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VOLUME 21 , ISSUE 10 ( October, 2020 ) > List of Articles

ORIGINAL RESEARCH

Staining the Ground Section of the Tooth Using an Innovative Plant Stain Found in the Pravara Region, India

Deepak M Vikhe, Sanjay G Thete, Chaitra S Mastud, Tejashree Mantri, Prasad N Mhaske, Santoshkumar P Mastud

Keywords : Ground section, Histopathology, Pravara stain

Citation Information : Vikhe DM, Thete SG, Mastud CS, Mantri T, Mhaske PN, Mastud SP. Staining the Ground Section of the Tooth Using an Innovative Plant Stain Found in the Pravara Region, India. J Contemp Dent Pract 2020; 21 (10):1113-1116.

DOI: 10.5005/jp-journals-10024-2904

License: CC BY-NC 4.0

Published Online: 08-01-2021

Copyright Statement:  Copyright © 2020; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aims: To assess the staining efficacy of the innovative “Pravara” stain on ground sections of human teeth under a stereomicroscope. In this study, the ground sections were stained with the innovative stain. The stain is obtained from a leafless tree called Terminalia chebula. Terminalia chebula, commonly known as Harad, is found in South Asia from India. Few species are found in the Pravara region of Maharashtra, India. Materials and methods: A total of 40 extracted teeth were collected and ground sections of each tooth were prepared. The samples were randomly divided into two groups: group I—control (without stain), group II—study group (the samples in this group were stained with the help of innovative “Pravara” stain). The sections were graded from I to III according to the differentiation and contrast of tissues. Results: The ground sections in group II stained with Pravara stain demonstrated better contrast for structures within sections under a microscope in comparison with group I (control group). Conclusion: The study demonstrates the efficacy of a new innovative Pravara stain, which is a simple, economical, quick, and effective staining method for the ground sections of the teeth. Clinical significance: This innovative Pravara stain is cost-effective, saves time, ecofriendly, and highlights the anatomical structures more effectively and does not fade easily with time.


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