The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 21 , ISSUE 11 ( November, 2020 ) > List of Articles

ORIGINAL RESEARCH

A Cross-sectional Study to Evaluate Nuclear Changes in Buccal Mucosa Following Panoramic Radiography

Kalaiselvi Santhosh, Sathick Manzoor, Anu Sushanth, Sakthidaran Seralathan, Vivekanandan Rajasekar, Anoop Jacob

Keywords : Buccal epithelium cells, Micronucleus test, Panoramic radiography, Radiation hazard

Citation Information : Santhosh K, Manzoor S, Sushanth A, Seralathan S, Rajasekar V, Jacob A. A Cross-sectional Study to Evaluate Nuclear Changes in Buccal Mucosa Following Panoramic Radiography. J Contemp Dent Pract 2020; 21 (11):1258-1261.

DOI: 10.5005/jp-journals-10024-2921

License: CC BY-NC 4.0

Published Online: 17-02-2021

Copyright Statement:  Copyright © 2020; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim and objective: To evaluate the possible genotoxic effect of X-rays on buccal mucosa while exposing to dental panoramic radiography using micronucleus test. Materials and methods: The study group comprised of 30 healthy subjects, 15 males and 15 females, aged between 24 years and 65 years. Samples were obtained from the exfoliated oral mucosa cells of buccal mucosa before and 12 days after exposing the patients to panoramic radiography. Results: The study reported that there was no significant increase in the number of micronuclei cells present before and after panoramic radiography. Positive correlation existed between age with pre- and postexposure micronuclei. Conclusion: Diagnostic dental panoramic radiograph does not induce micronuclei in the target buccal epithelium cells. A positive correlation between age and micronuclei frequency was established. Clinical significance: Panoramic radiographs does not induce cytotoxicity but increase frequency may be vulnerable to genotoxic effects in buccal mucosal cells. Hence, dental radiographs should be prescribed only when necessary.


PDF Share
  1. Genotoxic effects of X-rays on keratinized mucosa cells during panoramic dental radiography. Dentomaxillofac Radiol 2008;37(7):398–403. DOI: 10.1259/dmfr/56848097.
  2. Analysis of micronuclei in buccal epithelial cells in patients subjected to panoramic radiography. Indian J Dent Res 2012;23(5):574–578. DOI: 10.4103/0970-9290.107329.
  3. Genetic damage in exfoliated cells from oral mucosa of individuals exposed to X-rays during panoramic dental radiographies. Mutat Res 2004;562(1-2):111–117. DOI: 10.1016/j.mrgentox.2004.05.008.
  4. Mutagenicity and cytotoxicity assessment in patients undergoing orthodontic radiographs. Dentomaxillofac Radiol 2010;39(7):437–440. DOI: 10.1259/dmfr/24791952.
  5. Micronucleus test in buccal epithelium cells from patients subjected to panoramic radiography [published correction appears in Dentomaxillofac Radiol. 2008;37(1):62]. Dentomaxillofac Radiol 2007;36(3):168–171. Doi: 10.1259/dmfr/29193561.
  6. Micronuclei in exfoliated human cells as a tool for studies in cancer risk and cancer intervention. Cancer Lett 1984;22(3):241–253. DOI: 10.1016/0304-3835(84)90159-9.
  7. Cytokinesis-block micronucleus cytome assay. Nat Protoc 2007;2(5):1084–1104. DOI: 10.1038/nprot.2007.77.
  8. Evaluation of genotoxic effects due to panoramic radiograpy. J Indian Acad Oral Med Radiol 2015;27(1):25–28. DOI: 10.4103/0972-1363.167070.
  9. Nuclear changes in tongue epithelial cells following panoramic radiography. Mutat Res 2007;632(1-2):121–125. DOI: 10.1016/j.mrgentox.2007.05.003.
  10. The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: the humn project perspective on current status and knowledge gaps. Mutat Res 2008;659(1-2):93–108. DOI: 10.1016/j.mrrev.2008.03.007.
  11. Micronuclei and other nuclear anomalies in buccal smears: a field test in snuff users. Am J Epidemiol 1991;134(8):840–850. DOI: 10.1093/oxfordjournals.aje.a116159.
  12. What do human micronuclei contain? Mutagenesis 2003;18(3):221–233. DOI: 10.1093/mutage/18.3.221.
  13. The micronucleus assay in exfoliated human cells: a mini-review of papers from the CIS. Cytol Genet 2007;41(2):115–124. DOI: 10.3103/S0095452707020090.
  14. Micronucleus an upcoming marker of genotoxic damage. Clin Oral Investig 2007;11(2):121–126. DOI: 10.1007/s00784-006-0075-y.
  15. Genotoxic and cytotoxic effects of X-ray on buccal epithelial cells following panoramic radiography: a pediatric study. J Cytol 2015;32(2):102–106. DOI: 10.4103/0970-9371.160559.
  16. Cellular death but not genetic damage in oral mucosa cells after exposure to digital lateral radiography. Clin Oral Investig 2011;15(3):357–360. DOI: 10.1007/s00784-010-0402-1.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.