues (essential oils and extracts) and antibiotics against several isolates of respiratory tract infections (Pseudomonas aeruginosa). Ind Crops Prod 2014;52:1–7. DOI: 10.1016/j.indcrop.2013.09.032.
  5. Seyyed Nejad M, Motamedi H, et al. Antibacterial effect of Eucalyptus microtheca. Int J Enteric Pathog 2014;2(2):1–5.
  6. Nostro AM, Germano P, et al. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett Appl Microbiol 2000;30:379–384. DOI: 10.1046/j.1472-765x.2000.00731.x.
  7. Ravi K, Divyashree P. Psidium guajava: A review on its potential as an adjunct in treating periodontal disease. Pharmacogn Rev 2014;8:96–100. DOI: 10.4103/0973-7847.134233.
  8. Kshirsagar MM, Dodamani AS, et al. Antibacterial activity of garlic extract on cariogenic bacteria: An in vitro study. Ayu 2018;39:165–168. DOI: 10.4103/ayu.AYU_193_16.
  9. Nagata H, Inagaki Y, et al. Inhibitory effects of macrocarpals on the biological activity of Porphyromonas gingivalis and other periodontopathic bacteria. Oral Microbiol Immunol 2006;21:159–163. DOI: 10.1111/j.1399-302X.2006.00269.x.
  10. Hans VM, Grover HS, et al. Antimicrobial Efficacy of Various Essential Oils at Varying Concentrations against Periopathogen Porphyromonas gingivalis. J Clin Diagn Res 2016;10(9):ZC16–ZC19. DOI: 10.7860/JCDR/2016/18956.8435.
  11. Luqman S, Dwivedi GR, et al. Antimicrobial activity of Eucalyptus citriodora essential oil. Int J Essen Oil Ther 2008;2:69–75.
  12. Cimanga K, Kambu K, et al. Correlation between chemical composition and antibacterial activity of essential oils of some aromatic medicinal plants growing in the Democratic Republic of Congo. J Ethnopharmacol 2002;79:213–220. DOI: 10.1016/S0378-8741(01)00384-1.
  13. Silva J, Abebe W, et al. Analgesic and anti-inflammatory effects of essential oils of Eucalyptus. J Ethnopharmacol 2003;89:277–283. DOI: 10.1016/j.jep.2003.09.007.
  14. Tian Y, Liu X, et al. Extraction and determination of volatile constituents in leaves of Eucalyptus citriodora. Chinese J Chromatography 2005;23:651–654.
  15. Takarada K, Kimizuka R, et al. A comparison of the antibacterial efficacies of essential oils against oral pathogens. Oral Microbiol Immunol 2004;19:61–64. DOI: 10.1046/j.0902-0055.2003.00111.x.
  16. Motamayel FA, Hassanpour S, et al. Antibacterial effect of eucalyptus (globulus Labill) and garlic (Allium sativum) extracts on oral Cariogenic bacteria. J Microbiol Res Rev 2013;1(2):12–17.
  17. Nagata N, Inagaki Y, et al. Effect of eucalyptus extract chewing gum on periodontal health: a double-masked, randomized trial. J Periodontol 2008;79:1378–1385. DOI: 10.1902/jop.2008.070622.
  18. Cekici A, Kantarci A, et al. Inflammatory and immune pathways in the pathogenesis of periodontal disease. Periodontol 2000 2014;64(1):57–80. DOI: 10.1111/prd.12002.
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