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VOLUME 21 , ISSUE 1 ( January, 2020 ) > List of Articles

ORIGINAL RESEARCH

Evaluation of Efficacy of Injectable-guided Tissue Regeneration with and without Clindamycin on the Colonization of Porphyromonas gingivalis by Real-time Polymerase Chain Reaction

Voruganti Deepak, Johnson J Josephin, Palaparthy R Babu, Vikram R Guntakandla, Jagadish R Gooty, Suryakanth Malgikar, Abhinav Gupta

Keywords : Clindamycin, Injectable-guided tissue regeneration, Periodontitis, P. gingivalis, Real-time PCR

Citation Information : Deepak V, Josephin JJ, Babu PR, Guntakandla VR, Gooty JR, Malgikar S, Gupta A. Evaluation of Efficacy of Injectable-guided Tissue Regeneration with and without Clindamycin on the Colonization of Porphyromonas gingivalis by Real-time Polymerase Chain Reaction. J Contemp Dent Pract 2020; 21 (1):36-40.

DOI: 10.5005/jp-journals-10024-2742

License: CC BY-NC 4.0

Published Online: 01-01-2020

Copyright Statement:  Copyright © 2020; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim: Aim of the present study was to evaluate the loading of clindamycin with injectable-guided tissue regeneration (GTR) will prevent the colonization of Porphyromonas gingivalis and to compare and assess the quantitative changes in P. gingivalis colony forming units (CFUs) by real-time polymerase chain reaction (PCR) analysis. Materials and methods: Thirty microbiological samples were pooled from the deepest periodontal pockets from the thirty sites from the two groups: group I—injectable GTR placed in the defect filled with demineralized freeze-dried bone allograft (DFDBA) and group II—clindamycin loaded injectable GTR placed in the defect filled with DFDBA. The total number of P. gingivalis CFUs was estimated using real-time PCR at baseline and 4 weeks after therapy. Results: A significant reduction in P. gingivalis CFUs at the end of 4 weeks was seen in both groups. Comparative evaluations between both groups at 4 weeks were with a mean of 4.44 ± 2.28 and 4.75 ± 3.32, respectively. Though there was a significant reduction in group II, the difference was statistically insignificant. Conclusion: The results suggest that clindamycin is beneficial in reducing microbial infection and can potentiate regeneration through host modulation. Clinical significance: Injectable GTR has the ability to mold according to the defect size and shape and eliminates the need to manipulate the membrane as required for the conventional membrane.


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