Comparison of the Antibacterial Activity of the Ethanol Extract vs Hydroalcoholic Extract of the Leaves of Mangifera indica L. (Mango) in Different Concentrations: An In Vitro Study
Citation Information :
Cardenas V, Chiong L, del Aguila E. Comparison of the Antibacterial Activity of the Ethanol Extract vs Hydroalcoholic Extract of the Leaves of Mangifera indica L. (Mango) in Different Concentrations: An In Vitro Study. J Contemp Dent Pract 2020; 21 (2):202-206.
Aim: To compare the in vitro antibacterial activity of different types of hydroalcoholic extracts of the leaves of the Mangifera indica L. (mango) plant on the strain of Staphylococcus aureus ATCC 6538™. Materials and methods: This study was experimental in vitro and determined the antibacterial activity of four dilutions: Mangifera indica L. ethanol extract (MEE) and Mangifera indica L. hydroalcoholic extract (MHE) at 50% and 100% on cultures of S. aureus ATCC 6538™ comparing with the positive control (chlorhexidine 0.12%) and negative (alcohol 96°) in Mueller Hinton agar cultures using the Kirby-Bauer diffusion method for each study group and incubating the cultures at 37°C for 24 hours. Results: It was found that the 50% and 100% MEE had a smaller size of the inhibitory halo of 21.3 ± 0.5 and 24.1 ± 0.8 mm, respectively. In addition, with respect to the 50% and 100% MHE, it was found that they had a higher antibacterial activity of 24.6 ± 0.5 and 33.5 ± 1.2 mm, respectively. Conclusion: Mango leaf extracts are potent antibacterial, proving 100% MHE to be more effective, thus confirming the presence of active constituents in medicinal plants. Clinical significance: This research has a great clinical applicability due to the opening of research lines that prove the usefulness of these extracts in the therapeutic control of certain oral diseases.
Medina D, Ulloa G, Camere R, et al. Antibacterial activity of Bixa orellana L. (achiote) against Streptococcus mutans and Streptococcus sanguinis. Asian Pac J Trop Biomed 2016;6(5):400–403. DOI: 10.1016/j.apjtb.2016.03.005.
Camere R, Ulloa G, Medina D, et al. Antibacterial activity of Myrciaria dubia (Camu camu) against Streptococcus mutans and Streptococcus sanguinis. Asian Pac J Trop Biomed 2016;6(9):740–744. DOI: 10.1016/j.apjtb.2016.07.008.
Mayta-Tovalino F, Gamboa E, Sanchez R, et al. Development and formulation of the experimental dentifrice based on Passiflora mollisima (Tumbo) with and without anion Fluor: antibacterial activity on seven antimicrobial strains. Int J Dent 2019;2019:9056590. DOI: 10.1155/2019/9056590.
Manzur AG, Sm Junior V, Morais-Costa F, et al. Extract of Mangifera indica L. leaves may reduce biofilms of Staphylococcus spp. in stainless steel and teatcup rubbers. Food Sci Technol Int 2020;26(1):11–20. DOI: 10.1177/1082013219858529.
Shafiei Z, Rahim ZHA, Philip K, et al. Potential effects of Psidium sp., Mangifera sp., Mentha sp. and its mixture (PEM) in reducing bacterial populations in biofilms, adherence and acid production of S. sanguinis and S. mutans. Arch Oral Biol 2020;109:104554. DOI: 10.1016/j.archoralbio.2019.104554.
Ameen F, Srinivasan P, Selvankumar T, et al. Phytosynthesis of silver nanoparticles using Mangifera indica flower extract as bioreductant and their broad-spectrum antibacterial activity. Bioorg Chem 2019;88:102970. DOI: 10.1016/j.bioorg.2019.102970.
Chirayath RB, A AV, Jayakumar R, et al. Development of Mangifera indica leaf extract incorporated carbopol hydrogel and its antibacterial efficacy against Staphylococcus aureus. Colloids Surf B Biointerfaces 2019;178:377–384. DOI: 10.1016/j.colsurfb.2019.03.034.
Valdivia-Rivera S, Varela-Santos EDC, Quiñones-Muñoz TA, et al. Production of hydrocarbon-degrading microorganisms using agricultural residues of Mangifera indica L. and Carica papaya as carbon source. 3 Biotech 2019;9(2):43. DOI: 10.1007/s13205-019-1574-2.
Bodiba DC, Prasad P, Srivastava A, et al. Antibacterial activity of Azadirachta indica, Pongamia pinnata, Psidium guajava, and Mangifera indica and their mechanism of action against Streptococcus mutans. Pharmacogn Mag 2018;14(53):76–80. DOI: 10.4103/pm.pm_102_17.
Subbiya A, Mahalakshmi K, Pushpangadan S, et al. Antibacterial efficacy of Mangifera indica L. kernel and Ocimum sanctum L. leaves against Enterococcus faecalis dentinal biofilm. J Conserv Dent 2013;16(5):454–457. DOI: 10.4103/0972-0707.117507.
Sundeep D, Vijaya Kumar T, Rao PSS, et al. Green synthesis and characterization of Ag nanoparticles from Mangifera indica leaves for dental restoration and antibacterial applications. Prog Biomater 2017;6(1–2):57–66. DOI: 10.1007/s40204-017-0067-9.
Shafiei Z, Haji Abdul Rahim Z, Philip K, et al. Antibacterial and anti-adherence effects of a plant extract mixture (PEM) and its individual constituent extracts (Psidium sp., Mangifera sp., and Mentha sp.) on single- and dual-species biofilms. Peer J 2016;4:e2519. DOI: 10.7717/peerj.2519.
Singh R, Singh SK, Maharia RS, et al. Identification of new phytoconstituents and antimicrobial activity in stem bark of Mangifera indica (L.). J Pharm Biomed Anal 2015;105:150–155. DOI: 10.1016/j.jpba.2014.12.010.
Hannan A, Asghar S, Naeem T, et al. Antibacterial effect of mango (Mangifera indica Linn.) leaf extract against antibiotic sensitive and multi-drug resistant Salmonella typhi. Pak J Pharm Sci 2013;26(4):715–719.
Rajan S, Thirunalasundari T, Jeeva S. Anti-enteric bacterial activity and phytochemical analysis of the seed kernel extract of Mangifera indica Linnaeus against Shigella dysenteriae (Shiga, corrig.) Castellani and Chalmers. Asian Pac J Trop Med 2011;4(4):294–300. DOI: 10.1016/S1995-7645(11)60089-8.
de Sousa Guedes JP, de Souza EL. Investigation of damage to Escherichia coli, Listeria monocytogenes and Salmonella enteritidis exposed to Mentha arvensis L. and M. piperita L. essential oils in pineapple and mango juice by flow cytometry. Food Microbiol 2018;76:564–571. DOI: 10.1016/j.fm.2017.09.020.
Poomanee W, Chaiyana W, Mueller M, et al. In vitro investigation of anti-acne properties of Mangifera indica L. kernel extract and its mechanism of action against Propionibacterium acnes. Anaerobe 2018;52:64–74. DOI: 10.1016/j.anaerobe.2018.05.004.
Vimalraj S, Ashokkumar T, Saravanan S. Biogenic gold nanoparticles synthesis mediated by Mangifera indica seed aqueous extracts exhibits antibacterial, anticancer and anti-angiogenic properties. Biomed Pharmacother 2018;105:440–448. DOI: 10.1016/j.biopha.2018.05.151.
Ediriweera MK, Tennekoon KH, Samarakoon SR. A review on ethnopharmacological applications, pharmacological activities, and bioactive compounds of Mangifera indica (Mango). Evid Based Complement Alternat Med 2017;2017:6949835. DOI: 10.1155/2017/6949835.
Sundararaman B, Muthuramu KL. A comparison of mango seed kernel powder, mango leaf powder and Manilkara zapota seed powder for decolorization of methylene blue dye and antimicrobial activity. J Environ Biol 2016;37(6):1315–1321.
Madduluri S, Babu Rao K, Sitaram B. In vitro evaluation of antibacterial activity of five indigenous plants extract against five bacterial pathogens of human. Int J Pharm Pharm Sci 2013;5(4):679–684.
Diso S, Ali M, Mukhtar S, et al. Antibacterial activity and phytochemical screening of Mangifera indica (Mango) stem and leaf extracts on clinical isolates of methicillin resistant Staphylococcus aureus. J Adv Med Pharm 2017;13(1):1–6. DOI: 10.9734/JAMPS/2017/31127.
Anand G, Ravinanthan M, Basaviah R, et al. In vitro antimicrobial and cytotoxic effects of Anacardium occidentale and Mangifera indica in oral care. J Pharm Bioallied Sci 2015;7(1):69–74. DOI: 10.4103/0975-7406.148780.
